中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5154-5159.doi: 10.3969/j.issn.2095-4344.2012.28.006

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

联合运用维生素D3和动物油建立小鼠钙化性血管损伤模型

谢爱媚，司徒永立，吴 铁   

  1. 广东医学院药理教研室，广东省湛江市 524023
  • 收稿日期:2012-04-09 修回日期:2012-05-25 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 吴铁，硕士，教授，硕士生导师，广东医学院药理教研室，广东省湛江市 524023 wutie2@163.com
  • 作者简介:谢爱媚★，女，1986年生，广东省梅县人，汉族，广东医学院在读硕士，主要从事心血管药理和骨科药理研究工作。 xieaimei0325@126.com

Combination of vitamin D3 and animal oil induces calcified vascular injury in mice

Xie Ai-mei, Situ Yong-li, Wu Tie   

  1. Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • Received:2012-04-09 Revised:2012-05-25 Online:2012-07-08 Published:2012-07-08
  • Contact: Wu Tie, Master, Professor, Master’s supervisor, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China wutie2@163.com
  • About author:Xie Ai-mei★, Studying for master’s degree, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China xieaimei0325@126.com

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摘要：

背景：现有的小鼠血管损伤模型难以使小鼠主动脉同时出现钙化和损伤这两种病理变化。
目的：联合运用超生理剂量维生素D3和动物油建立小鼠钙化性血管损伤模型。
方法：连续4 d灌胃给予小鼠3×105 U/(kg • d)维生素D3，第5~42天灌胃给予5 mL/(kg • d)动物油，建立小鼠钙化性血管损伤模型。
结果与结论：模型小鼠血脂、血钙和血磷水平均无显著变化(P > 0.05)，ICP等离子发射光谱仪检测显示模型小鼠腹主动脉钙、磷含量升高(P < 0.05)，苏木精-伊红染色可见模型小鼠胸主动脉出现钙化斑块，斑块破裂造成血管壁缺损，新生内膜形成，血管弹性纤维延展性下降、断裂，中膜平滑肌细胞大量凋亡，局部增生形成纤维斑块等病理改变。说明联合运用超生理剂量维生素D3和动物油可以成功建立小鼠钙化性血管损伤模型。

关键词: 维生素D3, 动物油, 小鼠, 钙化, 血管损伤, 钙化性血管损伤模型

Abstract:

BACKGROUND: Vascular injury models of mice are hardly to have two pathological changes of calcification and injury, simultaneously and extensively, especially in the aorta with non-surgical treatments.
OBJECTIVE: To use large-dose vitamin D3 and animal oil to induce calcified vascular injury in mice.
METHODS: The model mice were fed with 3×105 U/ (kg • d) vitamin D3 for 4 days prior to 5 mL/(kg • d) animal oil feeding from day 5 to day 42.
RESULTS AND CONCLUSION: The contents of serum lipids, calcium and phosphorus in the model group mice had no significant changes (P > 0.05). Meanwhile, the calcium and phosphorus contents in the abdominal aorta were significantly increased in the model group (P < 0.05). Hematoxylin-eosin staining revealed the pathological changes of the thoracic aorta, such as obvious calcification plaques, which appeared on the vessel walls and might rupture and result in defects, then the neointimal forming. Besides, eastic fibers presented with lower ductility and breakage. Apoptosis was induced greatly in media vascular smooth muscle cells, and fibrous plaques were locally present due to the proliferation of vascular smooth muscle cells. The calcified vascular injury model in mice can be established by a combination of vitamin D3 and animal oil.

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