中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (8): 1225-1230.doi: 10.3969/j.issn.2095-4344.1871

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

MiR-21可调控牙周炎破骨细胞的增殖

许  诺1,曹  蓁1,李晓杰2,史  春2   

  1. 1大连医科大学中山学院,辽宁省大连市  116000;2大连医科大学口腔医学院,辽宁省大连市  116000
  • 收稿日期:2019-05-17 修回日期:2019-05-25 接受日期:2019-06-29 出版日期:2020-03-18 发布日期:2020-01-22
  • 通讯作者: 史春,硕士,讲师,主治医师,大连医科大学口腔医学院,辽宁省大连市 116000
  • 作者简介:许诺,女,1984年生,辽宁省大连市人,硕士,讲师,主治医师,主要从事口腔医学相关教学与研究工作。 共同第一作者:曹蓁,女,1985年生,辽宁省大连市人,实验师,主要从事基础医学教学工作。
  • 基金资助:
    辽宁省自然科学基金(20180550563)

MicroRNA-21 regulates proliferation and differentiation of osteoclasts in periodontitis

Xu Nuo1, Cao Zhen1, Li Xiaojie2, Shi Chun2   

  1. 1Zhongshan College of Dalian Medical University, Dalian 116000, Liaoning Province, China; 2School of Stomatology, Dalian Medical University, Dalian 116000, Liaoning Province, China
  • Received:2019-05-17 Revised:2019-05-25 Accepted:2019-06-29 Online:2020-03-18 Published:2020-01-22
  • Contact: Shi Chun, Master, Lecturer, Attending physician, School of Stomatology, Dalian Medical University, Dalian 116000, Liaoning Province, China
  • About author:Xu Nuo, Master, Lecturer, Attending physician, Zhongshan College of Dalian Medical University, Dalian 116000, Liaoning Province, China Cao Zhen, Experimentalist, Zhongshan College of Dalian Medical University, Dalian 116000, Liaoning Province, China Xu Nuo and Cao Zhen contributed equally to this work.
  • Supported by:
    the Natural Science Foundation of Liaoning Province, No. 20180550563

摘要:

文题释义:

微小RNA:是由内源性基因编码的核苷酸非编码RNA分子,其表达具有组织特异性、保守性和时序性的特点。

牙周炎:是一种多因素感染性和免疫性炎症性疾病。它是定植微生物与宿主免疫系统之间复杂相互作用所导致的慢性感染性疾病,其特征是不可逆的组织病理学变化,如牙周韧带破坏、骨质破坏和牙周袋加深,这些都可以导致牙齿脱落。

背景:miR-21是破骨细胞生成的调节剂和破骨细胞分化的启动子,但是其在牙周炎中的作用尚不清楚。

目的:探讨miR-21在牙周炎骨破坏中的作用。

方法:Real-time PCR法检测并分析牙周炎样本和正常牙周膜组织中miR-21的表达差异;利用脂质体转染法,以miR-21 mimics(上调miR-21)或miR-21 inhibitor(下调miR-21)转染破骨细胞,Real-time PCR法检测miR-21及骨破坏标志因子TRAP与CTSK的表达变化;CCK-8检测miR-21对破骨细胞的增殖作用。

结果与结论:①miR-21在牙周炎样本中表达增高;②miR-21 mimics转染到破骨细胞后,miR-21、TRAP与CTSK mRNA的表达升高;miR-21 inhibitor转染到破骨细胞后,miR-21、TRAP与CTSK mRNA的表达下降;③miR-21 mimics转染到破骨细胞后,促进破骨细胞增殖;miR-21 inhibitor转染到破骨细胞后,抑制破骨细胞增殖;④结果表明,miR-21可以促进破骨细胞的增殖,结合miR-21可以促进骨破坏标志因子的表达,说明miR-21可以作为治疗牙周炎骨破坏的重要靶点。

ORCID: 0000-0002-4258-2470(许诺)

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

关键词:

牙周炎, RAW264.7细胞, 破骨细胞, miR-21, 骨破坏标志因子, 辽宁省自然科学基金

Abstract:

BACKGROUND: MicroRNA-21 (miR-21) is a regulator of osteoclastogenesis and a promoter of osteoclast differentiation, but its role in periodontitis remains unclear.

OBJECTIVE: To investigate whether miR-21 is involved in bone destruction in periodontitis.

METHODS: Real-time PCR was used to detect and analyze the differential expression of miR-21 in periodontitis samples. Using liposome transfection method, miR-21 mimics (up-regulating miR-21) or miR-21 inhibitor (down-regulating miR-21) was used to transfect osteoclasts. Expressions of miR-21 and bone destruction markers TRAP and CTSK were detected by real-time PCR. Cell counting kit-8 was used to detect the miR-21 effect on osteoclast proliferation.

RESULTS AND CONCLUSION: (1) MiR-21 expression increased in periodontitis samples. (2) When miR-21 mimics was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression increased; when miR-21 inhibitor was transfected into osteoclasts, miR-21, TRAP and CTSK mRNA expression decreased. (3) Transfection with miR-21 mimics promoted the proliferation of osteoclasts, while transfection with miR-21 inhibitor inhibited the proliferation of osteoclasts. To conclude, miR-21 can be used as an important target for the treatment of periodontitis.

Key words: Periodontitis, RAW264.7 cells, osteoclasts, miR-21, bone destruction marker, Natural Science Foundation of Liaoning Province

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