中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (29): 4593-4756.doi: 10.3969/j.issn.2095-4344.2015.29.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

葛根素可促进老年女性骨质疏松症患者成骨细胞的增殖

孙玉敏1，许晓琳2，杨  怡1，黄冬冬1，许金秀3，程  才3，王  路3   

  1. 沧州医学高等专科学校，1内科，2内护教研室，河北省沧州市  061001；3沧州市中心医院，河北省沧州市  061001
  • 出版日期:2015-07-09 发布日期:2015-07-09
  • 通讯作者: 孙玉敏，沧州医学高等专科学校内科，河北省沧州市 061001
  • 作者简介:孙玉敏，女，1975年生，河北省沧州市人，汉族， 2005年河北医科大学毕业，硕士，副教授、主治医师，主要从事内分泌方面的研究。
  • 基金资助:

    河北省科学技术研究与发展计划项目(12277752)；河北省教育厅资助科研项目(Z2012113)

Puerarin promotes the proliferation of osteoblasts in older female patients with osteoporosis  

Sun Yu-min1, Xu Xiao-lin2, Yang Yi1, Huang Dong-dong1, Xu Jin-xiu3, Cheng Cai3, Wang Lu3   

  1. 沧州医学高等专科学校，1内科，2内护教研室，河北省沧州市  061001；3沧州市中心医院，河北省沧州市  061001
  • Online:2015-07-09 Published:2015-07-09
  • Contact: Sun Yu-min, Department of Internal Medicine, Cangzhou Medical College, Cangzhou 061001, Hebei Province, China
  • About author:Sun Yu-min, Master, Associate professor, Attending physician, Department of Internal Medicine, Cangzhou Medical College, Cangzhou 061001, Hebei Province, China
  • Supported by:

    doi:10.3969/j.issn.2095-4344.2015.29.001

PDF

750

被引次数

37

摘要：

背景：近年来，葛根素作为植物雌激素其抗骨质疏松作用对实验动物及成骨细胞的研究较多，但对老年骨质疏松症患者成骨细胞作用的研究甚少，缺乏实验资料。
目的：观察葛根素对体外培养老年女性骨质疏松症患者成骨细胞增殖的影响。
方法：选择老年女性骨质疏松症股骨颈骨折行人工股骨头置换患者，采集术中取下的股骨颈松质骨，采用骨组织块培养法原代培养松质骨成骨细胞，将细胞传代至所需数量。对照组给予不含葛根素的培养液，0.01，0.1，1 μmol/L葛根素组分别给予含相应浓度葛根素的培养液。成骨细胞与不同浓度葛根素共培养1，3，5 d，观察成骨细胞增殖情况。
结果与结论：共培养不同时间点，0.01-1.00 μmol/L的葛根素随浓度增加，成骨细胞增殖活性不断增强(P < 0.05)；培养第3 天，各浓度葛根素组细胞吸光度值均最高。结果提示0.01，0.1，1 μmol/L的葛根素可呈浓度依赖性促进老年女性骨质疏松症患者成骨细胞增殖。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 骨组织工程, 葛根素, 骨质疏松, 骨折, 松质骨, 植物雌激素, 成骨细胞, 增殖, 组织工程, 碱性磷酸酶

Abstract:

BACKGROUND: In recent years, there are many animal studies and osteoblast studies on the anti-osteoporotic effects of puerarin, a kind of phytoestrogen. But few of them are reported on the effects of puerarin on osteoblasts in older patients with osteoporosis.
OBJECTIVE: To observe the influence of puerarin on the proliferation of in vitro cultured osteoblasts from older female patients with osteoporosis.
METHODS: The older female patients with osteoporotic femoral neck fractures who underwent artificial femoral head replacement were included in this study. The femoral neck cancellous bone removed during the operation was collected. Primary cancellous bone osteoblasts were cultured using explant culture method. The cells were sub-cultured to the required amounts. Osteoblasts from the control group were cultured with culture medium without puerarin. Osteoblasts from the 0.01, 0.1, and 1 μmol/L puerarin groups were cultured with culture medium containing the corresponding concentrations of puerarin. After in vitro co-culture with different concentrations of puerarin for 1, 3, 5 days, the proliferation of osteoblasts was observed.
RESULTS AND CONCLUSION: With the increase in the concentration of puerarin, the proliferative activity of
osteoblasts constantly increased at different time points (P < 0.05). At 3 days of culture, cell absorbance value in each group reached the peak level. These results suggest that 0.01, 0.1, 1 μmol/L puerarin promotes the proliferation of osteoblasts in older patients with osteoporosis in a concentration-dependent manner.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

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