中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (10): 1554-1559.doi: 10.3969/j.issn.2095-4344.2014.10.012

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐带间充质干细胞体外诱导分化为成纤维细胞

杨  一,罗  新,蒋学风,帅翰林,宋  泓,张敬丽,蓝建发   

  1. 暨南大学附属第一医院妇产科,广东省广州市  510630
  • 出版日期:2014-03-05 发布日期:2014-03-05
  • 通讯作者: 罗新,博士,主任医师,教授,暨南大学附属第一医院妇产科,广东省广州市 510630
  • 作者简介:杨一,女,1987年生,湖北省咸宁市人,汉族,暨南大学在读硕士,主要从事妇科泌尿与盆底重建外科方面的研究。
  • 基金资助:

    国家自然科学基金面上项目(81070459);广东省科技厅科研基金项目(2007B06041054);广东省自然科学基金博士科研启动项目(粤科基办字[2008]4号8451063201000290);广东省医学科研基金立项课题(粤卫[2008] 84号A2008364);广东省医学科研基金项目(粤卫[2007]97号A2007338)

Human umbilical cord mesenchymal stem cells are induced in vitro to differentiate into fibroblasts

Yang Yi, Luo Xin, Jiang Xue-feng, Shuai Han-lin, Song Hong, Zhang Jing-li, Lan Jian-fa   

  1. Department of Gynecology and Obstetrics, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
  • Online:2014-03-05 Published:2014-03-05
  • Contact: Luo Xin, M.D., Chief physician, Professor, Department of Gynecology and Obstetrics, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
  • About author:Yang Yi, Studying for master’s degree, Department of Gynecology and Obstetrics, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81070459; the Science and Technology Fund of Guangdong Science and Technology Bureau, No. 2007B06041054; the Natural Science Foundation of Guangdong Province, No. 8451063201000290; the Medical Scientific Research Foundation of Guangdong Province, No. A2008364, A2007338

摘要:

背景:脐带间充质干细胞具有多向分化能力,但其向成纤维细胞分化研究较少。
目的:验证人脐带间充质干细胞向成纤维细胞的分化能力。
方法:采用贴壁法分离脐带间充质干细胞,流式细胞仪分析其表面抗原。取第3代脐带间充质干细胞进行成脂成骨诱导分化,以碱性成纤维细胞生长因子诱导脐带间充质干细胞向成纤维细胞分化。
结果与结论:贴壁法能稳定从脐带中分离出干细胞,脐带间充质干细胞极低表达 CD31、CD45 、CD40、HLA-DR,强表达 CD29、CD90、CD44、CD105。脐带间充质干细胞成脂诱导后油红O染色显示胞浆中充满红色的油滴;成骨诱导后茜素红染色可在细胞密集区见红色的钙结节。碱性成纤维细胞生因子诱导后细胞表达Ⅰ型胶原明显高于对照组。提示贴壁法分离脐带间充质干细胞可靠、纯度高,碱性成纤维细胞生长因子可诱导脐带间充质干细胞向成纤维细胞分化。


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

关键词: 干细胞, 脐带脐血干细胞, 间充质干细胞, 脐带间充质干细胞, 碱性成纤维细胞生长因子, 成纤维细胞, 国家自然科学基金

Abstract:

BACKGROUND: The umbilical cord mesenchymal stem cells possess multipotent differentiation capacity, but less research focus on its differentiation into fibroblasts.
OBJECTIVE: To investigate the capacity of human umbilical cord mesenchymal stem cells differentiating into fibroblasts.
METHODS: Using adherent method, human umbilical cord mesenchymal stem cells were isolated, and flow cytometric analysis of the surface antigen was performed. Passage 3 cells were selected for osteogenic and adipogenic differentiation, and cells differentiated into fibroblasts under the induction of basic fibroblast growth factor.
RESULTS AND CONCLUSION: Adherent stem cells were stably isolated from the umbilical cord. Human umbilical cord mesenchymal stem cells lowly expressed CD31, CD45, CD40, HLA-DR, but strongly expressed CD29, CD90, CD44, CD105. Oil red O staining showed red droplets were full of the cytoplasm after adipogenic induction; alizarin red staining showed red calcium nodules after osteogenic induction. After induced by basic fibroblast growth factor, the type I collagen expression was significantly higher than that in the control group. These findings indicate that the adherent human umbilical cord mesenchymal stem cells are reliably isolated with high purity; basic fibroblast growth factor can induce differentiation of umbilical cord mesenchymal stem cells into fibroblasts.


中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程


全文链接:

Key words: stem cells, mesenchymal stem cells, umbilical cord, fibroblasts

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