中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (9): 1319-1323.doi: 10.3969/j.issn.2095-4344.2017.09.002

• 肿瘤干细胞 cancer stem cells • 上一篇    下一篇

干细胞表面标志CD133在肝癌中的表达及其阳性亚群增殖特性

张宝芹   

  1. 北京大学滨海医院、天津市第五中心医院消化科,天津市  300450
  • 出版日期:2017-03-28 发布日期:2017-03-31
  • 作者简介:张宝芹,女,1975年生,天津市人,汉族,硕士,主治医师,主要从事肝癌治疗以及肝脏病研究。
  • 基金资助:

    国家青年科学基金项目(81770221):膜联蛋白A2亚硝基化诱导肝细胞癌上皮-间质转化的机制

Expression of stem cell surface marker CD133 in hepatocellular carcinoma and its positive subset proliferation

Zhang Bao-qin   

  1. Department of Digestion, Binhai Hospital of Peking University, Fifth Central Hospital of Tianjin, Tianjin 300450, China
  • Online:2017-03-28 Published:2017-03-31
  • About author:Zhang Bao-qin, Master, Attending physician, Department of Digestion, Binhai Hospital of Peking University, Fifth Central Hospital of Tianjin, Tianjin 300450, China
  • Supported by:

    the National Youth Science Foundation of China, No. 81770221

摘要:

文章快速阅读:


文题释义:
CD133:
是一种重要的表面标志物,具有独特的信号传递通路,可参与到淋巴细胞再循环过程中,与肿瘤细胞之间存在十分密切的联系。以CD133为标志物,利用其蛋白特异性特征可对不同肿瘤干细胞进行分选和研究。此次实验中,即以CD133为标志物对人肝癌MHCC97-H细胞株进行培养和分选,获得CD133+/CD133- MHCC97-H细胞。
Notch信号途径:是一种细胞间相互作用的基本途径,调控着细胞增殖、分化、凋亡等过程。在Notch途径被激活时,干细胞发生增殖,活性受到抑制时,干细胞开始发生分化。

 

摘要
背景:
以CD133为标志物,利用其蛋白特异性特征可对不同的肿瘤干细胞进行分选和研究。
目的:探讨干细胞表面标志CD133在肝癌中的表达及其阳性亚群的体外增殖特性。
方法:对人肝癌MHCC97-H细胞株进行培养和分选,分别获得CD133+与CD133-MHCC97-H细胞,检测CD133表达及细胞迁移侵袭能力;培养14 d,检测细胞克隆形成率;培养7 d,检测细胞增殖及Notch基因蛋白表达。将CD133+与CD133-MHCC97-H细胞分别接种于裸鼠背部皮下,4周后,检测成瘤情况。
结果与结论:①CD133表达与细胞克隆形成率:CD133+MHCC97-H细胞CD133表达率、克隆形成率均显著大于CD133-MHCC97-H细胞(P < 0.05);②细胞增殖:CD133+MHCC97-H细胞培养3-7 d的吸光度值大于CD133-MHCC97-H细胞(P < 0.05);③细胞迁移侵袭:CD133+MHCC97-H细胞迁移与侵袭实验的透膜细胞数均显著多于CD133-MHCC97-H细胞(P < 0.05);④Notch基因蛋白:CD133+MHCC97-H细胞Notch基因蛋白表达多于CD133-MHCC97-H细胞(P < 0.05);⑤成瘤实验:CD133-MHCC97-H细胞组成瘤体积大于CD133-MHCC97-H细胞组(P < 0.05);⑥结果表明:CD133阳性肝癌细胞亚群具有较强的体外增殖特性,具有一定的肿瘤干细胞特性,并有较强的侵袭、转移及致瘤能力。

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0002-5015-9381(张宝芹)

关键词: 干细胞, 肿瘤干细胞, 肝癌, 干细胞表面标志, 细胞亚群, 细胞增殖, 侵袭, 转移, 干细胞特性, 移植瘤, 国家自然科学基金

Abstract:

 

BACKGROUND: CD133 has been used as a marker to separate and study different tumor stem cells by using its protein specific features.
OBJECTIVE: To investigate the expression of stem cell surface marker CD133 in hepatocellular carcinoma and the in vitro proliferation of its positive subsets.
METHODS: The human hepatoma MHCC97-H cell lines were cultured and sorted, CD133+ and CD133- MHCC97-H cells were obtained, and the expression of CD133 and cell migration and invasion were detected. After 7 days of culture, cell proliferation ability and Notch expression were detected. After 14 days of culture, cell cloning efficiency was detected. CD133+ and CD133- MHCC97-H cells were implanted into nude mice subcutaneously, and 4 weeks later, tumor formation in mice was detected.

RESULTS AND CONCLUSION:(1) CD133 expression and cell clone formation: The expression of CD133 and cell cloning efficiency of CD133+ MHCC97-H cells were significantly higher than those of CD133- MHCC97-H cells (P < 0.05). (2) Cell proliferation: After 3-7 days of culture, the absorbance value of CD133+ MHCC97-H cells was significantly higher than that of CD133- MHCC97-H cells (P < 0.05). (3) Cell migration and invasion: The number of CD133+ MHCC97-H cells passing through the cell membrane was significantly more than that of CD133- MHCC97-H cells (P < 0.05). (4) The Notch protein expression of CD133+ MHCC97-H cells was significantly higher than that of CD133- MHCC97-H cells (P < 0.05). (6) Tumor formation test: The tumor size of CD133 - MHCC97-H cells was larger than that of CD133- MHCC97-H cells (P < 0.05). To conclude, CD133+ hepatocellular carcinoma cell subsets with strong proliferation ability have some characteristics of cancer stem cells, and strong invasion, metastasis and tumorigenic abilities.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Neoplastic Stem Cells, Cell Proliferation, Tissue Engineering

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