中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (20): 3675-3679.doi: 10.3969/j.issn.1673-8225.2012.20.015

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预*★

张宪发1，吴正球2，梁自乾1，赵学凯1，丁华荣1   

  1. 1广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市   530021；2宾阳县人民医院中医外科，广西壮族自治区南宁市   530405
  • 收稿日期:2011-11-02 修回日期:2012-01-10 出版日期:2012-05-13 发布日期:2012-05-13
  • 通讯作者: 吴正球，副主任医师，宾阳县人民医院中医外科，广西壮族自治区南宁市 530405 wzhq6767@163.com
  • 作者简介:张宪发★，男，1983年生，山东省汶上县人，汉族，广西医科大学在读硕士，主要从事创伤修复和烧伤感染方面的研究。 zxiana2003@163.com
  • 基金资助:

    广西研究生教育创新计划基金(2010105981002M187)。

Effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats  

Zhang Xian-fa1, Wu Zheng-qiu2, Liang Zi-qian1, Zhao Xue-kai1, Ding Hua-rong1   

  1. 1Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Department of Traditional Chinese Medicine Surgery, Binyang People’s Hospital, Nanning  530405, Guangxi Zhuang Autonomous Region, China
  • Received:2011-11-02 Revised:2012-01-10 Online:2012-05-13 Published:2012-05-13
  • Contact: Wu Zheng-qiu, Department of Traditional Chinese Medicine Surgery, Binyang People's Hospital, Nanning 530405, Guangxi Zhuang Autonomous Region, China wzhq6767@163.com
  • About author:Zhang Xian-fa★, Studying for master’s degree, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zxiana2003@163.com
  • Supported by:

    Graduate Education Innovation Fund of Guangxi Zhuang Autonomous Region, No. 2010105981002 M187* 
       

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摘要：

背景：研究证明龙血竭有促进创伤愈合、抗血小板聚集、抗炎镇痛、抗菌抗氧化等生物活性。
目的：验证龙血竭在糖尿病大鼠烫伤皮肤组织愈合过程中对P物质和Bcl-2表达的影响。
方法：Wistar大鼠112只，随机分为4组。龙血竭组、磺胺嘧啶银(SD-Ag)组、糖尿病对照组和正常对照组。其中前3组为糖尿病深Ⅱ度烫伤模型，正常对照组为正常深Ⅱ度烫伤模型。
结果与结论：烫伤后第7天开始创面愈合率龙血竭组、正常对照组高于SD-Ag组和糖尿病对照组(P < 0.05)，第21天龙血竭组创面愈合率明显高于SD-Ag组和糖尿病对照组(P < 0.05)，正常对照组最高。烫伤后第15天正常对照组P物质阳性表达水平最高，峰值显著高于其他3组，以后逐步减弱。龙血竭组、SD-Ag组和糖尿病对照组于烫伤后第21天阳性表达最强，且龙血竭组表达明显高于SD-Ag组和糖尿病对照组(P < 0.05)。烫伤后第3天开始正常对照组Bcl-2阳性表达水平明显高于其他3组；于第15天各组阳性表达水平皆达到峰值，龙血竭组最高，明显高于SD-Ag组和糖尿病对照组(P < 0.05)，与正常对照组比较差异无显著性意义(P > 0.05)。说明龙血竭能通过参与调控P物质和Bcl-2的表达，有效地促进糖尿病烫伤创面的愈合。

关键词: 龙血竭, 糖尿病, 烫伤, P物质, Bcl-2

Abstract:

BACKGROUND: Previous studies have demonstrated that dragon’s blood can promote the wound healing as well as have characteristics of anti-platelet aggregation, anti-inflammatory analgesic, anti-bacterial and antioxidant and other biological activity.
OBJECTIVE: To identify the effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats.
METHODS: A total of 112 Wistar rats were randomly divided into four groups: dragon’s blood group, silver sulfadiazine (SD-Ag) group, diabetic control group and normal control group. Diabetic deep Ⅱ degree burn models were established in dragon’s blood group, SD-Ag group and diabetic control group, and the normal deep Ⅱ degree burn models were established in normal control group.
RESULTS AND CONCLUSION: On the 7th day post scald, the wound healing rates of dragon’s blood group and normal control group were higher than those of SD-Ag group and diabetic control group (P < 0.05). After 21 days, the wound healing rate of dragon’s blood group was significantly higher than that of SD-Ag group and diabetic control group (P < 0.05) and highest in normal control group. After 15 days, the positive expression level of substance P reached a peak in normal control group and was higher than other three groups, and then decreased gradually. The expression was most positive in dragon’s blood group, SD-Ag group and diabetic control group at the 21th day post scald, and which was significantly higher in dragon’s blood group (P < 0.05). On the 3rd day after scald, the expression level of Bcl-2 in the normal control group was significantly higher than that in other groups. From the 15th day, the expression of Bcl-2 was gradually increased in each group and reached to peak, and highest in dragon’s blood group (P < 0.05), but there was no significant difference between dragon’s blood group and normal control group (P > 0.05). The results demonstrate that dragon's blood can promote the healing of diabetic burn wounds significantly by regulating the expression of substance P and Bcl-2.

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