中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9531-9534.doi: 10.3969/j.issn.1673-8225.2011.51.008

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜的制备及降解性能

吴岳恒1，张灵敏2，曾祥君3，麦丽萍1，武长礼3，汤顺清2，余细勇1   

  1. 1广东省医学科学院 广东省人民医院医学研究中心，广东省广州市  510080
    2暨南大学生物医学工程所，广东省广州市  510632
    3广东省医学科学院 广东省心血管病研究所心外科，广东省广州市  510080
  • 收稿日期:2011-10-12 修回日期:2011-10-31 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 余细勇，博士，教授，博士生导师，广东省医学科学院 广东省人民医院医学研究中心，广东省广州市 510080 yuxycn@ hotmail.com
  • 作者简介:吴岳恒★，男，1980年生，湖南省株洲市人，汉族，2005年暨南大学毕业，硕士，助理研究员，主要从事生物材料和辅助循环的研究。 edgar_wu@ yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30901468)，课题名称：偏磷酸钙微粒改善多孔聚四羟丁酸膜细胞亲和性的实验研究。

Preparation and degradation property of composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate

Wu Yue-heng1, Zhang Ling-min2, Zeng Xiang-jun3, Mai Li-ping1, Wu Chang-li3, Tang Shun-qing2, Yu Xi-yong1   

  1. 1Center of Medical Research, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou  510080, Guangdong Province, China
    2Institute of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
    3Department of Cardiac Surgery, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou  510080, Guangdong Province, China
  • Received:2011-10-12 Revised:2011-10-31 Online:2011-12-17 Published:2011-12-17
  • Contact: Yu Xi-yong, Doctor, Professor, Doctoral supervisor, Center of Medical Research, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China yuxycn@hotmail.com
  • About author:Wu Yue-heng★, Master, Assistant researcher, Center of Medical Research, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China edgar_wu@ yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30901468*

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摘要：

背景：种子细胞能否成功黏附于支架材料表面，与支架材料的降解性能密切相关。
目的：制备多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜，并观察其体外降解性能。
方法：将聚3-羟基丁酸-4-羟基丁酸和偏磷酸钙按50∶50混合均匀，完全溶解于氯仿后，搅拌均匀，倾倒在培养皿上，干燥后即成多孔膜。将其置于循环装置中，流速为0.30 m/s，管路内灌满37 ℃生理盐水50 mL。
结果与结论：流场作用多孔复合膜2，4，8，16，24 h后，其失重率分别为4.5%，5.6%，6.9%，9.6%和10.6%；扫描电镜显示，降解24 h后，表面偏磷酸钙微粒已降解完全或脱落，而内凹偏磷酸钙微粒仍嵌入在聚3-羟基丁酸-4-羟基丁酸复合膜内，复合膜表面直径2 μm的微孔基本不见，直径30~50 μm的大孔基本未变，但孔隙边缘变得圆滑；水静力称重法计算出降解实验前后复合膜吸水率、显气孔率和密度分别为降解前的40.6%，46.3%，1.14 g/cm3和降解后的51.6%，54.1%，1.05 g/cm3。说明实验制备的多孔聚3-羟基丁酸-4-羟基丁酸/偏磷酸钙复合膜降解早期以偏磷酸钙降解为主，降解过程中孔内微丝易降解或断裂。

关键词: 聚3-羟基丁酸-4-羟基丁酸, 降解, 偏磷酸钙, 复合膜, 材料, 吸水率, 显气孔率, 密度

Abstract:

BACKGROUND: Degradation properties of scaffold are closely related to the adhering of seed cells to its surface.
OBJECTIVE: To prepare the composite membrane of porous poly (3-hydroxybutyrate-co-4-hydroxybutyrate)/calcium metaphosphate (P (3HB-co-4HB)/CMP) and observe its degradation properties in vitro.
METHODS: The raw material ratio of P (3HB-co-4HB)/CMP was 50/50, and the P (3HB-co-4HB)/CMP was mixed uniformly and dissolved in chloroform and dumped in a Petri dish. After drying, the porous composite membranes were obtained. The porous composite membranes were put in the circulation device, the flow rate of which was 0.30 m/s, the channel was filled with 50 mL 37 ℃ physiological saline.
RESULTS AND CONCLUSION: Weight loss rates of the composite membrane at 2, 4, 8, 16, 24 hours under flow field were 4.5%, 5.6%, 6.9%, 9.6% and 10.6% respectively; scanning electron microscopy showed that the partial calcium phosphate particles on the surfaces were complete depredated or lost, the concave particles were kept, 2 μm porous on the surfaces could not be seen, 30-50 μm porous did not change but the edge was smooth; the water absorption, porosity and density of the composite membrane were 40.6%, 46.3%, and 1.14 g/cm3 respectively before depredation, and 51.6%, 54.1%, 1.05 g/cm3 after that. During the preparation of P (3HB-co-4HB)/CMP, the depredation of the composite membrane in the early stage is the partial calcium phosphate depredation, and the microfilament is prone to be depredated and facture during the depredation.

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