中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2487-2390.doi: 10.3969/j.issn.1673-8225.2012.14.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

大鼠骨髓间充质干细胞的PKH26标记和示踪☆

彭  艳，何援利，朱少芳   

  1. 南方医科大学珠江医院妇产科，广东省广州市 510282
  • 收稿日期:2011-08-26 修回日期:2011-09-30 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 何援利，主任医师，教授，南方医科大学珠江医院妇产科，广东省广州市 510282 heyuanli310@yahoo.com.cn
  • 作者简介:彭艳☆，女，1978年生，江苏省海门市人，汉族，南方医科大学在读博士，主要从事子宫内膜异位症及干细胞的研究。 pengyansisi@126.com

Rat bone marrow mesenchymal stem cells labeled and tracked with PKH26  

Peng Yan, He Yuan-li, Zhu Shao-fang   

  1. Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong  Province, China
  • Received:2011-08-26 Revised:2011-09-30 Online:2012-04-01 Published:2012-04-01
  • Contact: author: He Yuan-li, Chief physician, Professor, Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China yahoo.com.cn
  • About author:Peng Yan☆, Studying for doctorate, Department of Obstetrics and Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China pengyansisi@126.com

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摘要：

背景：骨髓间充质干细胞标记是体内迁移分化研究的重要环节。
目的：对大鼠骨髓间充质干细胞进行PKH26标记，探讨PKH26标记对骨髓间充质干细胞的生长特征、分化的影响及体内示踪情况。
方法：培养扩增大鼠骨髓间充质干细胞，第2代细胞按PKH26标记程序进行细胞标记，观察标记组和未标记组细胞的增殖、周期和凋亡情况，对标记组细胞行成骨成脂体外诱导分化。尾静脉移植PKH26标记细胞，6周后荧光显微镜观察骨髓间充质干细胞在子宫内膜的分布情况。
结果与结论：PKH26标记对细胞增殖、凋亡和周期无明显影响，不影响成骨成脂诱导分化。子宫内膜组织中PKH26标记阳性的细胞分布于腺上皮和间质细胞。PKH26标记技术可用于示踪骨髓间充质迁移转归和干细胞移植方面的实验研究。
 

关键词: 细胞标记, PKH26, 骨髓间充质干细胞, 移植, 迁移转归

Abstract:

BACKGROUND: Labeling of bone marrow mesenchymal stem cells (BMSCs) are important links to the research of differentiation and migration in vivo.
OBJECTIVE: To label the BMSCs with PKH26 and to explore the effect of PKH26 labeling on the biological activity, differentiation in vitro and tracking in vivo.
METHODS: BMSCs were isolated and cultivated from the bone marrow of rats. The passage 2 BMSCs were cultured and labeled with PKH26. The proliferation, cycle and apoptotic of cells in labeled group and unlabeled group were evaluated. The BMSCs in labeled group were performed with osteoblasts and lipoblasts induction in vitro. The PKH26-labeled BMSCs were transplanted through vein and the distribution of BMSCs in rat endometrium was observed under fluorescence microscope at 6 weeks after transplantation.
RESULTS AND CONCLUSION: The influence of PKH26-labelled cells on cell proliferation, apoptotic and cycle was not significant. And the labeled cells still had biological characteristics of osteoblasts and lipoblasts. In endometrium, PKH26-labelled cells were mainly distributed in epithelial cells and stromal cells. It suggested that the PKH26 labeling method could be used to study the homing, plasticity and transplantation of BMSCs.

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