中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4663-4666.doi: 10.3969/j.issn.1673-8225.2012.25.021

• 药物控释材料 drug delivery materials • 上一篇    下一篇

成骨生长肽壳聚糖-海藻酸钠微球的制备及体外检测

马树强1，彭建强1，李中檀1，李玉红1，巫伟东1，林奇生1，王坤正2   

  1. 1深圳市第四人民医院骨科，广东省深圳市 518033；
    2西安交通大学第二医院骨科，陕西省西安市 710004
  • 收稿日期:2011-11-11 修回日期:2011-12-20 出版日期:2012-06-17 发布日期:2013-11-04
  • 作者简介:马树强☆，男，1969年生，山东省日照市人，汉族，2008年西安交通大学医学院毕业，博士，副主任医师，主要从事骨关节外科，组织工程的研究。 mashuqiang369@163.com
  • 基金资助:

    广东省科技计划项目(2010A030400005)；深圳市科技局基金项目(201003221)；深圳市福田区科技局基金(FTWS201012)

Preparation and in vitro detection of osteogenic growth peptide chitosan-alginate microspheres

Ma Shu-qiang1, Peng Jian-qiang1, Li Zhong-tan1, Li Yu-hong1, Wu Wei-dong1, Lin Qi-sheng1, Wang Kun-zheng2   

  1. 1Department of Orthopedics, the Fourth People’s Hospital of Shenzhen, Shenzhen 518033, Guangdong Province, China;
    2Department of Orthopedics, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2011-11-11 Revised:2011-12-20 Online:2012-06-17 Published:2013-11-04
  • About author:Ma Shu-qiang☆, Doctor, Associate chief physician, Department of Orthopedics, the Fourth People’s Hospital of Shenzhen, Shenzhen 518033, Guangdong Province, China mashuqiang369@ 163.com

PDF

623

被引次数

13

摘要：

背景：成骨生长肽体外注射可以刺激外周血和骨髓细胞数增加，增加动物的骨量，加速骨折愈合，但因多肽不稳定性及注射应用不方便，限制了其临床应用。
目的：应用乳化交联法制备成骨生长肽壳聚糖-海藻酸钠缓释微球，并对其粒径、载药、体外释药、理化特性进行检测。
方法：以戊二醛作为交联剂，应用乳化交联法制备具有控制释放功能的负载成骨生长肽壳聚糖-海藻酸钠微球，显微镜及扫描电镜观察微球的形态和粒径；利用酶联免疫吸附实验动态检测成骨生长肽壳聚糖-海藻酸钠微球的载药率、包封率和缓释规律。
结果与结论：乳化交联法制备的壳聚糖-海藻酸钠微球，球形良好，球体表面有较多微孔，具有较高的包封率(>72%)。体外药物释放实验表明，成骨生长肽可以从壳聚糖-海藻酸钠微球中缓慢释放，整个释放过程可达49 d，累积释放率>85%。提示应用乳化交联法制备的负载成骨生长肽壳聚糖-海藻酸钠缓释微球，具有很好的控制释放成骨生长肽的能力。

关键词: 成骨生长肽, 缓释, 微球, 壳聚糖, 海藻酸钠, 生物材料

Abstract:

BACKGROUND: In vitro injection of osteogenic growth peptide (OGP) can regulate the peripheral blood and proliferation of bone marrow cells to increase bone formation and accelerate fracture healing, but it can not be worldly used for its instability and inconvenience of injection.
OBJECTIVE: To prepare the OGP chitosan-alginate microsphere by emulsion crosslinking, and to detect its particle size, drug loading, in vitro release, physical and chemical properties.
METHODS: The OGP loaded chitosan-alginic acid microsphere was prepared using an emulsion cross-linking method with glutaral as the cross-linking agent. Microscopy and scanning electron microscopy were used to evaluate the morphological properties and particle size distribution. The drug loading, entrapment efficiency and in vitro drug release kinetics were detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The OGP loaded chitosan-alginic acid microspheres were spherical in shape, and showed a asperity surface with many micropores and a higher encapsulation efficiency (> 72%). From the in vitro release experiment, the OGP was controlled to release from the chitosan microsphere over 49 days and the release property of microspheres in vitro was well and the overall release rate was > 85%. It is indicated that the OGP loaded chitosan-alginic acid microsphere that is prepared by emulsion cross-linking method shows a good controlled release property.

中图分类号: