中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6745-6748.doi: 10.3969/j.issn.1673-8225.2011.36.022

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

心肌营养素1对谷氨酸诱导神经干细胞凋亡的作用

沈栋林1，束晓梅2，李振宏2，陈雪梅2，杜淑珍2   

  1. 1徐州医学院附属医院儿科，江苏省徐州市 221002
    2 遵义医学院第一附属医院儿科，贵州省遵义市563003
  • 收稿日期:2011-03-23 修回日期:2011-06-23 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 束晓梅，博士，主任医师，遵义医学院附属第一附属医院儿科，贵州省遵义市 563003 shuxiaomei1993@sina.com
  • 作者简介:沈栋林★，男，1973年生，江苏省徐州市人，汉族，2010年遵义医学院毕业，硕士，主治医师，主要从事神经干细胞与临床应用的研究。 sdlin99@sina.com
  • 基金资助:

    贵州省优秀人才省长资金资助 (2005-86)；遵义医学院博士启动资金资助(2004-010)。

Effects of cardiotrophin-1 on glutamate induced apoptosis of neural stem cells

Shen Dong-lin1, Shu Xiao-mei2, Li Zhen-hong2, Chen Xue-mei2, Du Shu-zhen2   

  1. 1Department of Pediatrics, Affiliated Hospital of Xuzhou Medical College, Xuzhou  221002, Jiangsu Province, China
    2Department of Pediatrics, the First Affiliated Hospital, Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2011-03-23 Revised:2011-06-23 Online:2011-09-03 Published:2011-09-03
  • Contact: Shu Xiao-mei, Doctor, Chief physician, Department of Pediatrics, the First Affiliated Hospital, Zunyi Medical College, Zunyi 563003, Guizhou Province, China shuxiaomei1993@sina.com
  • About author:Shen Dong-lin★, Master, Attending physician, Department of Pediatrics, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China sdlin99@sina.com
  • Supported by:

    Guizhou Governor Fund for Talents, No. 2005-86*; the Doctoral Start-up Fund of Zunyi Medical College, No. 2004-010*

PDF

302

被引次数

6

摘要：

背景：心肌营养素1属于白细胞介素6细胞因子家族，近年发现其可能对中枢神经系统有重要保护作用。
目的：探索重组腺病毒心肌营养素1对谷氨酸诱导神经干细胞凋亡的保护作用，为脑损伤提供新的治疗措施。
方法：取新生Wistar大鼠海马神经干细胞，第2代培养第5天用于实验，神经干细胞分为3组：谷氨酸组、重组腺病毒心肌营养素1组及对照组，利用四唑蓝比色了解细胞存活情况及原位缺口末端标记技术检测细胞凋亡。
结果与结论：谷氨酸组神经干细胞呈现明显形态改变，重组腺病毒心肌营养素1组未见类似改变，在谷氨酸暴露后12，24，48及72 h MTT吸光度值均低于心肌营养素1组，而细胞凋亡率均高于心肌营养素1组。与其他两组比较，对照组相同时间点A值增高，细胞凋亡率降低。提示，心肌营养素1对谷氨酸损伤的神经干细胞具有保护作用，可以抑制神经干细胞凋亡，促进其存活和增殖。

关键词: 心肌营养素1, 神经干细胞, 凋亡, 谷氨酸, 增殖

Abstract:

BACKGROUND: Cardiotrophin-1 (CT-1) appertains to interleukin-6 cytokine family, which is found in recent years to have an important protective role in the central nervous system.
OBJECTIVE: To observe the protective effects of CT-1 on the apoptosis of neural stem cells (NSCs) induced by glutamate.
METHODS: Hippocampal neural stem cells isolated from newborn Wistar rats were passaged and divided into 3 groups: glutamate, CT-1 (transfected with Adv CT-1) and control groups. NSCs survivals were estimated by MTT colorimetric method, and the apoptosis of NSCs was detected by means of TdT-Mediated dUTP nick end labeling (TUNEL).
RESULTS AND CONCLUSION: Glutamate treatment alone induced cell death that was associated with obvious morphological changes, while these changes were inhibited by transfection of CT-1 into NSCs. At 12, 24, 48 and 72 hours after treatment with glutamate, the absorbance values of MTT in the lutamate group were significantly lower than those in the CT-1 group, the apoptosis rates of NSCs in the glutamate group were significantly higher than those in the CT-1 group. At the same time, the absorbance values of MTT in the control group were significantly higher than those in the glutamate group and CT-1 group, the apoptosis rates of NSCs were significant lower than those in the glutamate group and CT-1 group. These indicated that CT-1 has a protective effect on NSCs injured by glutamate, can inhibit NSCs apoptosis and improve NSCs survival.

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