中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (49): 7964-7968.doi: 10.3969/j.issn.2095-4344.2015.49.016

• 脑及脊髓损伤动物模型 Animal models of brain and spinal cord injuries • 上一篇    下一篇

X染色体上脆性X智力低下基因1敲除模型小鼠针刺长强穴学习记忆及海马CA1区γ-氨基丁酸A型受体α1亚基表达的影响

吴 强1，陈丽云2，张学君1   

  1. 1福建中医药大学针灸学院，福建省福州市 350122；2福州市中医院，福建省福州市  350001
  • 收稿日期:2015-09-18 出版日期:2015-11-30 发布日期:2015-11-30
  • 作者简介:吴强，2003年广州中医药大学毕业，博士，教授，博士生导师，主要从事儿童脑病(脑瘫、智障、自闭)基础与临床方面的研究。
  • 基金资助:

    国家自然科学基金项目(81373720)；福建省科技厅社发处重点项目(2012Y0045)

Effects of acupuncturing Changqiang acupoint on the learning and memory function and gamma-aminobutyric acid type A receptor alpha1 subunit expression in the hippocampal CA1 region of the fragile X mental retardation 1 located on X chromosome knockout mice 

Wu Qiang1, Chen Li-yun2, Zhang Xue-jun1   

  1. 1College of Acupuncture and Massage, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China; 2Chinese Medicine Hospital of Fuzhou, Fuzhou 350001, Fujian Province, China
  • Received:2015-09-18 Online:2015-11-30 Published:2015-11-30
  • About author:Wu Qiang, M.D., Professor, Doctoral supervisor, College of Acupuncture and Massage, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81373720; the Key Project of Social Development Division of Fujian Provincial Science and Technology Department, No. 2012Y0045

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摘要：

背景：X染色体上脆性X智力低下基因1敲除小鼠海马区γ-氨基丁酸A型受体α1亚基表达低于野生型小鼠。
目的：观察针刺长强穴对X染色体上脆性X智力低下基因1敲除小鼠学习记忆及海马CA1区γ-氨基丁酸A型受体α1亚基表达的影响。
方法：采用X染色体上脆性X智力低下基因1敲除纯合子小鼠雌雄各5只，一雌一雄合笼饲养。所繁殖幼鼠经基因型鉴定为X染色体上脆性X智力低下基因1敲除纯合子小鼠者，随机分为模型组、长强组、非穴组，各10只，另取野生型小鼠10只作为空白组。长强组小鼠采用平补平泻，行提插手法针刺长强穴1 min，频率160-200次/min，1次/d，连续治疗10 d；非穴组刺激小鼠右肋弓最低点上1 cm处；模型组及空白组每日只进行模拟抓取。
结果与结论：与空白组相比，模型组小鼠水迷宫逃避潜伏期明显延长(P < 0.05)；而与模型组相比，长强组小鼠水迷宫逃避潜伏期明显缩短(P < 0.05)，海马CA1区γ-氨基丁酸A型受体α1亚基表达率增加(P < 0.05)；而非穴组小鼠水迷宫逃避潜伏期及海马CA1区γ-氨基丁酸A型受体α1亚基表达率与模型组接近(P > 0.05)。且针刺X染色体上脆性X智力低下基因1敲除小鼠长强穴后其海马CA1区γ-氨基丁酸A型受体α1亚基阳性细胞率与逃避潜伏期呈负相关(r=-0.554，P=0.001)。提示针刺长强穴可改善X染色体上脆性X智力低下基因1敲除小鼠的学习记忆能力，上调γ-氨基丁酸A型受体α1亚基在海马CA1区的表达。 

 

关键词: 实验动物模型, 脑及脊髓损伤动物模型, 长强穴, 脆性X综合征, 针刺, 学习记忆能力, γ-氨基丁酸A型受体α1亚基, 国家自然科学基金

Abstract:

BACKGROUND: γ-Aminobutyric acid type A receptor α1 subunit expression was lower in the hippocampus of mice of fragile X mental retardation 1 (FMR1) gene located on the X chromosome knockout than in wild-type mice.
OBJECTIVE: To study the effect of acupuncturing Changqiang (DU1) point on the learning and memory function and γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region of the FMR1 located on X chromosome knockout mice.
 
METHODS: There were five male and five female homozygous mice of FMR1 located on X chromosome knockout. One female and one male were housed in a cage. Their offspring that was identified as homozygous mice of FMR1 located on X chromosome knockout were randomly divided into model group, Changqiang group, and non-acupuncture group (n=10). An additional 10 wild-type mice were considered as blank group. Mice in the Changqiang group received acupuncture at Changqiang acupoint for 1 minute by mild reinforcing and attenuating method, and lifting and inserting method, at the frequency of 160-200 times/minute, once a day, for 10 consecutive days. Mice in the non-acupuncture group were acupunctured at 1 cm above the lowest point of mouse right costal arch. Mice in the model and blank groups only received analog crawl.
RESULTS AND CONCLUSION: Compared with the blank group, the escape latency in the water maze was significantly prolonged in the model group (P < 0.05). Compared with the model group, the escape latency in the water maze was significantly shorter in the Changqiang group (P < 0.05), and the γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region was increased (P < 0.05). The escape latency in the water maze and γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region were similar between the non-acupuncture and model groups (P > 0.05). Moreover, γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region was negatively associated with the escape latency after acupuncturing the Changqiang acupoint (r=-0.554, P=0.001). Data suggested that acupuncture at Changqiang acupoint improved the learning and memory capabilities of mice with the FMR1 located on X chromosome knockout, and up-regulated γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region.  

 

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