中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (41): 7787-7790.doi: 10.3969/j.issn.2095-4344.2012.41.037

• 干细胞临床实践 clinical practice of stem cells • 上一篇    

脐血单个核细胞移植治疗痉挛型小儿脑性瘫痪的安全性

徐 蓉1，刘 波2，段 答2，樊梦瑶2，刘 斌1，滕晓华2，赵振宇2，卢 明2   

  1. 1湖南师范大学医学院，湖南省长沙市 410006
    2湖南师范大学第二附属医院，解放军第163医院神经外科，湖南省长沙市 410003
  • 收稿日期:2012-01-09 修回日期:2012-01-29 出版日期:2012-10-07 发布日期:2012-10-07
  • 通讯作者: 卢明，教授，主任医师，湖南师范大学第二附属医院，解放军第163医院神经外科，湖南省长沙市 410003 lming163@yahoo.com.cn
  • 作者简介:徐蓉★，女，1986年生，湖南省常德市人，汉族，2012年湖南师范大学医学院毕业，硕士，医师，主要从事神经修复相关究。 xurong0706@163.com

Transplantation of umbilical cord mononuclear cells for treatment of infantile spastic cerebral palsy

Xu Rong1, Liu Bo2, Duan Da2, Fan Meng-yao2, Liu Bin1, Teng Xiao-hua2, Zhao Zhen-yu2, Lu Ming2   

  1. 1Medical College of Hunan Normal University, Changsha 410006, Hunan Province, China
    2Department of Neurosurgery, Second Affiliated Hospital of Hunan Normal University,the163 Hospital of Chinese PLA, Changsha 410003, Hunan Province, China
  • Received:2012-01-09 Revised:2012-01-29 Online:2012-10-07 Published:2012-10-07
  • Contact: Lu Ming, Professor, Chief physician, Department of Neurosurgery, Second Affiliated Hospital of Hunan Normal University,the163 Hospital of Chinese PLA, Changsha 410003, Hunan Province, China lming163@yahoo.com.cn
  • About author:Xu Rong★, Master, Physician, Medical College of Hunan Normal University, Changsha 410006, Hunan Province, China xurong0706@163.com

PDF

424

被引次数

5

摘要：

背景：大量研究证实脐血间充质干细胞体外培养可被诱导分化为表达神经元和神经胶质细胞表面标志物的细胞，对神经系统疾病起到替代修复的作用。
目的：观察人脐血单个核细胞移植治疗痉挛型小儿脑性瘫痪的疗效和安全性。
方法：收集正常足月剖腹产胎儿的脐血，经肝素抗凝，用淋巴细胞分离液分离脐血单个核细胞，采用差速贴壁法进行纯化，并经腰椎穿刺注入30例痉挛型脑性瘫痪患者体内。
结果与结论：移植后患者肢体的肌肉痉挛程度和关节活动度明显改善，与移植前相比差异有显著性意义(P < 0.05)，移植后和随访过程中无不良反应和并发症。

关键词: 脐血间充质干细胞, 脑性瘫痪, 肌张力, 关节活动度, 疗效, 细胞移植, 干细胞

Abstract:

BACKGROUND: A large number of studies have demonstrated that umbilical cord mesenchymal stem cells cultured in vitro can express the surface markers of neurons and glial cells under induction and exhibit replacing and repairing effects for nervous system disease.
OBJECTIVE: To study the therapeutic effect and safety of transplantation of human umbilical cord blood mononuclear cells in treatment of infantile spastic cerebral palsy.
METHODS: Umbilical cord blood was harvested from full term deliveries scheduled for cesarean section and anti-coagulated with heparin. Umbilical cord blood mononuclear cells were isolated by lymphocyte separation medium, purified by Nash differential adhesion, and then injected into subarachnoid cavity of 30 infantile patients with spastic cerebral palsy.
RESULTS AND CONCLUSION: Compared with prior to treatment, the muscle spastic and joint range of motion significantly improved after treatment (P < 0.05). There were no adverse events and complications after transplantation and during follow-up period.

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