中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (15): 2703-2706.doi: 10.3969/j.issn.1673-8225.2011.15.011

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

激素性股骨头坏死模型兔血管内皮细胞生长因子表达与普伐他汀的干预

胡  敏1，范建楠2，赵宏斌3，钱传云3，魏  蔚3，刘燕琨4，张  扬3   

  1. 1昆明学院医学院，云南省昆明市 650214
    2贵阳医学院附属医院骨科，贵州省贵阳市550004
    3昆明医学院第一附属医院骨科，云南省昆明市  650032
    4昆明市科学技术局，云南省昆明市   650500
  • 收稿日期:2010-12-04 修回日期:2011-03-06 出版日期:2011-04-09 发布日期:2013-11-06
  • 通讯作者: 赵宏斌，博士，副教授，昆明医学院第一附属医院骨科，云南省昆明市 650032 576794680@qq.com
  • 作者简介:胡敏，男，1971年生，云南省石屏县人，汉族，1995年于昆明医学院毕业，副教授，主要从事骨质疏松和骨坏死的临床和基础研究。 humin999@ yahoo.cn 并列第一作者：范建楠，男，1967年生，贵州省独山县人，彝族，1991年华西医科大学毕业，副教授，主要从事骨科临床工作。
  • 基金资助:

    国家自然科学基金(81060361，30860389)课题名称：恒古骨伤愈合剂对实验性骨质疏松性骨折的治疗作用及其细胞分子机制研究及从STI及破骨相关基因表达的关系探讨彝药恒古骨伤愈合剂对骨质疏松性骨折愈合质量的影响；云南省应用基础研究面上项目(2010ZC169)，课题名称：骨质疏松性骨折关键基因表达及恒古骨伤愈合剂干预研究；云南省教育厅科学研究基金项目(2010C212)，课题名称：恒古骨伤愈合剂对培养的小鼠成骨细胞增殖和分化的影响；昆明市科技计划重点项目 (10S090202)，课题名称：恒古骨伤愈合剂治疗骨质疏松性骨折的临床前基础应用研究。

Interventional effect of pravastatin on vascular endothelial growth factor expression in rabbits with steroid-induced avascular necrosis of the femoral head

Hu Min1, Fan Jian-nan2, Zhao Hong-bin3, Qian Chuan-yun3, Wei Wei3, Liu Yan-kun4, Zhang Yang3   

  1. 1Clinical Specialty, Medical College of Kunming University, Kunming  650214, Yunnan Province, China
    2Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China
    3Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China
    4Kunming Science and Technology Agency, Kunming  650500, Yunnan Province, China
  • Received:2010-12-04 Revised:2011-03-06 Online:2011-04-09 Published:2013-11-06
  • Contact: Zhao Hong-bin, Doctor, Associate professor, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China 576794680@qq.com
  • About author:Hu Min, Associate professor, Clinical Specialty, Medical College of Kunming University, Kunming 650214, Yunnan Province, China humin999@ yahoo.cn Fan Jian-nan, Associate professor, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China Hu Min and Fan Jian-nan contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No. 81060361*, 30860389*; Natural Science Foundation of Yunnan Province, No. 2010ZC169*; Research Foundation of Department of Education of Yunnan Province, No.2010C212*; Research Foundation of Science and Technology of Kunming, No.10S090202*

PDF

416

被引次数

5

摘要：

背景：有研究表明他汀类药物可使体外培养的人脐静脉内皮细胞的血管内皮细胞生长因子呈高表达，血管内皮细胞生长因子可促进内皮细胞增殖，明显提高成骨细胞活性并加速骨形成。
目的：观察普伐他汀对激素性股骨头坏死兔模型血管内皮生长因子的蛋白表达的影响。
方法：将新西兰白兔随机分为对照组，模型组和普伐他汀组。模型组和普伐他汀组制备兔激素性股骨头缺血坏死模型。普伐他汀组建模成功后以普伐他汀(1.2 mg/kg)灌胃，1次/d，模型组和对照组以等体积的蒸馏水灌胃。于造模后8，12，16周截取各组股骨头行免疫组织化学检测血管内皮生长因子蛋白的表达情况。
结果与结论：对照组不同时间点股骨头血管内皮生长因子蛋白表达均为阳性。造模后8周，模型组血管内皮生长因子蛋白表达呈阴性表达，普伐他汀组呈弱阳性表达。造模后12周，模型组和普伐他汀组血管内皮生长因子蛋白表达呈阳性表达，16周呈弱阳性表达。结果证实，普伐他汀可有效促进早期激素性股骨头坏死兔模型坏死股骨头内源性血管内皮生长因子的蛋白表达。

关键词: 普伐他汀, 血管内皮生长因子, 激素性股骨头坏死, 免疫组织化学, 组织构建

Abstract:

BACKGROUND: Previous research has demonstrated that pravastatin can effectively promote endogenous vascular endothelial growth factor (VEGF) mRNA in endothelial cells from human umbilical vein cultured in vitro, but the research of protein VEGF expression in the femoral heads of rabbits with steroid-induced avascular necrosis of the femoral head (SANFH) after treatment with pravastatin is rare now.
OBJECTIVE: To evaluate the endogenous protein VEGF expression in the femoral heads of SANFH rabbit models after treatment with pravastatin.
METHODS: Totally 80 New Zealand white rabbits were randomly divided into a normal control group (n=18) and experimental group (n=62). Rabbits in the experimental group were prepared for SANFH models. At 5 weeks after model preparation, 36 rabbits in the model group were assigned into the model and pravastatin groups, with 18 animals in each group. Rabbits in the pravastatin group received intragastric administration with 1.2 mg/kg pravastatin once per day, and the same volume of distilled water was given to those in the model and control groups. Six rabbits in each group were killed at the 8th, 12th and 16th week respectively to evaluation of protein VEGF expression in the femoral heads by immunohistochemistry.
RESULTS AND CONCLUSION: In the normal control group, endogenous expression of VEGF mRNA was positive at each time point. In the model group, 8 weeks after modeling, there was negative VEGF mRNA expression; 12 weeks after modeling positive expression; 16 weeks after modeling weakly positive expression. In the pravastatin group, 8 weeks after modeling, VEGF mRNA showed weak positive expression; 12 weeks after modeling positive expression; 16 weeks after modeling positive expression, but lower than 12 weeks after modeling slightly weakened. Pravastatin can effectively promote endogenous VEGF mRNA in early SANFH rabbit models.

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