中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2481-2486.doi: 10.3969/j.issn.1673-8225.2012.14.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞对肝癌模型大鼠血管形成的影响*★

宋  浩1，纪卫政2，邰沁文1，单骄宇3，张金辉1   

  1. 新疆医科大学第一附属医院，1肝脏腹腔镜外科，   2介入放射科，新疆维吾尔自治区乌鲁木齐市 830054；3 新疆医科大学基础医学院人体寄生虫学教研室，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2011-08-30 修回日期:2011-09-23 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 张金辉，主任医师，教授，硕士生导师，新疆医科大学第一附属医院肝脏腹腔镜外科，新疆维吾尔自治区乌鲁木齐市 830054 zhangjinhui2001@yahoo.com
  • 作者简介:宋浩★，男，1985年生，汉族，内蒙古自治区大兴安岭市人，新疆医科大学在读硕士，主要从事肝脏外科疾病方面的研究。 songhao.amos 2009@163.com
  • 基金资助:

    新疆医科大学第一附属医院科研基金(2009GXB02)。

Effects of bone marrow mesenchymal stem cells on vascularization of hepatoma models of rats

Song Hao1, Ji Wei-zheng2, Tai Qin-wen1, Shan Jiao-yu3, Zhang Jin-hui1     

  1. 1Department of Endoscope of Liver Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 2Department of Interventional Radiology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 3Department of Parasitology, Basic Medical School, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-08-30 Revised:2011-09-23 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Zhang Jin-hui, Chief physician, Professor, Master’s supervisor, Department of Endoscope of Liver Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zhangjinhui2001@yahoo.com
  • About author:Song Hao★, Studying for master’s degree, Department of Endoscope of Liver Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China songhao.amos2009@163.com
  • Supported by:

    Science Foundation of the First Affiliated Hospital of Xinjiang Medical University, No. 2009GXB02*

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摘要：

背景：骨髓间充质干细胞移植影响大鼠肝脏癌变过程中肝脏血管报道目前不多。
目的：观察骨髓间充质干细胞移植对大鼠肝脏癌变过程中血管生成的影响。
方法：将30只Wistar大鼠随机摸球法均分成骨髓间充质干细胞移植组、单纯造模组和空白对照组，前2组以二乙基亚硝胺为诱癌剂制备肝癌模型，空白对照组仅给予正常食水作对照。
结果与结论：单纯造模组8只大鼠形成肝癌，骨髓间充质干细胞移植组9只大鼠形成肝癌，肝脏携带SrY的阳性细胞，MRI影像中可见较多占位性病变，而单纯造模组占位病变数量明显较少，两组比较差异有显著性意义(t=2.45，P =0.026)；骨髓间充质干细胞移植组大鼠血管内皮细胞生长因子和微血管密度平均阳性表达率均高于单纯造模组(P < 0.05)。说明在二乙基亚硝胺制备的大鼠肝癌模型中，骨髓间充质干细胞可通过促进血管的生成而促进肝癌的生长。

关键词: 血管形成, 骨髓间充质干细胞, 大鼠, 肝癌, 血管, 移植

Abstract:

BACKGROUND: There are few reports about the effects of bone marrow mesenchymal stem cells (BMSCs) transplantation on vascularization during hepatocarcinogenesis in rats.
OBJECTIVE: To observe the effects of BMSCs transplantation on vascularization during hepatocarcinogenesis in rats.
METHODS: Thirty Wistar rats were divided into BMSCs transplantation group, pure model group and blank control group randomly. The first two groups were treated with diethylnitrosamine to prepare hepatoma models. The blank control group was treated with water as control.
RESULTS AND CONCLUSION: Eight rats in the pure model group and nine rats in the BMSCs transplantation group were proved to be with liver cancer, and the SrY+ cells were seen in the BMSCs transplantation group. MRI showed that there were more space-occupying lesions in the liver of the BMSCs transplantation group compared with the pure model group (t = 2.45, P = 0.026). The average positive expression rate of vascular endothelial growth factor and microvessel density in the BMSCs transplantation group were higher than those in the pure model group (P < 0.05). In the hepatoma models of rats prepared with diethylnitrosamine, BMSCs can promote the vascularization resulting in the development of liver cancer.

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