中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (21): 3417-3423.doi: 10.3969/j.issn.2095-4344.0906

• 干细胞综述 stem cell review • 上一篇    下一篇

Ⅰ型胶原诱导骨髓间充质干细胞及成骨细胞的成骨分化机制

谢 玉,周 诺   

  1. 广西医科大学附属口腔医院口腔颌面外科,广西壮族自治区南宁市 530021
  • 修回日期:2018-05-21 出版日期:2018-07-28 发布日期:2018-07-28
  • 通讯作者: 周诺,博士,教授,广西医科大学附属口腔医院口腔颌面外科,广西壮族自治区南宁市 530021
  • 作者简介:谢玉,女,1992年生,广西壮族自治区桂林市人,瑶族,广西医科大学附属口腔医院在读硕士,主要从事牵张成骨机制的研究。
  • 基金资助:

    国家自然科学基金项目(8167040353)

Type I collagen induction of bone marrow mesenchymal stem cells and the mechanism of osteogenesis

Xie Yu, Zhou Nuo   

  1. Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Revised:2018-05-21 Online:2018-07-28 Published:2018-07-28
  • Contact: Zhou Nuo, M.D., Professor, Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Xie Yu, Master candidate, Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 8167040353

摘要:

文章快速阅读:

文题释义:
生物矿化:
是指在机体内由细胞参与的无机物在有机物上成核、生长、定向成形的生物活动过程,在结晶过程中受内环境中基因、蛋白、矿物离子等诸多因子的调控。生物矿化具体分为3个阶段:有机质模板的形成、有机质-无机质的界面分子识别、矿物结晶多级结构组装。
液相前驱体:其最先在碳酸钙矿化过程中被发现。阴离子溶液达到一定临界浓度时,介于液体与晶体之间的相体从溶液中析出,这种相体沉积并同性融合成矿物膜,再失去结合水过渡到固态的晶体相。

 

摘要
背景:Ⅰ
型胶原为骨组织细胞外基质的主要成分,多项研究表明其具有诱导成骨分化的能力,其在骨组织矿化过程中起着重要的作用,目前生物材料领域正致力研究替代Ⅰ型胶原的仿生材料。
目的:综述Ⅰ型胶原诱导成骨的机制,在生物矿化过程中的作用及在牵张成骨过程中的实时表达。
方法:检索CNKI数据库和 PubMed 数据库检索1990至2017年期间关于Ⅰ型胶原诱导成骨、牵张成骨Ⅰ型胶原表达及生物矿化的文章,检索词分别为“Ⅰ型胶原(Ⅰ型胶原蛋白),骨髓间充质干细胞,成骨细胞,牵张成骨,生物矿化”和“type I collagen,bone marrow mesenchymal stem cells,osteoblast,distraction osteogenesis,mineralization”,最后选择58篇文章纳入结果分析。
结果与结论:①Ⅰ型胶原诱导骨髓间充质干细胞及成骨细胞成骨分化主要通过胶原-整合素信号通道;②在牵张成骨早期Ⅰ型胶原分泌较少,中后期分泌较多,成为细胞外主要基质;③矿化机制目前主流2种学说:Ⅰ型胶原在生物矿化中起到模板支架作用,引导钙盐沉积、矿化;无定形磷酸钙无机质渗入胶原纤维之间,由非晶体状态转为晶体状态,形成定向排列晶体结构。

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0003-2232-3794(谢玉)

关键词: Ⅰ型胶原, 骨髓间充质干细胞, 成骨细胞, 牵张成骨, 生物矿化, 整合素, 干细胞, 国家自然科学基金

Abstract:

BACKGROUND: Type I collagen is the main component of the extracellular matrix of bone tissue. Numerous studies have shown that it has the ability to induce osteogenic differentiation, and plays an important role in the process of bone mineralization. Currently, bionic materials are being developed as an alternative to type I collagen in the field of biological materials.
OBJECTIVE: To review the mechanism of osteogenesis induced by type I collagen, the role of type I collagen in the biomineralization process, and the real-time expression of type I collagen during distraction osteogenesis.
METHODS: With the key words of “type I collagen, bone marrow mesenchymal stem cells, osteoblast, distraction osteogenesis, mineralization” in Chinese and English, we performed a computer-based search in CNKI and PubMed database for relevant articles published from 1990 to 2017. Finally, 58 eligible articles were included in result analysis.
RESULTS AND CONCLUSION: Type I collagen induces osteogenic differentiation of bone marrow mesenchymal stem cells and osteoblasts mainly through collagen-integrin signaling pathways. In the early stage of distraction osteogenesis, type I collagen is less secreted, but the secretion increases in the middle and later stages, eventually becoming the main component of extracellular matrix. There are two mainstream theories of mineralization mechanisms: (1) Type I collagen plays a role as a template scaffold in biomineralization, guiding the deposition and mineralization of calcium. (2) Amorphous calcium phosphate infiltrates between collagen fibers and changes from amorphous to crystalline state, forming an aligned crystal structure.

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Collagen Type I, Bone Marrow, Mesenchymal Stem Cells, Osteoblasts, Tissue Engineering

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