中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 1056-1061.doi: 10.3969/j.issn.1673-8225.2012.06.024

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

2型糖尿病合并急性脑梗死外周血CD34+水平变化及其意义★

周晓映1，于红艳2，王惠青1，原淑鸿1，高江彦1，许  蕾3   

  1. 1河北省胸科医院内科，河北省石家庄市  050041；  2河北工程附属医院内科，河北省邯郸市  056002；  3河北医科大学附属二院神经内科，河北省石家庄市  050000
  • 收稿日期:2011-08-16 修回日期:2011-10-17 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 许蕾，河北医科大学附属二院神经内科，河北省石家庄市050000 XLDOC@126.com
  • 作者简介:周晓映★，女，1971年生，河北省饶阳县人，汉族，1994年河北医学院毕业，硕士，副主任医师，主要从事2型糖尿病合并急性脑梗死外周血CD34+水平变化研究。mu-0101@163.com

Changes of peripheral blood CD34+ level in type 2 diabetes combined with acute cerebral infraction and its clinical significance

Zhou Xiao-ying1, Yu Hong-yan2, Wang Hui-qing1, Yuan Shu-hong1, Gao Jiang-yan1, Xu Lei3   

  1. 1Department of Internal Medicine, Chest Hospital of Hebei Province, Shijiazhuang  050041, Hebei Province, China; 2Department of Internal Medicine, the Affiliated Hospital of Hebei Engineering University, Handan  056002, Hebei Province, China; 3Department of Neurology, Second Affiliated Hospital of Hebei Medical University, Shijiazhuang  050000, Hebei Province, China
  • Received:2011-08-16 Revised:2011-10-17 Online:2012-02-05 Published:2012-02-05
  • Contact: Xu Lei, Department of Neurology, Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China XLDOC@126.com
  • About author:Zhou Xiao-ying★, Master, Associate chief physician, Department of Internal Medicine, Chest Hospital of Hebei Province, Shijiazhuang 050041, Hebei Province, China mu-0101@163.com

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摘要：

背景：CD34+细胞是一种新的血管内皮标志物，通过检测CD34+细胞水平可以反映血管内皮功能的变化。
目的：分析外周血CD34+细胞数量变化与脑梗死发病的关系。
方法：纳入观察对象110例，分为4组，2型糖尿病组、急性脑梗死组、2型糖尿病合并急性脑梗死组各30例，健康对照组20例。应用流式细胞仪ProCOUNT方法测定外周血CD34+/外周血单核细胞值，分析外周血CD34+/单核细胞值与脑血管病危险因素的相关性。
结果与结论：4组外周血CD34+/单核细胞值经过方差分析，差异具有显著性意义(P < 0.05)，健康对照组高于2型糖尿病组、急性脑梗死组、2型糖尿病合并脑梗死组(P < 0.01)，2型糖尿病组高于急性脑梗死组、2型糖尿病合并脑梗死组(P < 0.01)。2型糖尿病合并急性脑梗死患者外周血CD34+/单核细胞值与空腹血糖和收缩压呈负相关，与高密度脂蛋白胆固醇/低密度脂蛋白胆固醇的比值呈正相关，与舒张压、吸烟、胆固醇、三酰甘油无相关性。提示CD34+细胞可能使脑血管病危险因素受到影响从而参与了脑梗死的发生，监测CD34+细胞水平变化也许能用来预测2型糖尿病患者急性脑梗死的发生。

关键词: 急性脑梗死, CD34+细胞, 2型糖尿病, 外周血, 危险因素

Abstract:

BACKGROUND: CD34+ cell is a new marker of vascular endothelium, the detection of CD34+ cells level may reflect the changes of endothelial function.
OBJECTIVE: To analyze the relationship between the number of CD34+ cells and the cerebral infarction.
METHODS: A total of 110 subjects were included to review, the subjects were divided into four groups: type 2 diabetes group, acute cerebral infraction group, combination group (n=30) and control group (n=20). Flow cytometry Pro COUNT method was used to measure the number of peripheral blood CD34+ mononuclear cells and analyze the relationship with the risk factors for cerebrovascular disease.
RESULTS AND CONCLUSION: There was significant difference in the number of peripheral blood CD34+ mononuclear cells of four groups (P < 0.05) analyzed by variance. The number of peripheral blood CD34+ mononuclear cells in control group was higher than that in other three groups (P < 0.01), the type 2 diabetes group was higher than acute cerebral infraction group and combination group (P < 0.01). The number of peripheral blood CD34+ mononuclear cells in patients with type 2 diabetes combined with acute cerebral infraction was negative correlated with fasting plasma glucose and systolic blood pressure, and positively correlated with the ratio of high-density lipoprotein cholesterol/low-density lipoprotein cholesterol. It had no relationship with diastolic blood pressure, smoking, cholesterol and triglyceride. CD34+ cells can influence the cerebral infarction development by its risk factors, so the monitoring of CD34+ can be used to prevent the occurrence of acute cerebral infarction in type 2 diabetes patients.

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