中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (11): 2000-2003.doi: 10.3969/j.issn.1673-8225.2010.11.025

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

秋冬季节对健康大鼠肺组织表面活性蛋白A和白细胞介素6表达的影响

卢志刚1，王亚利1，2，张明泉1，郭锦桥1，师旭亮1，高 阳1   

  1. 1 河北医科大学中医学院中医基础教研室，河北省石家庄市  050091；
    2河北工程大学基础医学院，河北省邯郸市  056038
  • 出版日期:2010-03-12 发布日期:2010-03-12
  • 通讯作者: 王亚利，教授，主任医师，博士生导师，河北医科大学中医学院中医基础教研室，河北省石家庄市 050091 wang1955@163.com
  • 作者简介:卢志刚，男，1975年生，湖北省荆门市人，汉族，河北医科大学在读博士，主要从事中西医结合基础与临床研究。 luzg7501@sina.com
  • 基金资助:

    国家中医药管理局科研基金项目(06-07JP09)。

Effect of autumn and winter seasons on expression of surfactant associated protein A and interleukin-6 in healthy rat lung tissues

Lu Zhi-gang1, Wang Ya-li1,2, Zhang Ming-quan1, Guo Jin-qiao1, Shi Xu-liang1, Gao Yang1   

  1. 1School of Traditional Chinese Medicine, Hebei Medical University, Shijiazhuang  050017, Hebei Province, China;
    2Medical College, Hebei University of Engineering, Handan  056038, Hebei Province, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Wang Ya-li, Professor, Chief physician, Doctoral supervisor, School of Traditional Chinese Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China; Medical College, Hebei University of Engineering, Handan 056038, Hebei Province, China wang1955@163.com
  • About author:Lu Zhi-gang, Studying for doctorate, School of Traditional Chinese Medicine, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China luzg7501@sina.com
  • Supported by:

    the National Traditional Chinese Medicine Administration Foundation of China, No. 06-07JP09*

PDF

524

被引次数

11

摘要：

背景：近年来研究证实，季节气候的变化和肺表面活性物质蛋白A及白细胞介素6在肺脏免疫防御功能调节方面均起到很重要的作用，但是季节气候产生影响的机制目前还不清楚。
目的：观察秋冬季大鼠肺组织表面活性蛋白A和白细胞介素6的表达，认识季节和气候变化影响大鼠肺脏非特异性免疫的作用机制，为呼吸系统季节性发病的病理生理机制的认识提供实验依据。
方法：分别于立秋、秋分、立冬、冬至节气前14 d购入雄性SD实验大鼠，并分为相应组别。自由摄取水及正常饲料和室温饲养，接受自然光照。到相应节气，于当日下午6时将动物断头处死，取出全肺组织作表面活性蛋白A mRNA和白细胞介素6 mRNA检测。
结果与结论：与立秋、秋分、冬至组大鼠相比，立冬大鼠肺组织表面活性蛋白A和白细胞介素6均表达量较低，提示肺部免疫防御功能存在秋季高冬季低的季节节律，而表面活性蛋白A和白细胞介素6正是免疫防疫功能的物质基础。

关键词: 秋冬季节, 肺组织, 肺表面活性物质相关蛋白A, 白细胞介素6, 心肺组织工程

Abstract:

BACKGROUND: Previous study has confirmed that seasonal variation and surfactant associated protein A (SP-A) and interleukin-6 (IL-6) exhibit a significant role in lung immune and defense function. However, the effect mechanism of them remains unclear.
OBJECTIVE: By observing the changes of SP-A and IL-6 of male rats in autumn and winter, to explore the effects of seasonal changes on the nonspecific immunity of lung of normal rats, and to provide new ideas for the experimental basis to the cognition of pathology and physiology mechanism for seasonal attack of respiratory system diseases.
METHODS: Male SD rats were purchased from each seasons and divided into groups in chronological order. All rats were housed with normal forage and drank freely under room temperature. Then the rats were sacrificed by decapitation before 18 o’clock at corresponding solar term, and the lung tissues were analyzed by RT-PCR.
RESULTS AND CONCLUSION: Compared to the other groups, the expression of SP-A and IL-6 in the Winter Begins group were smaller, which demonstrated that immune function of lung was existed the seasonal rhythm of lower in winter and higher in autumn. The SP-A and IL-6 are main material basis of immune function.

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