中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (6): 883-887.doi: 10.3969/j.issn.2095-4344.1537

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

牙科烤瓷合金对金黄地鼠颊囊黏膜中凋亡相关蛋白表达的影响

陈 润1，2，邱冰妍3，江 磊1，2，潘 宇1，4，王颖卉1，2，程 辉1，2，4   

  1. 1福建医科大学附属口腔医院修复科，福建省福州市 350002；2福建医科大学口腔医学研究院，福建省福州市 350002；3福建省级机关医院口腔科，福建省福州市 350001；4福建医科大学口腔医学美学与生物力学研究中心，福建省福州市 350002
  • 收稿日期:2018-09-01 出版日期:2019-02-28 发布日期:2019-02-28
  • 通讯作者: 程辉，教授，主任医师，福建医科大学附属口腔医院口腔修复科，福建省福州市 350002
  • 作者简介:陈润，男，1979年生，福建省闽候县人，汉族，2008年福建医科大学毕业，硕士，副主任医师，主要从事口腔修复学和口腔材料学研究。
  • 基金资助:

    国家自然科学基金(81641158)，项目负责人：程辉；福建省自然科学基金(2016J01354)，项目负责人：程辉

Effect of dental ceramic alloys on the expression of apoptosis-related signal proteins in the oral buccal mucosa of golden hamsters

Chen Run1, 2, Qiu Bingyan3, Jiang Lei1, 2, Pan Yu1, 4, Wang Yinghui1, 2, Cheng Hui1, 2, 4   

  1. 1Department of Prosthodontics, Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China; 3Departmetn of Stomatology, Fujian Provincial Governmental Hospital, Fuzhou 350001, Fujian Province, China; 2Institute of Stomatology, 4Center for Stomatology Aesthetics and Biomechanics, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • Received:2018-09-01 Online:2019-02-28 Published:2019-02-28
  • Contact: Cheng Hui, Professor, Chief physician, Institute of Stomatology, Center for Stomatology Aesthetics and Biomechanics, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • About author:Chen Run, Master, Associate chief physician, Department of Prosthodontics, Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China; Institute of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81641158 (to CH); the Natural Science Foundation of Fujian Province, No. 2016J01354 (to CH)

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摘要：

文章快速阅读：

 

文题释义：
牙科烤瓷合金：用于制作金属烤瓷修复体的合金材料，以金属合金作为基底，将牙科陶瓷材料在高温下熔附于金属表面。这些合金材料包括镍铬合金、钴铬合金、纯钛烤瓷合金、钯基合金、金铂合金等。
Caspase家族：是凋亡信号通路上一组重要的信号分子，大致可分为3类：第一类为凋亡启动因子，是凋亡信号通路中的上游信号分子，包括Caspase-2、Caspase-8、Caspase-9、Caspase-10等，它们能在特定情况下活化，还能够识别和激活信号通路中位于其下游的信号分子；第二类为凋亡执行因子，在凋亡信号转导通路的下游，包括Caspase-3、Caspase-6和Caspase-7等，它们能够作用于一些特异性的底物，最终导致细胞凋亡；第三类为炎症介导因子，包括Caspase-1、Caspase-4、Caspase-5和Caspase-11等，这些分子能够介导炎症反应并在诱导细胞凋亡时起辅助作用。
 
 
背景：有研究证实，牙科烤瓷合金具有不同程度的细胞毒性，可导致细胞凋亡，但以往关于牙科烤瓷合金引起细胞凋亡的研究多采用体外实验，无法很好地模拟口腔环境。
目的：观察牙科烤瓷合金对金黄地鼠颊囊黏膜中凋亡相关蛋白表达的影响。
方法：取60-70 d龄雄性金黄地鼠36只(北京维通利华实验动物技术有限公司提供)，随机分6组，分别在颊囊黏膜表面缝合固定镍铬烤瓷合金、钴铬烤瓷合金、纯钛烤瓷合金、钯基烤瓷合金、金铂烤瓷合金及聚丙烯(阴性对照)。固定14，28 d，切取与试件接触的颊囊黏膜，Western Blot检测黏膜细胞中Caspase-3、Caspase-8、Caspase-9蛋白表达情况。

结果与结论：①固定14，28 d时，金铂烤瓷合金组Caspase-3蛋白表达量与阴性对照组比较无明显差异(P > 0.05)，其余4种烤瓷合金组Caspase-3蛋白表达量高于阴性对照组(P < 0.05)；②固定14 d时，各烤瓷合金组Caspase-8蛋白表达量均高于阴性对照组(P < 0.05)；固定28 d时，镍铬烤瓷合金组、钯基烤瓷合金组Caspase-8蛋白表达量高于阴性对照组(P < 0.05)，其余烤瓷合金组Caspase-3蛋白表达量与阴性对照组比较无明显差异(P > 0.05)；③固定14 d时，各烤瓷合金组Caspase-9蛋白表达量均高于阴性对照组(P < 0.05)；固定28 d时，钴铬合金组Caspase-9蛋白表达量与阴性对照组比较无明显差异(P > 0.05)，其余烤瓷合金组Caspase-9蛋白表达量均高于阴性对照组(P < 0.05)；④结果表明，5种牙科烤瓷合金均不同程度引起凋亡相关信号分子的表达增加。

ORCID: 0000-0002-2601-1205(陈润)

关键词: 口腔材料, 牙科烤瓷合金, 细胞凋亡, 金黄地鼠, 颊囊黏膜, 凋亡相关蛋白

Abstract:

BACKGROUND: Dental porcelain alloys hold different levels of cytotoxicity, which can lead to cell apoptosis. However, the in vitro researches on dental ceramic alloy causing apoptosis are mainly conducted, in which the oral environment cannot be simulated well.

OBJECTIVE: To investigate the effects of dental ceramic alloys on apoptotic protein expression in the oral buccal mucosa of golden hamsters.
METHODS: Thirty-six 60-70-day-old male golden hamsters provided by Beijing Vital River Laboratory Animal Technology Co., Ltd. were randomly divided into six groups. Five kinds of dental ceramic alloys (nickel-chromium, cobalt chromium, CPTi, palladium based and gold-platinum alloys) and polypropylene (negative control) were fixed on the oral mucosa of golden hamsters in corresponding groups. The mucosae contacting with the specimens were removed at 14 and 28 days, respectively. The protein expression levels of Caspase-3, Caspase-8, and Caspase-9 in the buccal mucosa were detected by western blot assay.
RESULTS AND CONCLUSION: (1) At 14 and 28 days of fixation, there was no significant difference in the Caspase-3 protein level between gold-platinum alloy group and negative control groups (P > 0.05), and the level of Caspase-3 protein in the remaining four groups was significantly higher than that in the negative control group (P < 0.05). (2) At 14 days, the Caspase-8 protein level in each group was significantly higher than that in the negative control group (P < 0.05). After 28 days of fixation, the protein level of Caspase-8 in the nickel-chromium and palladium group was higher than that in the negative control group (P < 0.05), while the protein level of Caspase-8 in the other groups was not significantly different from that in the negative control group (P > 0.05). (3) At 14 days, the Caspase-9 protein level in each group was higher than that in the negative control group (P < 0.05). At 28 days, there was no significant difference in the Caspase-9 protein level between cobalt-chromium and negative control groups (P > 0.05), while the Caspase-9 protein level in the remaining groups was higher than that in the negative control group (P < 0.05). These results indicate that the use of five dental alloys can upregulate the levels of apoptosis-related signal molecules to different degrees.  

Key words: Metal Ceramic Alloys, Apoptosis, Dipodomys, Tissue Engineering

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