中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 9885-9888.doi: 10.3969/j.issn.1673-8225.2011.53.002

• 肝移植 liver transplantation • 上一篇    下一篇

受体来源肝卵圆细胞在移植肝内种植后的增殖与分化

李  铸1，陈  娟1，李学华1，刘志恒1，刘桂杰1，张学利1，高延超1，孙会东1，纪丕友1，李  立2，张金良1   

  1. 1聊城市人民医院(泰山医学院附属聊城医院)肝胆外科，山东省聊城市 252000
    2昆明市第一人民医院(昆明医学院附属甘美医院)肝胆胰一病区，云南省昆明市  650011
  • 收稿日期:2011-08-13 修回日期:2011-09-05 出版日期:2011-12-31 发布日期:2011-12-31
  • 通讯作者: 李立，教授，博士生导师，昆明市第一人民医院（昆明医学院附属甘美医院）肝胆胰一病区，云南省昆明市 650011 ynkmlili@yahoo.com.cn
  • 作者简介:李铸☆，男，1976年生，陕西省镇巴县人，2010年昆明医学院毕业，博士，主治医师，主要从事肝胆胰外科及肝移植的基础与临床研究。 lllllzhu@126.com

Proliferation and differentiation of recipient-sourced hepatic oval cells in liver graft after cell seeding

Li Zhu1, Chen Juan1, Li Xue-hua1, Liu Zhi-heng1, Liu Gui-jie1, Zhang Xue-li1, Gao Yan-chao1, Sun Hui-dong1,Ji Pi-you1, Li Li2, Zhang Jin-liang1   

  1. 1Department of Hepatopancreatobiliary Surgery, the People’s Hospital of Liaocheng, Liaocheng Hospital Affiliated to Taishan Medical University, Liaocheng  252000, Shandong Province, China
    2First Ward, Department of Hepatopancreatobiliary Surgery, the First People’s Hospital of Kunming, Ganmei Hospital Affiliated to Kunming Medical University, Kunming  650011, Yunnan Province, China
  • Received:2011-08-13 Revised:2011-09-05 Online:2011-12-31 Published:2011-12-31
  • Contact: Li Li, Professor, Doctoral supervisor, First Ward, Department of Hepatopancreatobiliary Surgery, the First People’s Hospital of Kunming, Ganmei Hospital Affiliated to Kunming Medical University, Kunming 650011, Yunnan Province, China ynkmlili@yahoo.com.cn
  • About author:Li Zhu☆, Doctor, Attending physician, Department of Hepatopancreatobiliary Surgery, the People’s Hospital of Liaocheng, Liaocheng Hospital Affiliated to Taishan Medical University, Liaocheng 252000, Shandong Province, China lllllzhu@126.com

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摘要：

背景：肝卵圆细胞移植后对受损肝脏具有修复作用。
目的：观察受体来源肝卵圆细胞对受体大鼠术后生存期及肝功能的影响。
方法：采用二袖套法建立大鼠原位肝移植模型，供体为雌性DA大鼠，受体为雌性Lewis大鼠。造模后随机分为对照组(不进行细胞移植)、实验组(移植过程中供肝经门静脉和肝动脉注射受体来源肝卵圆细胞1×109 L-1)。
结果与结论：实验组术后撤他克莫司后的中位生存期和累积生存率均优于对照组(P < 0.05)，移植肝肝细胞受损情况弱于对照组(P < 0.05)，移植肝合成和排泄功能明显好于对照组(P < 0.05)。表明在移植肝脏内种植受体来源肝卵圆细胞可有效改善移植肝功能，并且明显延长受体生存时间，提高累积生存率，该结果可能与受体来源肝卵圆细胞在移植肝内增殖分化，修复受损肝脏和减轻急性排斥反应有关。

关键词: 肝卵圆细胞, 肝移植, 大鼠, 生存时间, 肝功能   

Abstract:

BACKGROUND: Hepatic oval cells (HOCs) exhibit repair effects on liver injury after cell seeding.
OBJECTIVE: To observe the effects of recipient-sourced HOCs on survival time and liver function of recipient rats after liver transplantation and HOC seeding.
METHODS: Rats orthotopic liver transplantation models were established by Kamada two-cuff technique. Female DA rats served as donors, and female Lewis rats were used as recipients. Recipients were randomly divided into a control group (no cell seeding) and an experimental group (recipient-sourced HOCs seeding through portal vein and hepatic artery during liver transplantation, 1×109/L).
RESULTS AND CONCLUSION: The median survival time and accumulated survival rate in the experimental group were better than those in the control group (P < 0.05). Degree of hepatocyte injury in the experimental group was mild than that in the control group (P < 0.05), and liver synthetic function and liver excretion function in the experimental group were much better than those in the control group (P < 0.05). These findings suggest that seeding of recipient-sourced HOCs into liver graft can effectively improve liver function, obviously prolong median survival time and increase accumulated survival rate in recipients after liver transplantation. These results might be related to the proliferation and differentiation of recipient-sourced HOCs in liver graft, the repair of liver injury and alleviated acute rejection.

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