中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (30): 4888-4893.doi: 10.3969/j.issn.2095-4344.0990

• 生物材料综述 biomaterial review • 上一篇    下一篇

星孢菌素干预组织工程化颞下颌关节盘自组装基体收缩的机制

罗银月1,2,李鸿燕1,包广洁1,2,3,康 宏1   

  1. 1兰州大学口腔医学研究所,甘肃省兰州市 730000;2西北民族大学口腔医学国家民委重点实验室,甘肃省兰州市 730030;3西北民族大学甘肃省口腔疾病研究重点实验室,甘肃省兰州市 730030
  • 收稿日期:2018-05-26 出版日期:2018-10-28 发布日期:2018-10-28
  • 通讯作者: 包广洁,教授,硕士生导师,西北民族大学,甘肃省兰州市 730030
  • 作者简介:罗银月,女,1987年生,河南省信阳市人,汉族,硕士,主要从事颞下颌关节盘组织工程研究。
  • 基金资助:

    国家自然科学基金(81660189);西北民族大学中央高校基本科研业务费(2yp2015014)

Mechanisms of staurosporine intervention in tissue-engineered self-assembly construct contraction of the temporomandibular joint disc

Luo Yin-yue1, 2, Li Hong-yan1, Bao Guang-jie1, 2, 3, Kang Hong1   

  1. 1Institute of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; 2Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China; 3Key Laboratory of Oral Diseases of Gansu Province, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China
  • Received:2018-05-26 Online:2018-10-28 Published:2018-10-28
  • Contact: Bao Guang-jie, Professor, Master’s supervisor, Institute of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China; Key Laboratory of Oral Diseases of Gansu Province, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China
  • About author:Luo Yin-yue, Master candidate, Institute of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; Key Lab of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, Gansu Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81660189; the Fundamental Research Funds for the Central Universities, No. 2yp2015014

摘要:

文章快速阅读:

 

文题释义:
自组装技术:是组织工程中一个独特的方法,细胞在不需要支架依附的条件下,彼此之间利用细胞间黏附力最大化及总自由能最小化而聚集并结合在一起,形成功能上仿生的新生组织,细胞接种密度和不同的黏附力是组织性能的决定因素。
颞下颌关节关节盘:并不是由单一种类的细胞构成,不同物种关节盘细胞类型相同,但各细胞数量比例却不尽相同,根据细胞形态学分类普遍认为颞下颌关节关节盘主要由纤维样细胞和软骨样细胞构成,其中纤维样细胞占大多数。
 
 
背景:颞下颌关节盘细胞自组装基体在生长过程中出现的体积收缩现象机制不明,有研究认为可能与细胞骨架蛋白肌动蛋白的收缩效应有关。
目的:探索星孢菌素对颞下颌关节盘细胞、肌动蛋白和细胞外基质代谢的影响,阐明可能导致自组装基体收缩的机制。
方法:由第一作者检索2000至2018年PubMed、Web of Science核心合集数据库相关文献,检索关键词为“staurosporine,temporomandibular joint disc tissue engineering,chondrocyte tissue engineering,self-assembly”;检索2000至2018年CNKI中文数据库相关文献,中文检索词为“星孢菌素,颞下颌关节盘组织工程,软骨细胞组织工程,自组装”。

结果与结论:颞下颌关节盘细胞自组装基体收缩的可能原因:体外关节盘细胞的收缩;细胞类型的不同;基体营养通路障碍;肌动蛋白过表达;细胞外基质减少。星孢菌素减少肌动蛋白细胞骨架的表达,抑制细胞收缩,上调细胞外基质中重要基质蛋白胶原、聚集蛋白聚糖及软骨寡聚基质蛋白的量,抑制基体收缩。

ORCID: 0000-0003-0921-6548(罗银月)

关键词: 星孢菌素, 颞下颌关节盘组织工程, 软骨细胞组织工程, 自组装, 生物材料

Abstract:

BACKGROUND: The mechanism of construct contraction in the process of self-assembly of the temporomandibular joint (TMJ) disc cells is still unknown. Some studies have suggested that it may be related to the contractile effect of cytoskeleton actin.

OBJECTIVE: To investigate the effects of staurosporine on actin and extracellular matrix (ECM) metabolism of TMJ disc cells in vitro and to elucidate the possible mechanism underlying the self-assembly construct contraction.
METHODS: PubMed, Web of Science and CNKI database (2000-2018) were retrieved by the first author with the key words of “staurosporine, temporomandibular joint disc tissue engineering, chondrocyte tissue engineering, self-assembly” in English and Chinese, respectively, to search relevant articles about the use of staurosporine in the tissue engineering of temporomandibular joint disc.

RESULTS AND CONCLUSION: Possible contraction causes of TMJ cell self-assembly construct include: contraction of articular disc cells in vitro, differences in cell types, nutritional disorders of the construct, overexpression of actin, and reduced extracellular matrix. Staurosporine reduces the expression of actin cytoskeleton, inhibits cell contraction and up-regulates the amount of important matrix proteins, collagen, aggrecan, and cartilage oligomeric matrix proteins in the extracellular matrix, thereby suppressing the contraction of the self-assembly construct.

Key words: Extracellular Matrix, Temporomandibular Joint Disk, Actins, Tissue Engineering

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