中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (14): 2649-2655.doi: 10.3969/j.issn.2095-4344.2013.14.026

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

间充质干细胞的分离与培养:从实验室到临床

李炳尧1,武晓云2,吴  岩1   

  1. 1内蒙古医科大学,内蒙古自治区呼和浩特市  010059
    2北京京蒙高科干细胞技术有限公司,北京市  100085
  • 收稿日期:2012-06-08 修回日期:2012-08-27 出版日期:2013-04-02 发布日期:2013-04-02
  • 通讯作者: 吴岩,博士,教授,硕士生导师,内蒙古医科大学,内蒙古自治区呼和浩特市 010059 yanw007@sina.com
  • 作者简介:李炳尧★,男,1982年生,内蒙古自治区赤峰市人,汉族,内蒙古医科大学在读硕士,医师,主要从事间充质干细胞培养、临床应用研究。 cell2112@sina.com
  • 基金资助:

    内蒙古科技厅干细胞创新团队项目(KJT2009)。

Isolation and culture of mesenchymal stem cells: From laboratory research to clinical application  

Li Bing-yao1, Wu Xiao-yun2, Wu Yan1   

  1. 1 Inner Mongolia Medical University, Hohhot  010059, Inner Mongolia Autonomous Region, China
    2 Beijing Jingmeng Stem Cell High-Tech Co., Ltd., Beijing  100085, China
  • Received:2012-06-08 Revised:2012-08-27 Online:2013-04-02 Published:2013-04-02
  • Contact: Wu Yan, M.D., Professor, Master’s supervisor, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China yanw007@sina.com
  • About author:Li Bing-yao★, Studying for master’s degree, Physician, Inner Mongolia Medical University, Hohhot 010059, Inner Mongolia Autonomous Region, China cell2112@sina.com
  • Supported by:

    Stem Cell Innovation Team Program of Science and Technology Bureau of Inner Mongolia Autonomous Region of China, No. KJT2009

摘要:

背景:间充质干细胞是多能成体干细胞,对间充质干细胞来源、分离、培养的研究无论从观念上、研究方法上还是研究结果均不尽相同。
目的:综述间充质干细胞分离、培养研究的新进展。
方法:由作者应用计算机检索2007至2011年PubMed 数据库,在英文标题和摘要中以“mesenchymal stem cells”和“source, isolation or culture”检索,选择内容与间充质干细胞应用相关的文章,同一领域文献则选择近期发表或发表在权威杂志文章,共纳入43篇文献。
结果与结论:间充质干细胞的规范化培养需要建立一套标准化的培养体系来实现间充质干细胞的分离、大量增殖。因此,需要进一步深入开展间充质干细胞的基础研究,建立体外扩增和向不同细胞定向诱导分化体系,开展间充质干细胞及转基因后移植的体内效应研究等均具有重要的意义。

关键词: 干细胞, 干细胞学术探讨, 间充质干细胞, 来源, 分离, 非分化扩增, 无血清, 微载体悬浮培养, 表型, 分化, 脐带, 省级基金

Abstract:

BACKGROUND: Mesenchymal stem cells are multipotent adult stem cells. The source, isolation and culture of mesenchymal stem cells differ greatly in different studies in terms of concepts, research methods and outcomes.
OBJECTIVE: To investigate the progress in isolation and culture of mesenchymal stem cells.
METHODS: The PubMed database (http://www.ncbi.nim.nih.gov/PubMed) was retrieved for articles regarding application of stem cells from 2007 to 2011 by computer. The English key words are “mesenchymal stem cells” and “source isolation or culture”. The papers published recently or in authoritative journals were selected. Forty-three papers were suitable for final analysis.  
RESULTS AND CONCLUSION: A standardized culture system should be established to achieve isolation and expansion in vitro of mesenchymal stem cells. Therefore, basic research of mesenchymal stem cells should be further developed, and the system of in vitro expansion and oriented differentiation of mesenchymal stem cells should be established, and study on the effects of transplantation of gene-transfected mesenchymal stem cells is of great significance.

Key words: stem cells, stem cell academic discussion, mesenchymal stem cells, source, isolation, non-differentiation expansion, serum-free, microcarrier suspending culture, phenotype, differentiation, umbilical cord, provincial grants-supported paper

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