中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (41): 7621-7624.doi: 10.3969/j.issn.2095-4344.2012.41.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

小鼠骨髓来源Flk-1+间充质干细胞分离制备与生物学特点

朱希山，姜 妮，宋雨光，任 军   

  1. 首都医科大学附属北京世纪坛医院(北京大学第九临床医学院)肿瘤内科，北京市 100038
  • 收稿日期:2012-03-05 修回日期:2012-06-23 出版日期:2012-10-07 发布日期:2012-10-07
  • 作者简介:朱希山☆，男，山东省潍坊市人，汉族，2009年北京协和医学院(清华大学医学部)毕业，博士，主要从事肿瘤干细胞的基础研究和临床应用。 zhuxishan@tsinghua.org.cn

Isolation and preparation of Flk-1+ mesenchymal stem cells from mice and its biological characteristics

Zhu Xi-shan, Jiang Ni, Song Yu-guang, Ren Jun   

  1. Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China
  • Received:2012-03-05 Revised:2012-06-23 Online:2012-10-07 Published:2012-10-07
  • About author:Zhu Xi-shan☆, Doctor, Department of Medical Oncology, Beijing Shijitan Hospital of Capital Medical University (Ninth Clinical Medical College of Peking University), Beijing 100038, China zhuxishan@tsinghua.org.cn

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摘要：

背景：骨髓间充质干细胞具有广泛的应用前途，其三系分化的能力和免疫诱导特性使其具有再生医学的应用前景。
目的：从小鼠骨髓中研究分离制备Flk-1+间充质干细胞的新方法，检测了这类细胞的生物特性。
方法：以小鼠骨髓为研究对象，分离单个核细胞，体外培养并 扩增原始间充质干细胞，检测它们的细胞周期、表型和多系分化的能力。
结果与结论：小鼠来源的骨髓来源的原始间充质干细胞呈成纤维样生长，大部分细胞处于G0/G1期，并且高表达Flk1，CD13，CD29，CD44，小鼠骨髓来源Flk-1+间充质干细胞在光镜下均呈成纤维样或多角型贴壁生长，在体外相应的诱导液环境中均可以向三系分化。提示小鼠骨髓来源Flk-1+间充质干细胞具有多向分化潜能。

关键词: 骨髓, 间充质干细胞, Flk-1+间充质干细胞, 小鼠, 细胞周期, 表型, 分化, 干细胞

Abstract:

BACKGROUND: The bone marrow mesenchymal stem cells can be widely used, the three-lineage differentiation capacity and immune induction characteristics make it widely used in regenerative medicine.
OBJECTIVE: To investigate the new method to isolate the Flk-1+ mesenchymal stem cells from mouse bone marrow, and to detect the biological characteristics of Flk-1+ mesenchymal stem cells.
METHODS: The mononuclear cells were isolated from the bone marrow and the original mesenchymal stem cells were in vitro cultured and amplified. The cell cycle, phenotype and multilineage differentiation capacity were detected.
RESULTS AND CONCLUSION: The original mesenchymal stem cells derived from mice bone marrow were fibrolbast like, and most of them stayed in G0/G1 phase. Cell phenotype test showed they could highly express Flk1, CD13, CD29, CD44; the bone marrow-derived Flk-1+ mesenchymal stem cells under light microscope showed a fibroblast-like or polygonal adherent growth, and under certain circumstances, they exhibited osteogenic, adipogenic and chondrogenic differentiation. Bone marrow-derived Flk-1+ mesenchymal stem cells have multilineage differentiation potential.

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