中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5241-5245.doi: 10.3969/j.issn.2095-4344.2012.28.023

• 组织构建与生物力学 tissue construction and biomechanics • 上一篇    下一篇

p38丝裂素活化蛋白激酶与周期性张应力介导牙周膜成纤维细胞的增殖

曹海萌1，张广耘2，袁 晓2，尹崇英1   

  1. 1青岛大学医学院附属青岛市立医疗集团口腔科，山东省青岛市 266000；
    2青岛市市立医疗集团口腔科，山东省青岛市 266071
  • 收稿日期:2012-03-05 修回日期:2012-03-05 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 张广耘，主任医师，青岛市市立医院口腔科， 山东省青岛市 266071
  • 作者简介:曹海萌★，女，1986年生，山东省青岛市人，汉族，青岛大学医学院在读硕士，主要从事口腔内科牙周方向研究。 caohaimeng86@163.com

p38 mitogen-activated protein kinase effects on the proliferation of cyclic stretch-mediated periodontal ligament fibroblasts

Cao Hai-meng1, Zhang Guang-yun2, Yuan Xiao2, Yin Chong-ying1   

  1. 1Department of Stomatology, Qingdao Municipal Hospital (Oral), the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, Shandong Province, China;
    2Department of Stomatology, Qingdao Municipal Hospital (Oral), Qingdao 266071, Shandong Province, China
  • Received:2012-03-05 Revised:2012-03-05 Online:2012-07-08 Published:2012-07-08
  • Contact: Zhang Guang-yun, Chief physician, Department of Stomatology, Qingdao Municipal Hospital (Oral), Qingdao 266071, Shandong Province, China zhanggyqd@126. com
  • About author:Cao Hai-meng★, Studying for master’s degree, Department of Stomatology, Qingdao Municipal Hospital (Oral), the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, Shandong Province, China Caohaimeng86@163. com

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467

被引次数

4

摘要：

背景：体内环境的复杂多变，使得在体外研究机械应力对细胞的作用存在着一定难度。
目的：探讨p38丝裂素活化蛋白激酶信号通路在周期性张应力对人牙周膜成纤维细胞增殖中所产生的影响及其机制。
方法：构建人牙周膜成纤维细胞体外培养-力学刺激模型，利用多通道细胞牵张应力加载系统，分别对细胞加以1，6，12，24 h的周期性张应力，不加力组作为对照组，并在对照组和加力12 h组分别加入p38丝裂素活化蛋白激酶特异性抑制剂SB203580。细胞计数试剂8检测细胞增殖情况；RT-PCR检测细胞和增殖细胞核抗原的表达。
结果与结论：加力1h时细胞增殖得到促进，6 h时继续上升，12 h时达到高峰，24 h增殖受到抑制；SB203580可抑制细胞增殖和增殖细胞核抗原mRNA的表达。说明在一定时间范围内，周期性张应力可促进人牙周膜成纤维细胞增殖，但随时间延长，增殖受到抑制；p38丝裂素活化蛋白激酶信号通路在周期性张应力介导的人牙周膜成纤维细胞增殖中发挥重要作用。

关键词: 人牙周膜成纤维细胞, 张应力, 增殖, p38丝裂素活化蛋白激酶, 细胞计数试剂8

Abstract:

BACKGROUND: In vivo study of cellular mechanics obtains poor results due to complexity of physiological environment and uncontrollable experiment conditions.
OBJECTIVE: To study the cyclic stretch effects on human periodontal ligament fibroblasts proliferation and its mechanism as well as whether p38 mitogen-activated protein kinase signaling pathway participated in cell proliferation.
METHODS: In vitro culture-tensile stimulate models of human periodontal ligament fibroblasts were established by using a multi-passage load adding system. Control group was established without cyclic stretch. At the same time, cyclic stretch was applied on the fibroblasts for 1, 6, 12 and 24 hours. Cells in the control group and 12-hour load group were treated with p38 mitogen-activated protein kinase inhibitor SB203580 at 1 hour before loading. The cell proliferation was determined by cell counting kit-8 method, and the mRNA expression was detected on proliferating cell nuclear antigen by reverse transcription-PCR technology.
RESULTS AND CONCLUSION: Compared with the control group, the proliferation activity of human periodontal ligament fibroblasts was improved at 1 hour after loading, then increased at 6 hours and reached the peak at 12 hours; the proliferation of human periodontal ligament fibroblasts was inhibited obviously at 24 hours. SB203580 inhibited cell proliferation and inhibited mRNA expression of proliferating cell nuclear antigen. These results indicated, in a certain time, cyclic stretch can promote the proliferation of human periodontal ligament fibroblasts; as time went by, the proliferation of human periodontal ligament fibroblasts was obviously inhibited. p38 mitogen-activated protein kinase signaling pathway plays an important role during cyclic stretch-induced proliferation of human periodontal ligament fibroblasts.

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