中国组织工程研究

中国组织工程研究 ›› 2023, Vol. 27 ›› Issue (32): 5144-5149.doi: 10.12307/2023.592

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

柚皮苷对骨质疏松大鼠拔牙创骨愈合的影响

黄浩哲1，2，3，郑  敏1，2，3，李  娜1，2，3，孙黎波4，兰玉燕1，2，3，刘  敏1，2，3   

  1. 1西南医科大学附属口腔医院口腔修复科，四川省泸州市  646000；2口颌面修复重建和再生泸州市重点实验室，四川省泸州市  646000；3西南医科大学口腔医学研究所，四川省泸州市  646000；4四川省泸州市人民医院口腔科，四川省泸州市  646000
  • 收稿日期:2022-07-22 接受日期:2022-09-15 出版日期:2023-11-18 发布日期:2023-03-23
  • 通讯作者: 兰玉燕，硕士，讲师，西南医科大学附属口腔医院口腔修复科，四川省泸州市 646000；口颌面修复重建和再生泸州市重点实验室，四川省泸州市 646000；西南医科大学口腔医学研究所，四川省泸州市 646000
  • 作者简介:黄浩哲，1998年生，四川省达州市人，汉族，西南医科大学在读硕士，主要从事口腔科种植牙的基础研究工作。
  • 基金资助:
    四川省泸州市科技局[2017-S-42(1/2)]，项目负责人：孙黎波；四川省大学生创新创业训练计划项目(Q18011)，指导教师：兰玉燕；四川省大学生创新创业训练计划项目(S202210632150) ，指导教师：兰玉燕

Effects of naringin on healing of tooth extraction wound in osteoporotic rats

Huang Haozhe1, 2, 3, Zheng Min1, 2, 3, Li Na1, 2, 3, Sun Libo4, Lan Yuyan1, 2, 3, Liu Min1, 2, 3   

  1. 1Department of Prosthodontics, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; 3Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 4Department of Stomatology, Luzhou People’s Hospital, Luzhou 646000, Sichuan Province, China
  • Received:2022-07-22 Accepted:2022-09-15 Online:2023-11-18 Published:2023-03-23
  • Contact: Lan Yuyan, Master, Lecturer, Department of Prosthodontics, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Huang Haozhe, Master candidate, Department of Prosthodontics, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Luzhou 646000, Sichuan Province, China; Institute of Stomatology, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    Science and Technology Bureau of Luzhou City, No. 2017-S-42(1/2) (to SLB); Innovation and Entrepreneurship Training Program for College Students of Sichuan Province, No. Q18011 (to LYY [advisor]); Sichuan College Students Innovation and Entrepreneurship Training Program, No. S202210632150 (to LYY [advisor])

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摘要：


文题释义：
柚皮苷：是一种中药来源的单体化合物，主要存在于芸香科柑橘属植物的果皮和果肉中，具有抗炎和促成骨等作用。
OPG/RANKL/RANK：是调节骨代谢的重要信号通路之一。OPG和RANKL由成骨细胞表达，RANKL与破骨细胞表面RANK结合促进破骨细胞分化和成熟，OPG竞争性抑制RANKL与RANK结合。

背景：柚皮苷作为一种中药单体化合物，具有抗炎、促成骨的作用，但其对拔牙创愈合速度的影响并不明确。 
目的：建立骨质疏松大鼠拔牙模型，通过体内实验探究柚皮苷对拔牙创愈合速度的影响。 
方法：48只雌性SD大鼠随机分为假手术组14只与去势组34只。去势组去除双侧卵巢建立骨质疏松模型，6周后两组各随机取4只大鼠行MicroCT检测确认建模结果。建模成功后将剩余大鼠随机分为对照组、柚皮苷及雌二醇组，均拔除左上颌第二磨牙，柚皮苷组予以300 mg/(kg·d)柚皮苷灌胃，雌二醇组予以20 μg/(kg·d)17β雌二醇皮下注射，对照组予以等体积生理盐水处理。干预4周后，分离各组大鼠上颌骨进行苏木精-伊红染色、Masson染色、MicroCT检测，检测拔牙创周围骨组织骨保护素、核因子κB受体活化因子配体(receptor activator of nuclear factor-κB ligand，RANKL) mRNA及蛋白表达量。
结果与结论：①去势6周后，去势组骨体积分数降低，证明骨质疏松建模成功；②拔牙4周后，与对照组相比，柚皮苷组、雌二醇组骨小梁间距小、骨小梁数量多，牙槽骨高度丢失更少，说明柚皮苷可促进拔牙窝骨愈合；③与对照组相比，柚皮苷组、雌二醇组拔牙创周围骨组织骨保护素表达升高、RANKL表达降低，说明柚皮苷可以通过上调骨保护素和下调RANKL的表达水平，促进骨质疏松大鼠拔牙创骨愈合。
https://orcid.org/0000-0002-6871-7273(黄浩哲)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 骨质疏松症, 柚皮苷, 拔牙创, 骨愈合, OPG, RANKL, RANK, 大鼠

Abstract: BACKGROUND: Naringin, a monomer compound of Traditional Chinese medicine, has anti-inflammatory and osteogenic effects, but its effect on the healing rate of tooth extraction wounds is not clear.
OBJECTIVE: To investigate the effect of naringin on the healing rate of tooth extraction wounds in rat models of osteoporosis.
METHODS: Forty-eight female Sprague-Dawley rats were randomly divided into sham-operation group (n=14) and ovariectomized group (n=34). In the ovariectomized group, bilateral ovaries were removed to establish osteoporosis models. After 6 weeks, four rats in each group were randomly selected for MicroCT detection to confirm the modeling results. After successful modeling, the remaining rats were subject to tooth extraction and randomly divided into tooth extraction group (the remaining rats in the sham-operation group), control group, naringin group, and estradiol group, and the second molar of left maxillary was extracted. Naringin group was given naringin 300 mg/kg/d by gavage, estradiol group was given 17-β estradiol 20 μg/kg/d by subcutaneous injection, and tooth extraction group and control group were treated with the same volume of normal saline. After 4 weeks of intervention, the rat maxilla was separated for hematoxylin-eosin staining, Masson staining, and MicroCT detection. The expressions of osteoprotegerin and receptor activator of nuclear factor-κB ligand (RANKL) at mRNA and protein levels in the bone tissue around the tooth extraction wounds were also detected.  
RESULTS AND CONCLUSION: After 6 weeks of castration, the bone volume fraction of the ovariectomized group decreased, indicating that the modeling of osteoporosis was successful. Four weeks after tooth extraction, compared with the control group, the naringin group and estradiol group had smaller trabecular separation and less loss of alveolar bone height, indicating that naringin could promote bone healing of tooth extraction wounds. Compared with the control group, the expression level of osteoprotegerin and RANKL in bone tissues around tooth extraction was increased and decreased respectively in the naringin and estradiol groups, indicating that naringin could up-regulate osteoprotegerin expression and down-regulate RANKL expression.

Key words: osteoporosis, naringin, tooth extraction, bone healing, OPG, RANKL, RANK, rat

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