中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (6): 821-826.doi: 10.3969/j.issn.2095-4344.0053

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

羟基磷灰石体系透钙磷灰石骨水泥的理化性能

彭  磊，丁秀明，陈克伟，刘建莉，顾运涛，卞阳阳，孟珠龙，姚江陵，牟忠林
  

  1. 海南医学院第一附属医院创伤中心，海南省海口市  570100
  • 收稿日期:2017-09-14 出版日期:2018-02-28 发布日期:2018-02-28
  • 通讯作者: 彭磊，海南医学院第一附属医院创伤中心，海南省海口市 570100
  • 作者简介:彭磊，男，1975年生，山东省威海市人，博士，教授，主要从事骨材料的研究。
  • 基金资助:
    国家自然科学基金资助项目(81460339)；海南省科技厅资助项目(SF201416)；海南省自然科学基金资助项目(514216)；海南省科技合作专项资助项目(KJHZ2015-05)；海南省卫生厅重点项目课题项目(2013-06)

Physicochemical properties of hydroxyapatite/dicalcium phosphate dehydrate bone cement

Peng Lei, Ding Xiu-ming, Chen Ke-wei, Liu Jian-li, Gu Yun-tao, Bian Yang-yang, Meng Zhu-long, Yao Jiang-ling, Mu Zhong-lin
  

  1. Trauma Center, First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China
  • Received:2017-09-14 Online:2018-02-28 Published:2018-02-28
  • Contact: Peng Lei, Trauma Center, First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China
  • About author:Peng Lei, M.D., Professor, Trauma Center, First Affiliated Hospital of Hainan Medical University, Haikou 570100, Hainan Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81460339; the Funding Project of Hainan Provincial Science and Technology Department, No. SF201416; the Natural Science Foundation of Hainan Province, No. 514216; the Science and Technology Cooperation Special Project of Hainan Province, No. KJHZ2015-05; the Key Project of Hainan Provincial Health Department, No. 2013-06

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摘要：

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文题释义：
磷酸钙骨水泥：根据水化产物将其可分为透钙磷灰石骨水泥和磷灰石类骨水泥，透钙磷灰石骨水泥在生理环境下溶解度较高，并能促进新骨的生成；而磷灰石类骨水泥降解速度非常慢，一般被视为不可降解，故透钙磷灰石骨水泥受到更多的关注。
 
背景：传统的透钙磷灰石骨水泥是将β-磷酸三钙与一水磷酸二氢钙作为反应物，但将羟基磷灰石作为透钙磷灰石骨水泥反应前体的报道却很少。
目的：验证羟基磷灰石/一水磷酸二氢钙体系是否能够生成透钙磷灰石骨水泥，并分析其理化性能。
方法：采用湿化学沉淀法分别制备羟基磷灰石和β-磷酸三钙，再分别与一水磷酸二氢钙以适当比例均匀混合，加入适量固化液水，得到羟基磷灰石/透钙磷灰石骨水泥和β-磷酸三钙/透钙磷灰石骨水泥，应用X射线衍射分析两种材料的结构和组分，扫描电镜观察两种材料的表面形态，Instron 5567 型万能材料实验机测试两种材料的力学强度。将两种材料分别浸泡于模拟体液中，检测失重率；浸泡14 d后取出，再行X射线衍射和扫描电镜检测。
结果与结论：①X射线衍射分析证实，两种材料均为纯度较高的透钙磷灰石骨水泥；②扫描电镜显示，β-磷酸三钙/透钙磷灰石骨水泥晶体结构更加密集，形成的孔隙相对较少；羟基磷灰石/透钙磷灰石骨水泥的晶粒较为细小，结构较为松散，孔隙数量也较多，生成的板状晶体小于β-磷酸三钙/透钙磷灰石骨水泥晶体；③β-磷酸三钙/透钙磷灰石骨水泥的抗压强度高于羟基磷灰石/透钙磷灰石骨水泥(P < 0.05)；④浸泡于模拟体液中后，β-磷酸三钙/透钙磷灰石骨水泥不同时间点的失重率均低于羟基磷灰石/透钙磷灰石骨水泥(P < 0.05)；在模拟体液中浸泡14 d后，X射线衍射及扫描电镜证实，两种材料表面均有一层由球状颗粒堆簇而成的羟基磷灰石生成；⑤结果表明，羟基磷灰石/透钙磷灰石骨水泥具有良好的生物降解性能、生物活性及骨传导性，但力学性能较差。

关键词: 羟基磷灰石/透钙磷灰石, β-磷酸三钙/透钙磷灰石, 表面形态, 力学强度, 失重率, 生物材料, 国家自然科学基金

Abstract:

BACKGROUND: β-tricalcium phosphate (β-TCP) and monocalciumphosphate monohydrate (MCPM) are traditionally considered as reactants for dicalcium phosphate dehydrate (DCPD) bone cement, but little is reported on the hydroxyapatite (HA) as a reactant.
OBJECTIVE: To verify whether HA and MCPM can be used to prepare DCPD bone cement and to explore the physicochemical properties.
METHODS: The HA and β-TCP were prepared by wet chemical precipitation method, and mixed with appropriate proportion of MCPM. Then, the HA-DCPD and β-TCP-DCPD were obtained by adding a proper amount of curing water. The composition and structure of the two materials were analyzed by X-ray diffraction, the morphology was observed by scanning electron microscope, and the mechanical strength was tested by Instron5567 universal material test machine. These two kinds of materials were placed in simulated body fluid for detecting the weight loss ratio, soaked for 14 days and taken out for X-ray diffraction and scanning electron microscope detection.
RESULTS AND CONCLUSION: X-ray diffraction findings indicated that these two kinds of materials both belonged to high-purity DCPD bone cement. Under the scanning electron microscope, β-TCP-DCPD bone cement had dense crystal structure, with less pore number; however, the HA-DCPD bone cement presented with finer grains, loose structure, and higher pore number. With the increase of curing time, the mechanical strength of two kinds of bone cements was correspondingly increased, but the compressive strength of β-TCP-DCPD bone cement was significantly higher than that of HA-DCPD bone cement (P < 0.05). In the simulated body fluid, the weight loss ratio of β-TCP-DCPD bone cement was significantly lower than that of HA-DCPD bone cement (P < 0.05). At 14 days after soaking in the simulated body fluid, a layer of spherical particles that was formed on the surface of both materials was identified as hydroxyapatite by scanning electron microscope observation and X-ray diffraction analysis. In summary, HA-DCPD bone cement has good biodegradability, excellent bioactivity and bone conductivity, but poor mechanical properties.

Key words: Calcium Phosphates, Hydroxyapatites, Apatite, Tissue Engineering

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