中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (49): 7397-7403.doi: 10.3969/j.issn.2095-4344.2016.49.015

• 口腔损伤及修复动物模型 Animal models of oral lesions and repair • 上一篇    下一篇

Wistar大鼠咬合创伤模型颌骨与牙周毛细血管的铸型

毕振宇1，刘  阳2，武  斌2，谢  春2，杨宇超1，欧阳钧1   

  1. 1南方医科大学人体解剖学教研室，广东省医学生物力学重点实验室，广东省广州市  510515；2深圳市龙华新区人民医院口腔科，广东省深圳市  518109
  • 收稿日期:2016-09-03 出版日期:2016-11-30 发布日期:2016-11-30
  • 通讯作者: 欧阳钧，博士，教授，博士生导师，南方医科大学人体解剖学教研室，广东省医学生物力学重点实验室，广东省广州市 510515
  • 作者简介:毕振宇，男，1972年生，河南省洛阳市人，博士，讲师，主要从事数字解剖学与生物力学研究。
  • 基金资助:

    国家自然科学基金(31170903)；深圳市卫生计生系统科研项目(201506088)

Jaw bone and periodontal capillary casting in a Wistar rat model of occlusal trauma

Bi Zhen-yu1, Liu Yang2, Wu Bin2, Xie Chun2, Yang Yu-chao1, Ouyang Jun1   

  1. 1Department of Anatomy of Southern Medical University, Medical Biomechanics Key Laboratory of Guangdong Province, Guangzhou 510515, Guangdong Province, China; 2Department of Stamatology, People’s Hospital of Longhua New District of Shenzhen, Shenzhen 518109, Guangdong Province, China
  • Received:2016-09-03 Online:2016-11-30 Published:2016-11-30
  • Contact: Ouyang Jun, M.D., Professor, Doctoral supervisor, Department of Anatomy of Southern Medical University, Medical Biomechanics Key Laboratory of Guangdong Province, Guangzhou 510515, Guangdong Province, China
  • About author:Bi Zhen-yu, M.D., Lecturer, Department of Anatomy of Southern Medical University, Medical Biomechanics Key Laboratory of Guangdong Province, Guangzhou 510515, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31170903; the Project of Health and Family Planning Commission of Shenzhen, No. 201506088

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被引次数

1

摘要：

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文题释义：
毛细血管铸型的意义：显示体内状态下微血管的空间分布与毗邻关系，印证了组织学切片观察，更加直接显示毛细血管形态。随着灌注材料和设备改进，毛细血管铸型更加符合体内实际情况，同时也为其生物力学研究、生理生化研究、分子生物学研究、基因与靶向研究提供有力的解剖形态学依据。
咬合创伤：在临床中大致有2种因素类型，由于过大咬合力引发，称为原发性咬合创伤；其次由于牙周组织发生生理或者病理性改变，使其不能支持正常的咬合力，导致牙周组织再次损伤，称为继发性咬合创伤。
摘要
背景：咬合创伤被认为牙周疾病发生和发展的重要因素，目前咬合创伤的临床研究和基础研究也逐渐成为一个热点。
目的：探索咬合创伤对颌骨与牙周微血管的影响。
方法：取48只Wistar大鼠，随机选取6只作为正常对照，仅作麻醉处理；另42只在下颌右侧磨牙区域设置1 mm高点，模拟早接触形成咬合创伤模型，分别于造模后3 d、1周、2周、3周、4周、6周、8周进行牙周毛细血管铸型(每个时间点6只)，解剖分离出部分颌骨(包括牙、牙周组织)，扫描电镜下观察铸型标本。
结果与结论：3 d组和1周组标本炎症反应明显，血管网无明显减少，毛细血管迂曲变细，牙槽骨无明显吸收；2周、3周、4周组标本牙槽骨逐渐吸收明显，毛细血管管径变细，结构更加迂曲，密度明显降低；6周组标本牙槽骨吸收没有加重，毛细血管数量开始增加；8周组标本中毛细血管数量变多，管径变粗。结果表明，咬合创伤可导致牙周毛细血管形态学变化。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程
ORCID: 0000-0002-3726-7126(毕振宇)

关键词: 实验动物, 口腔损伤与修复动物模型, 毛细血管铸型, Wistar大鼠, 颌骨, 牙周, 微循环, 牙周病, 咬合创伤, 国家自然科学基金

Abstract:

BACKGROUND: Occlusal trauma, a key factor of occurrence and development of periodontal diseases, has gradually become a hot spot of basic and clinical research.
OBJECTIVE: To explore the effect of occlusal trauma on jaw bone and periodontal capillaries.
METHODS: Forty-eight Wistar rats were enrolled and six rats were randomly selected as control group given anesthesia only. The photo-curing material was set at the 1 mm high of right mandibular molar area in 42 rats to simulate early contact inducing occlusal trauma. Periodontal capillary casting was conducted at 3 days, 1, 2, 3, 4, 6 and 8 weeks after modeling (6 rats per time point); the part of the jaw including teeth and periodontal tissues were isolated, and the casting specimens were observed by scanning electron microscope.
RESULTS AND CONCLUSION: At 3 days and 1 week after modeling, overt inflammatory reaction, slight reduction of vascular networks, and tortuous and thinner capillaries could be found, and alveolar bones had not been absorbed obviously. At 2, 3, and 4 weeks, the alveolar bones were absorbed gradually, and the capillary diameter and density were decreased markedly becoming more tortuous. The jaw bone absorption remained stable with increased capillaries at 6 weeks. Up to 8 weeks, abundant thinner capillaries appeared. These findings suggest that occlusal trauma will give rise to the morphological changes of periodontal capillaries.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Rats, Wistar, Mandible, Capillaries, Periodontium

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