中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (31): 5701-5705.doi: 10.3969/j.issn.2095-4344.2012.31.001

• 肝移植 liver transplantation •    下一篇

吲哚胺2，3-二氧化酶转染供体可抑制大鼠肝移植的急性排斥反应

刘晓珑1，韩鲁浙1，任 鑫1，肖 刚1，金一怡1，陈永兵2，刘立新1   

  1. 1南方医科大学第三附属医院普通外科，广东省广州市 510630；
    2解放军北京军区总医院普外科，北京市 100007
  • 收稿日期:2012-01-27 修回日期:2012-03-07 出版日期:2012-07-29 发布日期:2012-07-29
  • 通讯作者: 刘立新，博士，主任医师，南方医科大学第三附属医院普通外科，广东省广州市 510630 liulixin@tom.com
  • 作者简介:刘晓珑☆，男，1979年生，山西省太原市人，汉族，2008年四川大学毕业，博士，主治医师，主要从事肝胆外科的研究。 g_xlliu@126.com

Inhibition of acute allograft rejection in a rat liver transplantation model by adenovirus associated plasmid transferred with indoleamine 2,3-dioxygenase

Liu Xiao-long1, Han Lu-zhe1, Ren Xin1, Xiao Gang1, Jin Yi-yi1, Chen Yong-bing2, Liu Li-xin1   

  1. 1Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China;
    2Department of General Surgery, General Hospital of Beijing Military Command, Beijing 100007, China
  • Received:2012-01-27 Revised:2012-03-07 Online:2012-07-29 Published:2012-07-29
  • Contact: Liu Li-xin, Doctor, Chief physician, Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China
  • About author:Liu Xiao-long☆, Doctor, Attending physician, Department of General Surgery, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, Guangdong Province, China g_xlliu@126.com

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摘要：

背景：吲哚胺2，3-二氧化酶(indoleamine 2，3-dioxygenase，IDO)表达于免疫特赦器官中，能选择性诱导T细胞免疫耐受。
目的：观察腺病毒相关质粒载体介导IDO转染供体对大鼠肝移植急性排斥反应的抑制作用。
方法：构建携带IDO的重组腺病毒质粒pWCM-IDO，建立PVG/DA大鼠肝移植模型48对，随机分为3组，生理盐水组、pWAV2组、pWCM-IDO组分别腹腔注射生理盐水、pWCM悬液和pWCM-IDO悬液1 mL。
结果与结论：pWCM-IDO可成功转染PVG供体肝脏，PWCM-IDO组生存期和IDO表达明显高于生理盐水组和pWAV2组(P < 0.05~0.01)；移植后第4天PWCM-IDO组急性排反应程度明显弱于其他2组。提示IDO的重组腺病毒质粒可成功转染大鼠肝脏，缓解急性排斥反应，但难以持续有效表达，对免疫排斥的抑制作用有限。

关键词: 吲哚胺2, 3-二氧化酶, 肝移植, 急性排斥反应, 转染, 免疫抑制

Abstract:

BACKGROUND: Indoleamine 2,3-dioxygenase (IDO) expressing in immune privilege site can induces the T cell immune tolerance.
OBJECTIVE: To study the inhibitory effect of adenovirus associated plasmid vector medicated IDO on acute rejection following rat liver allograft.
METHODS: IDO and pWAV2 were transferred into a new vector pWCM-IDO. Forty-eight PVG/DA liver transplant models were randomly devided into three groups: normal saline group, pWAV2 group and pWCM-IDO group. Models in the three groups were performed with intraperitoneal injection of 1 mL normal saline, pWAV2 and pWCM-IDO, respectively.
RESULTS AND CONCLUSION: pWCM-IDO could be successfully transfected into the PVG donor liver. The survival time and the IDO expression level in the PWCM-IDO group were significantly higher than those in the normal saline group and pWAV2 group (P < 0.05-0.01). At 4 days after transplantation, the acute rejection degree in the PWCM-IDO group was significantly weaker than that in the other two groups. IDO recombinant plasmid could be successfully transfected into rat liver to relieve the acute rejection following liver allograft. But it difficult to sustain the effective expression, and the inhibitory effect on immune rejection was limited.

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