中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (4): 530-537.doi: 10.3969/j.issn.2095-4344.2017.04.007

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

人牙周膜细胞与高糖损伤的代谢记忆效应

任伟伟1，李守宏2，熊  洁1，张  帆1，管  琴1   

  1. 湖北医药学院附属东风口腔医院，1预防保健科，2颌面外科，湖北省十堰市  442001
  • 收稿日期:2016-11-24 出版日期:2017-02-08 发布日期:2017-03-13
  • 通讯作者: 通讯作者：李守宏，博士，主任医师，教授。湖北医药学院附属东风口腔医院颌面外科，湖北省十堰市442001
  • 作者简介:任伟伟，男，1978年生，湖北省十堰市人，汉族， 2015年潍坊医学院毕业，硕士，主治医师。
  • 基金资助:

    湖北省教育厅青年人才项目(Q20162108)；十堰市科技局计划项目(15y50)

High glucose induces a metabolic memory in human periodontal ligament cells

Ren Wei-wei1, Li Shou-hong2, Xiong Jie1, Zhang Fan1, Guan Qin1   

  1. 1Department of Prevention Dentistry, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442001, Hubei Province, China; 2Department of Oral and Maxillofacial Surgery, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442001, Hubei Province, China
  • Received:2016-11-24 Online:2017-02-08 Published:2017-03-13
  • Contact: Corresponding author: Li Shou-hong, M.D., Chief physician, Professor, Department of Oral and Maxillofacial Surgery, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442001, Hubei Province, China
  • About author:Ren Wei-wei, Master, Attending physician, Department of Prevention Dentistry, Dongfeng Stomatological Hospital, Hubei University of Medicine, Shiyan 442001, Hubei Province, China
  • Supported by:

    the Young Talent Project of Hubei Provincial Department of Education, No. Q20162108; the Program of the Science and Technology Bureau of Shiyan, No. 15y50

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摘要：

文章快速阅读：

文题释义：
人牙周膜细胞：是参与牙周组织再生的最重要细胞之一，高糖对牙周膜细胞的增殖和凋亡，生物学活性，信号通路等均有影响。有研究发现人牙周膜细胞对高糖影响存在“代谢记忆”效应，具体表现在细胞增殖，凋亡，碱性磷酸酶活性，细胞总蛋白分泌等方面。
代谢记忆：指因为糖尿病患者血糖水平长时间较高，就会使靶细胞对早期的血糖环境产生记忆，后期即使血糖得以控制，靶细胞仍处于“高血糖”记忆状态，仍然不能降低糖尿病相关并发症的发病率。
摘要
背景：目前高糖影响人牙周膜细胞相关研究多集中在细胞生物学行为、相关通路、因子分泌研究等方面，但对于人牙周膜细胞对高糖影响是否存在“代谢记忆”效应的报道较少。
目的：观察人牙周膜细胞对高糖损伤是否存在记忆效应。
方法：原代培养并鉴定人牙周膜细胞，实验采用5-8代细胞，划分为对照组、5 d记忆组、3 d记忆组、8 d高糖组：对照组葡萄糖浓度为5.5 mmol/L DMEM培养液培养8 d；5 d记忆组用35 mmol/L DMEM培养液刺激3 d后5.5 mmol/L DMEM培养液培养5 d；3 d记忆组35 mmol/L DMEM培养液刺激5 d后5.5 mmol/L DMEM培养液培养3 d；8 d高糖组葡萄糖浓度35 mmol/ L DMEM培养液进行8 d的刺激。细胞增殖通过CCK-8法进行测定，细胞凋亡通过流式细胞仪进行检测，采用酶标仪检测总蛋白的合成及碱性磷酸酶水平变化。
结果与结论：与对照组比较，其他3组中的细胞增殖速度明显降低(P < 0.05)。细胞凋亡明显升高，总蛋白合成以及碱性磷酸酶分泌水平，所有高糖组总蛋白合成以及碱性磷酸酶分泌水平则明显下降(P < 0.05)。结果说明，人牙周膜细胞在高糖环境中产生的损伤具有记忆效应，以细胞活力长时间下降、凋亡增加、总蛋白合成、碱性磷酸酶水平持续下调为主要表现。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-4568-8921(李守宏)

关键词: 组织构建, 组织工程, 高糖, 人牙周膜细胞, 代谢记忆, 增殖, 凋亡, 流式细胞术, 总蛋白合成, 碱性磷酸酶, 细胞生物学活性

Abstract:

Abstract
BACKGROUND: Studies on high glucose exposure in human periodontal ligament cells usually focus on the biological behaviors, pathways and secretory factors, but whether the metabolic memory is involved is little known.
OBJECTIVE: To investigate the metabolic memory of high glucose exposure in human periodontal ligament cells. 
METHODS: Human periodontal ligament cells were primarily cultured and identified. Cells at 5-8 passages were selected and randomized into four groups. Group A (controls): DMEM containing 5.5 mmol/L glucose for 8 days; group B (5-day memory group): DMEM containing 35 mmol/L glucose for 3 days and DMEM containing 5.5 mmol/L glucose for 5 days; group C (3-day memory group): DMEM containing 35 mmol/L glucose for 5 days and DMEM containing 5.5 mmol/L glucose for 3 days; group D (8-day high glucose group): DMEM containing 35 mmol/L glucose for 8 days. The cell proliferation was detected by cell counting kit-8, the cell apoptosis was determined by flow cytometry, and the levels of total proteins and alkaline phosphatase were investigated using ELISA.
RESULTS AND CONCLUSION: Compared with the control group, the cell proliferation in the other three groups was significantly reduced (P < 0.05), the number of apoptotic cells was significantly increased, while the levels of total proteins and alkaline phosphatase were significantly decreased (P < 0.05). These results suggest that high glucose causes persistent changes in human periodontal ligament cells by inhibiting cell viability, increasing the apoptosis and downregulating the levels of the total proteins and alkaline phosphatase

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Apoptosis, Alkaline Phosphatase

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