中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (34): 5501-5509.doi: 10.3969/j.issn.2095-4344.0686

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

采用静电纺丝技术制备丝素纤维蛋白/聚已内酯临时肩袖补片

郭岗岗1，2，庞亚博3，杨建华1，刘士臣1，高 爽4，肖统光5，张伟祥6，翟饶生1，郭全义2   

  1. 1佳木斯大学，黑龙江省佳木斯市 154007；2解放军总医院骨科研究所，再生医学北京市重点实验室，全军骨科战创伤重点实验室，北京市 100853；3徐州医科大学，江苏省徐州市 221004；4北京大学，北京市 100871；5亳州市人民医院，安微省亳州市 236800；6兰溪市人民医院，浙江省兰溪市 321100
  • 收稿日期:2018-07-24 出版日期:2018-12-08 发布日期:2018-12-08
  • 通讯作者: 郭全义，教授，解放军总医院骨科研究所，北京市 100853
  • 基金资助:

    国家自然科学基金(81472092，81772319)；国家重点研发计划课题(2017YFC1104102，2017YFC1103404)；北京市自然科学基金(7172203)；北京市科技专项(Z161100005016059)；佳木斯大学科学技术重点项目(12Z1201507)

Preparation of silk fibroin/polycaprolactone temporary rotator cuff patch by electrospinning technique

Guo Ganggang1, 2, Pang Yabo3, Yang Jianhua1, Liu Shichen1, Gao Shuang4, Xiao Tongguang5, Zhang Weixiang6, Zhai Raosheng1,   Guo Quanyi2   

  1. 1Jiamusi University, Jiamusi 154007, Heilongjiang Province, China; 2Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, PLA Key Laboratory of Orthopaedic Trauma, Beijing 100853, China; 3Xuzhou Medical University, Xuzhou 221004, Jiangsu Province, China; 4Peking University, Beijing 100871, China; 5Haozhou People’s Hospital, Haozhou 236800, Anhui Province, China; 6Lanxi People’s Hospital, Lanxi 321100, Zhejiang Province, China
  • Received:2018-07-24 Online:2018-12-08 Published:2018-12-08
  • Contact: Guo Quanyi, Professor, Institute of Orthopedics, Chinese PLA General Hospital, Beijing Key Laboratory of Regenerative Medicine, PLA Key Laboratory of Orthopaedic Trauma, Beijing 100853, China
  • Supported by:

    the National Nature Science Foundation of China, No. 81472092, 81772319; the National Key Research and Development Plan of China, No. 2017YFC1104102, 2017YFC1103404; Beijing Natural Science Foundation, No. 7172203; Beijing Science and Technology Special Project, No. Z161100005016059; the Key Science and Technology Project of Jiamusi University, No. 12Z1201507

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摘要：

文章快速阅读：

 

文题释义：
丝素纤维蛋白：是从蚕丝中分离、提取出来的一种天然蛋白质，含有18中氨基酸，具有良好的生物相容性，其表面可被化学修饰或者搭载生长因子，可被制成不同形状的材料，如薄膜、纤维、复合材料、管、微球等。
静电纺丝技术：是目前一种可以快速、规模化生产出直径为纳米级别的技术，利用高电场和接收轮的牵引将溶液的液滴在针头处由圆形变成圆锥形并在锥尖喷射、牵引的作用下在接收轮表面形成细丝。通过这种技术制备的静电纺丝膜孔隙率高、孔径小、比表面积大、成品纤维均一性好。


背景：目前肩袖撕裂术后二次断裂率比较高，肩袖补片被设计用于增强损伤肩袖的愈合，因此制备生物相容性好、可降解、生物力学性能好的临时肩袖补片为关键环节。
目的：通过静电纺丝技术制备出不同质量比例的丝素纤维蛋白/聚已内酯临时肩袖补片，评估其理化性质和生物相容性。
方法：采用静电纺丝技术制备丝素纤维蛋白与聚已内酯质量比分别为4∶0、3∶1、1∶1、1∶3、0∶4的临时肩袖补片，对其微观结构、接触角、降解性能及成分进行表征。将质量比3∶1、1∶1、1∶3、0∶4的临时肩袖补片分别植入SD大鼠背部皮下，植入后2，4周后取补片材料，进行苏木精-伊红染色。
结果与结论：①混合材料制备的临时肩袖补片，改善了单纯聚已内酯补片的直径、孔径数目、孔径直径及单纯丝素纤维蛋白补片脆性高而致的表面串珠样液滴；②混合材料制备的临时肩袖补片呈亲水特性，改善了单纯丝素纤维蛋白补片的高度亲水性及单纯聚已内酯补片的疏水性；③在混合材料制备的临时肩袖补片中，两种材料未发生化学反应；④除质量比0∶4的临时肩袖补片外，其余质量比临时肩袖补片浸泡于PBS中4周的质量均增加；⑤皮下植入后2周，各组有大量细胞密集黏附在肩袖补片周围，新生组织中有毛细血管形成，肩袖补片外面中间层拥有类似真正肌腱组织的微结构，纯聚已内酯补片外新生组织中层类似正常肌腱组织功能最差；⑥皮下植入后4周，贴附补片新生组织中的细胞数量明显减少，类似正常肌腱组织的外新生组织结构厚度增加，纯聚已内酯补片外新生组织中层类似正常肌腱组织功能最差；⑦结果表明，丝素纤维蛋白改善了聚已内酯补片诱导修复细胞向肌腱细胞分化的潜能。

ORCID: 0000-0002-8732-9612(郭岗岗)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 肩袖撕裂, 丝素纤维蛋白, 聚已内酯, 静电纺丝, 二次撕裂, 临时肩袖补片, 生物相容性, 接触角, 红外光谱, 扫描电镜, 生物材料

Abstract:

BACKGROUND: As the rate of re-fracture of the rotator is very high, the rotator cuff patch is designed to enhance the healing of the injured rotator cuff. Therefore, the preparation of temporary rotator cuff patch with good biocompatibility, biodegradability and biomechanical properties has become a committed step.

OBJECTIVE: To prepare a temporary rotator cuff patch with different ratio of silk fibroin (SF)/polycaprolactone (PCL) using electrospinning technique, and to evaluate its physiochemical properties and biocompatibility.
METHODS: Temporary rotator cuff patches with SF/PCL at different mass ratios of 4:0, 3:1, 1:1, 1:3, 0:4 were prepared using the electrospinning technique. Microstructure, contact angle, degradation properties and composition of the patches were characterized. Temporary rotator cuff patches with the mass ratios of 3:1, 1:1, 1:3, and 0:4 were implanted subcutaneously into the back of Sprague-Dawely rats. After 2 and 4 weeks of implantation, the patch material was taken for hematoxylin-eosin staining.
RESULTS AND CONCLUSION: (1) The temporary rotator cuff patches prepared from the mixed material improved the diameter, aperture number, and pore diameter of the pure PCL patch as well as the surface droplets caused by the high brittleness of the pure SF patch. (2) The temporary rotator cuff patches prepared by mixed materials improved hydrophilicity as compared with the SF patch and improved hydrophobicity as compared with the simple PCL patch. (3) In the temporary rotator cuff patch prepared by mixed materials, no chemical reaction occurred between the two materials. (4) With the exception of the temporary rotator cuff patch with a mass ratio of 0:4, the rest patches showed an increase in quality after immersed in PBS for 4 weeks. (5) Two weeks after subcutaneous implantation, a large number of cells were attached to the rotator cuff patch in each group, and capillaries formed in the neonatal tissues. The outer middle layer of the rotator cuff patch had a microstructure similar to the normal tendon tissue, while the middle layer of the pure PCL patch was the worst in microstructure. (6) Four weeks after subcutaneous implantation, the number of cells in the neonatal tissue of the patch significantly decreased, and the thickness of the outer neonatal tissue similar to the normal tendon tissue increased, while the pure PCL patch showed the worst function in the middle layer of the new tissue. To conclude, the results show that SF improves the potential of PCL patch to induce the cell differentiation into tendon cells.  

Key words: Fibrin, Materials Testing, Tissue Engineering

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