中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5891-5896.doi: 10.3969/j.issn.2095-4344.2012.32.001

• 脐带脐血干细胞 umbilical cord blood stem cells •    下一篇

直接和间接共培养条件下人脐带间充质干细胞对恶性胶质瘤细胞系 SHG44生长的抑制作用

梁阿铭1，2，梁 硕3，樊锐太4，田 毅1，2，迟连凯3，史辛艺3，刘金瑜1，2，关方霞3，杨 波1，2   

  1. 郑州大学第一附属医院1神经外科，
    4放疗科，河南省郑州市450052；
    2河南省高等学校临床医学重点学科开放实验室，河南省郑州市 450052；
    3郑州大学生物工程系，河南省郑州市 450001
  • 收稿日期:2012-05-02 修回日期:2012-06-28 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 杨波，硕士，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市 450052 yangbo96@126.com 并列通讯作者：关方霞，博士，教授，郑州大学生物工程系，河南省郑州市 450001 guanfangxia@126.com
  • 作者简介:梁阿铭★，男，1987年生，河南省博爱县人，汉族，郑州大学医学院在读硕士，主要从事间充质干细胞和神经干细胞方面的研究。 liangaming1986@126.com

Human umbilical cord mesenchymal stromal cells inhibit the growth of SHG44 cells under direct and indirect co-cultivation

Liang A-ming1, 2, Liang Shuo3, Fan Rui-tai4, Tian Yi1, 2, Chi Lian-kai3, Shi Xin-yi3, Liu Jin-yu1, 2, Guan Fang-xia3, Yang Bo1, 2   

  1. 1Department of Neurosurgery, 4Department of Radiotherapy, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China;
    2Open Laboratory of Clinical Medical Key Subjects, Henan Higher Education Institute, Zhengzhou 450052, Henan Province, China;
    3Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China
  • Received:2012-05-02 Revised:2012-06-28 Online:2012-08-05 Published:2012-08-05
  • Contact: Yang Bo, Master, Professor, Doctoral supervisor, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Clinical Medical Key Subjects, Henan Higher Education Institute, Zhengzhou 450052, Henan Province, China yangbo96@126.com Co-corresponding author: Guan Fang-xia, M.D., Professor, Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China guanfangxia@126.com
  • About author:Liang A-ming★, Studying for master's degree, Department of Neurosurgery, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Open Laboratory of Clinical Medical Key Subjects, Henan Higher Education Institute, Zhengzhou 450052, Henan Province, China liangaming1986@126.com

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375

被引次数

2

摘要：

背景：SHG44是一种恶性胶质瘤细胞系，其生物学性状稳定、易于培养，广泛应用于动物成瘤模型制作，目前对其研究主要集中在运用药物、基因转染及电离辐射等途径抑制其生长，未见间充质干细胞对其作用的相关报道。
目的：通过直接和间接共培养的方法，探讨人脐带间充质干细胞对恶性胶质瘤细胞系SHG44的作用，观察SHG44胶质瘤细胞数量及细胞周期的变化。
方法：将人脐带间充质干细胞与SHG44细胞，分别在24孔板进行直接共培养，在Transwell板进行间接共培养，分别设对照组。培养3 d后在荧光显微镜下观察，并收集Transwell板中的SHG44胶质瘤细胞，应用流式细胞术分别检测SHG44细胞周期。
结果与结论：直接接触共培养组SHG44胶质瘤细胞数量少于对照组(P < 0.05)。Transwell间接接触共培养组SHG44胶质瘤细胞数量与对照组相比，差异无显著性意义(P > 0.05)，其细胞周期位于G0/G1期的SHG44胶质瘤细胞比率高于对照组(P < 0.05)。证实，体外培养的人脐带间充质干细胞可以通过直接及间接接触抑制恶性胶质瘤细胞系SHG44细胞的生长。

关键词: 直接共培养, 间接共培养, 脐带间充质干细胞, SHG44细胞, 抑制作用, 恶性胶质瘤细胞, 干细胞

Abstract:

BACKGROUND: SHG44, a kind of malignant glioma cell line, is easy to cultivate with stable biomedical properties and widely used in the animal models of tumors. The current studies focus on inhibiting SHG44 growth by drugs, gene transfection and ionizing radiation. There have been no reports describing the effects of mesenchymal stem cells on biological characteristics of SHG44.
OBJECTIVE: To investigate the cell cycle and the number of SHG44 cells which were directly and indirectly co-cultured with human umbilical cord mesenchymal stem cells.
METHODS: Human umbilical cord mesenchymal stem cells and SHG44 cells were directly co-cultured in the 24-well plate and indirectly co-cultured in the Transwell plate. A control group was designed for the direct and indirect co-culture separately. After co-culture for 3 d, cells were observed under fluorescence microscope, and SHG44 cells were collected for detection of SHG44 cell cycle by flow cytometry.
RESULTS AND CONCLUSION: Compared with corresponding controls, the number of SHG44 cells fluorescently labeled decreased after directly co-cultured with MSCs (P < 0.05); the number of SHG44 cells fluorescently labeled had no change after indirectly co-cultured with MSCs (P > 0.05). The proportion of SHG44 cells at G0/G1 stage was significantly higher than that in the control group (P < 0.05). The results confirmed that human umbilical cord mesenchymal stem cells cultured in vitro can inhibit the proliferation of SHG44 cells by direct and indirect ways in vitro.

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