中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (31): 5777-5782.doi: 10.3969/j.issn.2095-4344.2012.31.018

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

环孢素A对小鼠气管移植模型中气管上皮细胞的影响

李 超，鲍 方，王林毛，郑 卉，陈 昶，高 文   

  1. 上海市肺科医院胸外科，上海市200433
  • 收稿日期:2012-01-14 修回日期:2012-02-29 出版日期:2012-07-29 发布日期:2012-07-29
  • 通讯作者: 陈昶，主任医师 上海市肺科医院胸外科，上海市 200433 changchenc@hotmail.com
  • 作者简介:李超★，男，1987年生，江西省抚州市人，汉族，同济大学在读硕士，主要从事胸外科气管移植研究。 lichaocelly@163.com 并列第一作者：鲍方★，男，1985年生，安徽省安庆市人，汉族，苏州大学在读硕士，主要从事胸外科气管移植研究。

Effects of cyclosporine A on epithelial cells in a mouse trachea transplantation model

Li Chao, Bao Fang, Wang Lin-mao, Zheng Hui, Chen Chang, Gao Wen   

  1. Department of General Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China
  • Received:2012-01-14 Revised:2012-02-29 Online:2012-07-29 Published:2012-07-29
  • Contact: Chen Chang, Chief physician, Department of General Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China changchenc@hotmail.com
  • About author:Li Chao★, Studying for master’s degree, Department of General Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China lichaocelly@163.com Bao Fang, Studying for master’s degree, Department of General Thoracic Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China Li Chao and Bao Fang contributed equally to this study.

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摘要：

背景：气管上皮的再生及完整性对预防肺移植后闭塞性细支气管炎起着最为关键的作用。
目的：观察环孢素A对小鼠气管异位移植模型中移植气管上皮细胞增殖的影响。
方法：将BALB/c小鼠气管异位移植到C57BL/6小鼠颈背部皮下，建立异位气管移植模型，将受体小鼠随机分组：实验组腹腔注射环孢素A 25 mg/(kg•d)，对照组不注射环孢素A。
结果与结论：移植气管病理检查显示，环孢素A具有延缓上皮细胞剥离、减少淋巴细胞浸润以及延缓纤维化过程的作用，但最终仍无法阻止移植物管腔闭塞的发生。上皮细胞定量分析显示，环孢素A可以延缓上皮纤毛细胞下降幅度，延缓上皮细胞增殖高峰期，加快上皮细胞凋亡高峰期。细胞因子检测显示，环孢素A对白细胞介素4分泌具有促进作用，对干扰素γ分泌具有早期促进，中晚期抑制作用。

关键词: 气管移植动物模型, 环孢素A , 上皮细胞, 闭塞性气道疾病, 小鼠

Abstract:

BACKGROUND: The regeneration and integrity of tracheal epithelial plays a key role in the prevention of bronchiolitis obliterans after lung transplantation.
OBJECTIVE: To study the effects of cyclosporine A administration on the proliferation of epithelial cells in heterotopic transplanted trachea models.
METHODS: Tracheas from BALB/c mice were heterotopically transplanted to C57BL/6 mice to establish the heterotopic transplanted trachea model. The recipient mice were randomly divided, recipient mice in the experimental group were received 25mg/(kg•d) cyclosporine A injection, while the rest recipients in the control group did not have cyclosporine A injection.
RESULTS AND CONCLUSION: The pathological examination of transplanted trachea showed that cyclosporine A could delay the progress of epithelial cells’ stripping and fibrosis and depress the lymphocyte infiltration in heterotopic tracheal transplantation model. However, cyclosporine A did not prevent the final occurrence of graft obliteration. Quantitative analysis showed that cyclosporine A could slow down the decreasing process of epithelial ciliated cell and the proliferation peaks of epithelial cells and accelerate the apoptosis peaks of epithelial cells. Cytokines measurement showed that cyclosporine A could promote the secretion of interleukin-4 and interferon gamma γ in the early days after transplantation, and it could inhibit the secretion in the middle and advanced stage.

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