中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3698-3702.doi: 10.3969/j.issn.1673-8225.2012.20.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

高脂饮食诱导肥胖模型大鼠骨骼肌组织蛋白酪氨酸磷酸酯酶1B和胰岛素受体底物2的表达★

赵  慧1，于苏国1，王令令2，赵艳敏1   

  1. 1滨州医学院附属医院内分泌科，山东省滨州市256603；2滨州市人民医院，山东省滨州市  256600
  • 收稿日期:2012-01-29 修回日期:2012-04-06 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 于苏国，硕士，副教授，滨州医学院附属医院内分泌科，山东省滨州市 256603 yusuguo@sina.com
  • 作者简介:赵慧★，女，1987年生，山东省阳信县人，汉族，2012年滨州医学院毕业，硕士，主要从事肥胖和胰岛素抵抗方面的研究。zhaohuisnow@163.com

Expression of protein-tyrosine phosphatase 1B and insulin receptor substrate 2 in the skeletal muscle of rats with insulin resistance induced by high-fat diet   

Zhao Hui1, Yu Su-guo1, Wang Ling-ling2, Zhao Yan-min1   

  1. 1Department of Endocrinology, Binzhou Medical University Hospital, Binzhou  256603, Shandong Province, China; 2Binzhou People’s Hospital, Binzhou  256600, Shandong Province, China
  • Received:2012-01-29 Revised:2012-04-06 Online:2012-05-13 Published:2012-05-13
  • Contact: Yu Su-guo, Master, Associate professor, Department of Endocrinology, Binzhou Medical University Hospital, Binzhou 256603, Shandong Province, China yusuguo@sina.com
  • About author:Zhao Hui★, Master, Department of Endocrinology, Binzhou Medical University Hospital, Binzhou 256603, Shandong Province, China zhaohuisnow@163.com

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摘要：

背景：外周组织的胰岛素抵抗是2型糖尿病的主要病因。
目的：观察高脂饮食诱导的肥胖大鼠骨骼肌中蛋白酪氨酸磷酸酯酶1B和胰岛素受体底物2的表达。
方法：将20只SD大鼠随机等分为对照组和高脂组，分别给予常规饲料和高脂饲料喂养12周。
结果和结论：与对照组相比，高脂组大鼠胰岛素敏感指数显著降低(P < 0.01)，大鼠葡萄糖耐量受损，胰岛素释放试验提示葡萄糖刺激的胰岛素第一时相分泌受损，骨骼肌组织中蛋白酪氨酸磷酸酯酶1B蛋白表达水平明显增加(P < 0.01)，骨骼肌中胰岛素诱导的胰岛素受体底物2磷酸化程度降低(P < 0.01)。提示高脂饮食诱导的肥胖大鼠骨骼肌中蛋白酪氨酸磷酸酯酶1B蛋白表达量升高，使胰岛素诱导的胰岛素受体底物2磷酸化程度降低，可能是肥胖导致胰岛素抵抗的机制之一。
 
关键词：肥胖；蛋白酪氨酸磷酸酶1B；胰岛素受体底物2；骨骼肌；胰岛素抵抗
doi:10.3969/j.issn.1673-8225.2012.20.020

关键词: 肥胖, 蛋白酪氨酸磷酸酶1B, 胰岛素受体底物2, 骨骼肌, 胰岛素抵抗

Abstract:

BACKGROUND: Insulin resistance in the peripheral tissue is a major cause of type 2 diabetes mellitus.
OBJECTIVE: To observe the expression of protein tyrosine phosphatase 1B (PTP-1B) and insulin receptor substrate 2 (IRS-2) in the skeletal muscle of rats with insulin resistance induced by high-fat diet.
METHODS: Twenty SD rats were randomly divided into normal control group with 10 rats and high-fat diet group with 10 rats. Rats in the two groups were fed with normal diet and high-fat diet for 12 weeks, respectively.
RESULTS AND CONCLUSION: The insulin sensitive index was significantly decreased in the high-fat diet rats compared with the normal rats (P < 0.01). In obese rats, glucose tolerance and the acute first-phase insulin secretory response to glucose were impaired. The protein level of PTP-1B in the skeletal muscle of obese rats was significantly increased compared with the control group (P < 0.01). Insulin-stimulated IRS-2 phosphorylation in the skeletal muscle was reduced in the obese rats (P < 0.01). These indicate that the increased PTP-1B level and the reduced insulin-stimulated IRS-2 phosphorylations in the skeletal muscle seem to play an important role in the insulin resistance induced by high-fat diet.

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