中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (7): 1030-1034.doi: 10.3969/j.issn.2095-4344.1065

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

化学神经根炎症模型大鼠的制备及评价

赵彩萍，朱美玲，王婷婷，柳雪蕾，刘翠玲   

  1. (广州中医药大学附属宝安中医院，广东省深圳市  518133)
  • 收稿日期:2018-09-25 出版日期:2019-03-08 发布日期:2019-03-08
  • 通讯作者: 朱美玲，博士，主任技师，博士生导师，广州中医药大学附属宝安中医院，广东省深圳市 518133
  • 作者简介:赵彩萍，女，汉族，山西省吕梁市人，1995年生，广州中医药大学在读硕士，主要从事临床检验诊断学方面的研究。
  • 基金资助:

    深圳市科技创新委员会基金资助项目(JCYJ20150401161033959)，项目负责人：朱美玲

A chemical radiculitis model in the rat: establishment and evaluation

Zhao Caiping, Zhu Meiling, Wang Tingting, Liu Xuelei, Liu Cuiling   

  1.  (Bao’an TCM Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen 518133, Guangdong Province, China)
  • Received:2018-09-25 Online:2019-03-08 Published:2019-03-08
  • Contact: Zhu Meiling, PhD, Chief technician, Doctoral supervisor, Bao’an TCM Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen 518133, Guangdong Province, China
  • About author:Zhao Caiping, Master candidate, Bao’an TCM Hospital Affiliated to Guangzhou University of Chinese Medicine, Shenzhen 518133, Guangdong Province, China
  • Supported by:

    the Funded Project of Shenzhen Municipal Science and Technology Innovation Committee, No. JCYJ20150401161033959 (to ZML)

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摘要：

文章快速阅读：

文题释义：
神经根炎：多种原因所致的神经根的炎性或变性病变。病变可侵及颈、胸、腰、骶任一节段的脊神经根，临床上以颈胸神经根和腰骶神经最常受累，可引起肩背痛及腰腿痛。
化学性神经根炎：在某些化学性因素的作用下，引起纤维环破裂，部分髓核液漏至椎间盘外并沿着神经根扩散引起神经根的一种炎症状态，常发生在L4、L5部位，如使用1%，2%，4%等一定浓度的甲醛溶液浸泡滤纸块后置于神经根旁，为一种常见的造成化学性神经根炎的方法。
摘要
背景：化学神经根造模实验优化为椎间盘的研究提供稳定的动物模型。
目的：探索甲醛浸泡滤纸诱发腰椎间盘突出-化学性神经根炎症大鼠模型构建方法，寻找最佳手术造模方案及造模浓度。
方法：先将10只由北京市华阜康生物科技股份有限公司提供的SD大鼠随机分为2组，分别采用咬断棘突及不咬断棘突后，在背根神经加以放置预浸泡4%甲醛滤纸进行造模，并结合行为学观察，确定最适手术造模方案。然后探索不同浓度甲醛溶液浸泡滤纸对造模的影响。将另20只SD大鼠随机分为4组，其中3组15只SD大鼠采用上述确定的手术方案(即咬断棘突横突方案)，分别在背根神经放置预浸泡1%，2%，4%甲醛溶液滤纸造模，并以Siegal神经评分、苏木精-伊红染色及相关炎症因子的检测结果综合评价造模效果。
结果与结论：与不咬断棘突的大鼠相比，咬断棘突L4、L5的大鼠Siegal神经评分及评级明显升高。与正常对照组相比，1%，2%，4%甲醛组大鼠在造模后2-8 d时Siegal神经评分及评级明显升高，但仅有2%和4%甲醛组的效果可以维持到2周时，且大鼠神经、肌肉、椎间盘组织内均出现较明显的炎症细胞浸润，血清相关炎症因子水平增高明显。提示改进后的模型制备方法，成模率高、死亡率低、模型稳定，可为后续疾病研究及药物研发提供理想的动物模型。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-8595-0579(朱美玲)

关键词: 化学神经根炎, 动物模型, 椎间盘, 大鼠, 炎症因子, 神经功能评分, 滤纸, 甲醛, 组织构建

Abstract:

BACKGROUND: Chemical nerve root modeling experiments provide a stable animal model for studying the intervertebral discs. 
OBJECTIVE: To explore the establishment of lumbar disc herniation and chemical nerve root inflammation in rats by formaldehyde soaked filter paper, and to find the best operation plan and the solution concentration.
METHODS: Ten Sprague-Dawley rats provided by Beijing Huafukang Bioscience Co., Inc., in China were randomly divided into two groups and subjected to biting off the spinous process or not. Then, 4% formaldehyde soaked filter papers were placed beside the dorsal root nerve to make the model. Based on behavioral performance, the optimal surgical plan was determined. Thereafter, the effect of different concentrations of formaldehyde soaked filter papers on the modeling. Another 20 Sprague-Dawley rats were randomized into 4 groups, 15 of which were subjected to the above-determined surgical plan of biting off the spinous process, and 1%, 2% and 4% formaldehyde soaked filter papers were placed beside the dorsal root nerve. Siegal neurological score, hematoxylin-eosin staining, and measurement of related inflammatory factors were used to assess modeling effects.
RESULTS AND CONCLUSION: The Siegal neurological score and grading were significantly increased in the rats with biting off the L4, 5 than those without biting off the spinous process. Compared with the control group, the Siegal neurological score and grading were markedly increased in 1%, 2%, 4% formaldehyde groups at 2-8 days after modeling, but this increasing tendency remained only in the 2% and 4% formaldehyde groups within 2 weeks after modeling. Moreover, significant inflammatory cell infiltrations occurred in the rat’s nerve, muscle and intervertebral disc, and the levels of serum related inflammatory factors increased remarkably. These findings indicate that the improved modeling method has high successful rate and low mortality, which can provide ideal animal models for subsequent diseases and drug researches.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Formaldehyde, Radiculopathy, Tissue Engineering

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