关键词: 肾阴虚证, 绝经后骨质疏松症, 基因表达, 功能分类, 信号通路, 基因组学, 组织构建

Abstract:

BACKGROUND: Gene differential expression exists in various types of postmenopausal osteoporosis, but its genomics mechanism has not been studied and reported.
OBFECTIVE: Through analyzing the gene expression spectrum’s difference, to investigate characteristics of genes expression profiles of postmenopausal osteoporosis with Kidney Yin deficiency.
METHODS: By TCM syndrome, 13 patients with postmenopausal osteoporosis were randomly divided into three groups: kidney Yin deficiency (n=4), kidney Yang deficiency (n=3), non-kidney deficiency (n=3), another 3 healthy postmenopausal women also were selected as control group. Human gene expression microarrays were applied to explore gene expression spectrum's difference of the groups. Kidney Yin deficiency group was compared with other three groups respectively. The genes with common significant difference were studied by Go and Pathway analysis.
RESULTS AND CONCLUSION: Compared with control group, kidney Yang deficiency and non-kidney deficiency groups, there were 197, 354 and 54 genes expressed differentially in the kidney Yin deficiency group. Nine genes were expressed differentially among kidney Yin deficiency group with other three groups: PROK2, ASB1, MUC12, GSTM5, CLCF1, GPR27, C3orf35 and unknown genes chr3:113822408-11382234, chr18:34851804-34851745. Compared with control group, GSTM5 and MUC12 showed up-regulated expression, GPR27, C3orf35, ASB1, CLCF1 and PROK2 showed down-regulated expression. The pathway analysis showed GSTM5 was involved in glutathione metabolism, and CLCF1 was involved in cytokine-cytokine receptor interaction and Jak-STAT signaling. The pathogenesis of postmenopausal osteoporosis with kidney Yin deficiency is associated with the genes expression of GPR27, ASB1, PROK2, CLCF1 and GSTM5, and it has association with glutathione metabolism pathway, cytokine-cytokine receptor interaction pathway and Jak-STAT signaling pathway.