中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (40): 7501-7504.doi: 10.3969/j.issn.1673-8225.2010.40.020

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

嗅鞘细胞与神经干细胞共培养后增殖及向神经元的分化

王秀娇1，盛宝英2，黄作义2，张晓梅2，魏春杰2   

  1. 1佳木斯大学神经病学系，黑龙江省佳木斯市 154002；2佳木斯大学附属第一医院神经三科，黑龙江省佳木斯市  154002
  • 出版日期:2010-10-01 发布日期:2010-10-01
  • 通讯作者: 盛宝英，硕士生导师，主任医师，佳木斯大学附属第一医院神经三科，黑龙江省佳木斯市 154002 SBY-N3@sohu.com
  • 作者简介:王秀娇★，女，1976年生，吉林省磐石县人，汉族，佳木斯大学在读硕士，主要从事神经干细胞方面的研究。 wxj9658@sohu.com
  • 基金资助:

    黑龙江省自然科学基金项目(D 2008-64)。

Proliferation and differentiation from neural stem cells into neurons following coculture with olfactory ensheathing cells

Wang Xiu-jiao1, Sheng Bao-ying2, Huang Zuo-yi2, Zhang Xiao-mei2, Wei Chun-Jie2   

  1. 1 Department of Neurology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China; 2 Third Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi  154002 Heilongjiang Province, China
  • Online:2010-10-01 Published:2010-10-01
  • Contact: Sheng Bao-ying, Master’s supervisor, Chief physician, Third Department of Neurology, First Affiliated Hospital, Jiamusi University, Jiamusi 154002 Heilongjiang Province, China SBY-N3@sohu.com
  • About author:Wang Xiu-jiao★, Studying for master’s degree, Department of Neurology, Jiamusi University, Jiamusi 154002, Heilongjiang Province, China wxj9658@sohu.com
  • Supported by:

     the Natural Science Foundation of Heilongjing Province, No.D2008-64*

PDF

446

被引次数

1

摘要：

背景：影响神经干细胞增殖分化的外在因素包括细胞因子和微环境，嗅鞘细胞能分泌多种细胞因子，与神经干细胞共培养时改变其微环境。
目的：观察不同浓度嗅鞘细胞对神经干细胞增殖及分化的影响。
方法：体外分别培养Wistar大鼠神经干细胞和嗅鞘细胞，5×107 L-1神经干细胞分别和1×107 L-1，1×109 L-1，1×1011 L-1嗅鞘细胞共培养，同时设立正常对照组，不加嗅鞘细胞。倒置荧光显微镜下观察神经干细胞增殖情况，诱导7 d后行NSE免疫细胞化学染色，计算阳性细胞/细胞总数得出阳性细胞百分比。
结果与结论： ①3种浓度嗅鞘细胞与神经干细胞共培养3 d后，均促进了神经干细胞增殖，以1×109 L-1嗅鞘细胞共培养组作用最显著，明显优于1×107 L-1嗅鞘细胞组、1×1011 L-1嗅鞘细胞组。②神经干细胞与1×109 L-1嗅鞘细胞共培养7 d后，神经干细胞分化为神经元样细胞的百分比最高，与正常对照组相比差异有显著性意义(P < 0.01)。

关键词: 嗅鞘细胞, 神经干细胞, 共培养, 增殖, 分化, 微环境

Abstract:

BACKGROUND: External factors that effect proliferation and differentiation of neural stem cells (NSCs) contain cytokines and microenviroment. Olfactory ensheathing cells (OECs) secrete a variety of cytokines and change the microenvironment of NSCs.
OBJECTIVE: To investigate effects of different concentrations of OECs on the proliferation and differentiation of NSCs.
METHODS: NSCs and OECs isolated from Wistar rats were cultured in vitro. 5×107/L NSCs were separately cocultured with 1×107/L, 1×109/L, 1×1011/L OECs. Normal control group was set up, without OECs. The proliferation of NSCs was observed under an inverted fluorescent microscope. At 7 days after co-culture, the expression of neuron specific enolase was detected by immunocytochemistry. Ratio of positive cells to total cells was calculated.
RESULTS AND CONCLUSION: At 3 days after co-culture, three different concentrations of OECs promoted the proliferation of NSCs. The effects were significant in the NSCs + 1×109/L OECs group, compared with NSCs + 1×107/L OECs group and NSCs + 1×1011/L OECs group. At 7 days after co-culture, the percentage of neurons immunostaining cells was significantly greater in the NSCs + 1×109/L OECs group, which showed significant differences compared with normal control group (P < 0.01).

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