中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (37): 6005-6009.doi: 10.3969/j.issn.2095-4344.2015.37.019

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

透骨消痛胶囊含药血清对膝骨关节炎关节滑膜细胞uPA系统及炎性因子的影响

吴广文1，陈 俊2，叶锦霞1，郑春松1，刘伯龄3，刘献祥1，叶蕻芝1   

  1. 1福建中医药大学中西医结合研究院，福建省福州市  350122； 2福建中医药大学中西医结合学院，福建省福州市  350122； 3福建省福州市第二医院，福建省福州市  350007
  • 出版日期:2015-09-10 发布日期:2015-09-10
  • 通讯作者: 叶蕻芝，女，研究员，硕士生导师，福建中医药大学中西医结合研究院，福建省福州市 350122
  • 作者简介:吴广文，男，1982年生，汉族，2014年福建中医药大学毕业，博士，助理研究员，主要从事骨性关节炎研究。
  • 基金资助:

    国家自然科学基金资助项目(81202712)；福建省教育厅A类科技杰青项目(JA14151)

Effects of Tougu Xiaotong Capsule medicated serum on the expression of uPA system and infammatory factors in osteoarthritis synovial cells 

Wu Guang-wen1, Chen Jun2, Ye Jin-xia1, Zheng Chun-song1, Liu Bo-ling3, Liu Xian-xiang1, Ye Hong-zhi1   

  1. 1Academy of Integrated Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China; 2College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China; 3Department of Orthopedics, the Fuzhou No. 2 Hospital, Fuzhou 350007, Fuzhou Province, China
  • Online:2015-09-10 Published:2015-09-10
  • Contact: Ye Hong-zhi, Master’s supervisor, Researcher, Academy of Integrated Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • About author:Wu Guang-wen, M.D., Assistant researcher, Academy of Integrated Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81202712; the Grade A Scientific Young Talents Program of Fujian Provincial Education Department, No. JA14151

PDF

266

被引次数

4

摘要：

背景：透骨消痛胶囊是治疗骨性关节炎的临床验方，作用机制尚未完全阐明。尿激酶型纤溶酶原激活系统参与软骨细胞外基质降解和滑膜增生，在骨性关节炎发生、发展中起重要作用。

目的：观察透骨消痛胶囊含药血清对体外培养的膝骨性关节炎大鼠滑膜细胞表达尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、纤溶酶原激活剂抑制剂、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α的影响，探讨透骨消痛胶囊防治膝骨性关节炎的作用机制。

方法：采用4%木瓜蛋白酶注射关节腔复制大鼠膝骨性关节炎模型；胶原酶消化法原代培养正常滑膜细胞与骨性关节炎滑膜细胞，将所培养滑膜细胞分为正常组、模型组和透骨消痛胶囊组，前2组细胞用空白血清培养，透骨消炎胶囊用含药血清培养；透骨消痛胶囊含药血清作用骨性关节炎滑膜细胞72 h后，采用Western Blot法检测滑膜细胞中尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、纤溶酶原激活剂抑制剂、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α蛋白的表达。

结果与结论：透骨消痛胶囊含药血清组尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α蛋白表达量均降低，纤溶酶原激活剂抑制剂表达量升高，与模型组相比均有显著性差异，提示透骨消痛胶囊治疗骨性关节炎的部分作用机制是有效抑制骨性关节炎滑膜细胞尿激酶型纤溶酶原激活剂、尿激酶型纤溶酶原激活剂受体、基质金属蛋白酶3、白细胞介素1β和肿瘤坏死因子α的表达，提高纤溶酶原激活剂抑制剂的表达。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 骨关节炎, 滑膜, 尿激酶型纤溶酶原激活剂, 中草药

Abstract:

BACKGROUND: Tougu Xiaotong Capsule (TGXTC) is a clinical prescription for the treatment of osteoarthritis; however, its mechanism has not been fully elucidated. Urokinase-type plasminogen activator (uPA) system 
participating in the degradation of the extracellular matrix of articular cartilage and hyperplasia of joint synovium plays an important role in the pathological process of osteoarthritis.

OBJECTIVE: To investigate the effects of TGXTC medicated serum on the expression of uPA, uPA receptor (uPAR), plasminogen activator inhibitors (PAIs), matrix metalloproteinase-3 (MMP-3), interleukin-1 beta (IL-1β) and tumor necrosis factor-α (TNF-α) in osteoarthritis synovial cells of rats and to discuss the mechanism by TGXTC medicated serum prevents and cures osteoarthritis.

METHODS: Rat models with knee osteoarthritis were established by injecting 4% papain into the knee joint cavity. Primary synoviocytes and osteoarthritis synoviocytes were cultured with collagenase digestion method. The cultured synoviocytes were divided into normal group, model group and TGXTC group. The western blot method was adopted to detect uPA, uPAR, PAI, MMP-3, IL-1β and TNF-α protein expression of synoviocytes after acting by TGXTC medicated serum for 72 hours.

ESULTS AND CONCLUSION: The expression of uPA, uPAR, MMP-3, IL-1β and TNF-α were decreased, while PAI was increased in the TGXTC group, and there were significant differences when compared with model group. In a word, TGXTC can significantly inhibit the expression of uPA, uPAR, MMP-3, IL-1β, TNF-α, and improve PAI expression in synoviocytes, which may partly explain the mechanism of the treatment of Tougu Xiaotong Capsule on osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Synovial Membrane, Urokinase-Type Plasminogen Activator, Drugs, Chinese Herbal

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