中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2750-2756.doi: 10.3969/j.issn.1673-8225.2012.15.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

去卵巢骨质疏松模型大鼠的番茄红素干预

杨希重1，季翠杰2，王德春1，陈晓亮1   

  1. 1青岛大学医学院附属医院脊柱外科，山东省青岛市  266003；2 即墨市人民医院神经内科，山东省即墨市  266200
  • 收稿日期:2011-10-11 修回日期:2012-01-13 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 陈晓亮，硕士，教授，硕士生导师，青岛大学医学院附属医院脊柱外科，山东省青岛市 266003
  • 作者简介:杨希重，男，1972年生，2008年于青岛大学医学院毕业，山东省蓬莱市人，汉族，主治医师，主要从事脊柱外科工作。现在在山东省即墨市人民医院工作。

Antiosteoporotic activity of lycopene in mature ovariectomized rats

Yang Xi-zhong1, Ji Cui-jie2, Wang De-chun1, Chen Xiao-liang1   

  1. 1Department of Spine Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao  266003, Shandong Province, China; 2Department of Neurology, People’s Hospital of Jimo City, Jimo  266200, Shandong Province, China
  • Received:2011-10-11 Revised:2012-01-13 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Chen Xiao-liang, Master, Professor, Master’s supervisor, Department of Spine Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
  • About author:Yang Xi-zhong, Attending physician, Department of Spine Surgery, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China yxzh001@hotmail.com

PDF

533

被引次数

11

摘要：

背景：有研究表明番茄红素作为一种抗氧化物质能够减低患慢性疾病的风险。
目的：建立去卵巢骨质疏松大鼠模型，通过给去卵巢大鼠灌服不同剂量的番茄红素，观察其对实验性骨质疏松的保护作用。
方法：切除6月龄SPF级雌性Wistar 未生育大鼠双侧卵巢1周，建立去卵巢骨质疏松大鼠模型，分别给予苯甲酸雌二醇，番茄红素10 mg，20 mg进行干预。
结果与结论：各组给药12周后，番茄红素
 
高低剂量组与卵巢切除组比较子宫质量，血清中的钙、磷含量，骨小梁面积百分比，骨小梁数目增加，血清中超氧化物歧化酶活性，骨密度和骨生物学性能均明显增高       (P < 0.05)；血清中碱性磷酸酶，尿中脱氧吡啶啉，丙二醛，骨小梁分离度和破骨细胞数均减少(P < 0.05)。结果证实，番茄红素能明显缓解去卵巢引起的子宫萎缩，清除体内的自由基，改善去卵巢大鼠的氧化应激状态，减少骨吸收增加骨形成。
 

关键词: 番茄红素, 去卵巢, 氧化应激, 骨质疏松, 大鼠, 骨组织构建

Abstract:

BACKGROUND: A number of studies have suggested that lycopene is an antioxidant which is used to decrease the risk of age-related chronic diseases.
OBJECTIVE: To establish a rat model of postmenopausal osteoporosis, and to investigate the effects of lycopene in preventing bone loss in ovatiectomized rat models of osteoporosis.
METHODS: The 6-month-old female Wistar rats, SPF grade, which had never given birth, were divided into sham and ovatiectomized groups. The ovatiectomized rats were further sub-divided into four groups which were administered with corn oil orally as model group, lycopene (10 mg/kg, 20 mg/kg, daily) or treated by intraperitoneal injection with estradiol benzoate        (25 mg/kg, twice).
RESULTS AND CONCLUSION: After 12-week administration, as compared with the model group, the uterus weight of high-dose and low-dose lycopene groups significantly increased (P < 0.05); the Ca, P, superoxide dismutase levels, percent trabecular area, and trabecular number were obviously higher, and serum alkaline phosphatase, urine deoxypyridinoline, malondialdehyde, trabecular separation and number of osteoclasts were obviously lower in the lycopene groups than the model group (P < 0.05). The bone mass index, bone bio-mechanics and bone histomorphometry were better in the lycopene groups than the model group    (P < 0.05). These findings indicate that lycopene has a definite antiosteoporotic effect on ovatiectomized rats.

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