中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4476-4480.doi: 10.3969/j.issn.1673-8225.2012.24.022

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

基质细胞衍生因子1对转染CXCR4基因支气管上皮细胞的增殖和 定向趋化作用

刘 军，任开明，石文君   

  1. 中国医科大学附属盛京医院，辽宁省沈阳市 110004
  • 收稿日期:2011-12-02 修回日期:2012-02-25 出版日期:2012-06-10 发布日期:2013-11-05
  • 作者简介:刘军★，男，1974年生，辽宁省大连市人，汉族，1998年中国医科大学毕业，硕士，副教授，主要从事气管替代物的研究。 junliu110@163.com
  • 基金资助:

    辽宁省教育厅高等学校科研基金资助项目(L2010636)

Effects of stromal cell derived factor-1 on the proliferation and directional migration of bronchial epithelial cells transfected by CXCR4 gene

Liu Jun, Ren Kai-ming, Shi Wen-jun   

  1. Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China
  • Received:2011-12-02 Revised:2012-02-25 Online:2012-06-10 Published:2013-11-05
  • About author:Liu Jun★, Master, Associate professor, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China junliu110@163.com

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摘要：

背景：早期的诱导替代物表面的气管黏膜上皮覆盖并恢复其功能，是气管替代物研究中的关键问题。基质细胞衍生因子1/CXCR4通路在加快组织修复中发挥重要作用。
目的：观察基质细胞衍生因子1对转染CXCR4基因支气管上皮细胞的增殖和定向趋化作用。
方法：构建CXCR4慢病毒表达载体并转染人支气管上皮细胞。实验分为3组，空白组：未感染任何病毒的细胞组；对照组：感染未转染CXCR4的慢病毒细胞组；实验组：转染CXCR4慢病毒表达载体的细胞组。将消化好的病毒浸染的人支气管上皮细胞和普通人支气管上皮细胞悬液以不同浓度基质细胞衍生因子1(1 μmol/L，100 nmol/L， 10 nmol/L，1 nmol/L，0.1 nmol/L，0 nmol/L)处理。
结果与结论：实验成功构建了CXCR4慢病毒表达载体。正常人支气管上皮细胞的CXCR4蛋白基础表达较低。转染CXCR4的人支气管上皮细胞中CXCR4 mRNA和蛋白都有较高水平的表达，且随着基质细胞衍生因子1浓度增加，其吸光度逐渐增加，并呈浓度依赖性(P < 0.05)。提示基质细胞衍生因子1增强了转染其受体CXCR4基因支气管上皮细胞的增殖作用和定向迁移能力。

关键词: 基质细胞衍生因子1, 趋化因子, 支气管上皮细胞, 基因, 转染

Abstract:

BACKGROUND: A key problem of study on tracheal prosthesis is to cover the tracheal mucosa and recover its function. Stromal cell derived factor-1/CXCR4 pathway plays an important role in accelerating tissue repair.
OBJECTIVE: To investigate the effects of stromal cell derived factor-1 on the proliferation and directional migration of bronchial epithelial cells transfected by CXCR4 gene.
METHODS: CXCR4 lentiviral vector was constructed and transfected into human bronchial epithelial cells. In the blank group, cells were not infected with any virus. In the control group, cells were infected with non-transfected lentiviral vectors. In the experimental group, cells were infected with CXCR4 lentiviral vectors. CXCR4 lentiviral vectors-transfected and common human bronchial epithelial cells were processed with different concentrations of stromal cell derived factor-1 (1 μmol/L, 100, 10, 1, 0.1, 0 nmol/L).
RESULTS AND CONCLUSION: CXCR4 lentiviral vector was successfully constructed. CXCR4 protein expression was low in the normal human bronchial epithelial cells. CXCR4 mRNA and protein expression was high in the CXCR4 gene- transfected human bronchial epithelial cells. With increasing concentration of stromal cell derived factor-1, absorbance was gradually increased in a concentration-dependent manner (P < 0.05). This suggests that stromal cell derived factor-1 promotes the proliferation and directional migration of bronchial epithelial cells transfected by CXCR4 gene.

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