中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (18): 3263-3266.doi: 10.3969/j.issn.1673-8225.2012.18.008

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

激素对脑死亡猪心脏功能的保护及血清降钙素基因相关肽和内皮素1的影响**

王  曦，马利明，贾少军，杨绍军   

  1. 昆明医学院第二附属医院胸心血管外科，云南省昆明市　650101
  • 收稿日期:2011-12-24 修回日期:2012-01-24 出版日期:2012-04-29 发布日期:2012-04-29
  • 通讯作者: 杨绍军，主任医师，硕士生导师，昆明医学院第二附属医院胸心血管外科，云南省昆明市 650101 yangsj8@ hotmail.com
  • 作者简介:王曦，男，1973年生，安徽省宿州市人，1996年北京大学医学部毕业，主治医师，主要从事胸心血管外科工作。wangxi1a2b@ sina.com
  • 基金资助:

    云南省科技厅-昆明医学院联合专项课题(2008CD026)；云南省卫生科技计划项目基金(2009NS076)。

Effects of hormone on heart function protection and the levels of serum calcitonin-gene-related peptide and endothelin-1 in brain-dead pigs

Wang Xi, Ma Li-ming, Jia Shao-jun, Yang Shao-jun   

  1. Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming  650101, Yunnan Province, China
  • Received:2011-12-24 Revised:2012-01-24 Online:2012-04-29 Published:2012-04-29
  • Contact: Yang Shao-jun, Chief physician, Master’s supervisor, Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China yangsj8@hotmail. com
  • About author:Wang Xi, Attending physician, Department of Thoracic and Cadiovascular Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China wangxi1a2b@sina. com
  • Supported by:

    Joint Special Projects of Science and Technology Department of Yunnan Province-Kunming Medical College, No.2008CD026*; Health Science and Technology Projects Fund of Yunnan Province, No.2009NS076*

PDF

465

摘要：

背景：激素可以减轻脑死亡供体心脏的缺血再灌注损伤，减少炎症因子释放，起到保护脑死亡后供体心脏的作用。
目的：进一步验证激素对脑死亡猪心脏功能的保护作用及血清中降钙素基因相关肽和内皮素1的影响。
方法：版纳小耳猪14头，随机分为对照组和糖皮质激素组。用缓慢颅内加压法建立脑死亡模型，激素组予模型建立前半小时6 mg/kg甲泼尼龙琥珀酸钠静脉注入，对照组不用激素。分别予成模后0，1，3，6，9 h监测平均动脉压、左室射血分数，并测定血清中降钙素基因相关肽和内皮素1水平。
结果：激素组各时段平均动脉压、左室射血分数明显高于对照组(P < 0.05)。激素组血清中降钙素基因相关肽下降缓慢，内皮素1升高延缓，与对照组相比差异有显著性意义(P < 0.001)。提示激素可有效保护脑死亡期的心脏功能，其机制可能与减少炎症反应、增加心肌供能、维持内环镜稳定有关。
.18.008

关键词: 激素, 脑死亡, 降钙素基因相关肽, 内皮素1, 左室射血分数, 平均动脉压

Abstract:

BACKGROUND: Hormones can reduce the heart ischemia-reperfusion injury of brain-dead donor and reduce the release of inflammatory factors, and play a role in the protection of the donor heart after brain death. 
OBJECTIVE: To investigate the effects of hormone on heart function of brain-dead pigs and on the level of serum calcitonin-gene-related peptide (CGRP) and endothelin-1.
METHODS: Fourteen healthy Xishuangbanna Miniature pigs were randomly divided into hormonal group and control group. The brain death model was established by slow intracranial pressure method. The pigs in the hormonal group were performed with intravenous injection of 6 mg/kg methylprednisolone sodium succinate at 30 minutes before establishment, and the pigs in the control group were not treated with hormone. The mean arterial pressure, left ventricular ejection fraction and the CGRP and endothelin-1 level in serum were measured at 0, 1, 3, 6 and 9 hours after brain death.
RESULTS AND CONCLUSION: The mean arterial pressure and left ventricular ejection fraction in the hormonal group were significantly higher than those in the control group (P < 0.05). The level of CGRP was gradually decreased and the level of endothelin-1 was gradually increased in the hormonal group, there was significant difference between hormonal group and control group (P < 0.001). Hormone can protect heart function effectively in the brain dead pig donor, which might be related to the decreasing of inflammatory reaction, the increasing of energy support of heart and maintenance of internal environment stabilization.

中图分类号: