中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9957-9959.doi: 10.3969/j.issn.1673-8225.2010.53.019

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

肾移植术后尿液DYZ-1检测的临床意义

周文强1, 2，石炳毅2，蔡  明2，钱叶勇2，李海斌2，肖  漓2，韩  永2，武洪文2   

  1. 1解放军军事医学科学院研究生大队，北京市   100850；2解放军第三○九医院器官移植中心研究室，北京市   100091
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 石炳毅，主任医师，博士生导师，解放军总医院第二附属医院器官移植中心，北京市 100091 shibingyi@medmail.com.cn
  • 作者简介:周文强★，1976年生，辽宁省新民市人，汉族，2010年解放军军事医学科学院毕业，硕士，主管技师，主要从事移植免疫学研究。 wenqiangzhou2050@sina.com
  • 基金资助:

    全军“十一五”计划医学科技攻关项目(06G115)。

Clinical significance of urinary DYZ-1 following renal transplantation

Zhou Wen-qiang 1,2, Shi Bing-yi2, Cai Ming2, Qian Ye-yong2, Li Hai-bin2, Xiao Li2, Han Yong2, Wu Hong-wen2   

  1. 1 Post-graduated Team, Academy of Military Medical Science, Beijing 100850, China; 2 Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Shi Bing-yi, Chief physician, Doctoral Supervisor, Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China shibingyi@medmail.com.cn
  • About author:Zhou Wen-qiang★, Master, Technician-in- charge, Post-graduated Team, Academy of Military Medical Science, Beijing 100850, China; Central Laboratory of Organ Transplantation, the 309 Hospital of Chinese PLA, Beijing 100091, China wenqiangzhou2050@sina.com
  • Supported by:

    the Medical Science and Technology Development Program during the Eleventh Five-Year Plan, No. 06G115*

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摘要：

背景：对移植受者尿液成分进行定期监测可以作为判断移植肾功能、状态的一种方法。
目的：分析肾移植受者尿液中供者细胞出现与急性排斥反应的关系及临床意义。
方法：选取60例供者为男性、受者为女性的肾移植受者，分为移植后早期检测组40例、急性排斥组10例、移植后功能稳定组10例。定期提取尿液中细胞DNA，利用PCR检测Y染色体上特异的基因片段DYZ-1。
结果与结论：在手术第1日受者的尿液中可检测到供者细胞DNA，随着时间的延长，尿液中供者细胞DNA量逐渐减少，至术后1个月，有8例受者的尿液中供者细胞DNA消失，其中1例发生急性排斥反应；另外32例受者的尿液中仍有供者细胞DNA的出现，其中7例发生了急性排斥反应；存活3个月以上发生急性排斥反应10例患者，7例尿液标本中能检测到供者细胞DNA，对急性排斥者进行治疗后1个月，71.4%转为阴性；而在稳定期的10例肾功能良好受者，仅1例受者尿液中DYZ-1基因阳性。提示对肾移植受者尿中供者细胞DNA的进行定期检测，可以作为监测急性排斥反应发生及预后的一种手段。

关键词: 急性排斥反应, 肾移植, 尿细胞, DNA, DYZ-1基因

Abstract:

BACKGROUND: Regular monitoring of recipient urine can be used to identify function and status of renal transplant.
OBJECTIVE: To investigate the relation and clinical significance of urinary donor-derived DNA in acute rejection after kidney transplantation.
METHODS: A total of 60 female renal transplantation recipients and male donors were selected and divided into early detection group (n=40), acute rejection group (n=10), and stable function group (n=10). Urine samples were collected regularly. PCR was applied to detect DYZ-1 (special gene fragment of Y-chromosome).
RESULTS AND CONCLUSION: Donor cells were detected in urine of all the recipients on day 1 after operation. With increasing time, the intensity of donor DNA expression in urine was decreased generally. At 1 month, donor cells in urine disappeared only in 8 of 40 cases, and acute rejection happened in 1 case; in other 32 recipients, donor cells remained in urine, and 7 cases developed acute rejection; in 10 cases of acute rejection after transplantation over 3 months, 7 cases presented the expression of donor cells in urine, and 1 month following anti-rejection therapy, donor cells in urine were negative in 71.4 % cases. In 10 cases of sable renal function, only 1 case had positive expression of DYZ-1. Results show that detection of urinary DNA of donor cells could be a method for diagnosing acute renal rejection of long term recipients.

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