中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2163-2166.doi: 10.3969/j.issn.1673-8225.2012.12.017

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

不同哺乳动物红细胞溶血替代眼刺激实验的比较*★

敖华英1, 2，程树军2，谈伟君1   

  1. 1广东药学院公共卫生学院，广东省广州市  510310; 2广东出入境检验检疫技术中心，广东省广州市  510623
  • 收稿日期:2011-09-22 修回日期:2011-11-09 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 谈伟君，博士，教授，广东药学院公共卫生学院，广东省广州市 510310 tanwj0726@ yahoo.com.cn
  • 作者简介:敖华英★，女，1986年生，广东省阳江人，汉族，广东药学院在读硕士，主要从事眼刺激替代实验组合研究。 squeen900@ 163.com
  • 基金资助:

    广东省科技计划项目(2009B060300013)，课题名称：动物实验替代技术评估与共享。

Comparison of hemolysis tests instead of eye irritation testing in different mammalians

Ao Hua-ying1, 2, Cheng Shu-jun2, Tan Wei-jun1   

  1. 1School of Public Health, Guangdong Pharmaceutical University, Guangzhou  510310, Guangdong Province, China; 2Guangdong Entry-Exit Inspection and Quarantine Technology Center, Guangzhou  510623, Guangdong Province, China
  • Received:2011-09-22 Revised:2011-11-09 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Tan Wei-jun, Doctor, Professor, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong Province, China tanwj0726@ yahoo.com.cn
  • About author:Ao Hua-ying★, Studying for master’s degree, School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong Province, China; Guangdong Entry-Exit Inspection and Quarantine Technology Center, Guangzhou 510623, Guangdong Province, China squeen900@ 163.com
  • Supported by:

    Science and Technology Project of Guangdong Province, No. 2009B060300013*

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摘要：

背景：哺乳动物红细胞溶血实验单独或组合使用可替代体内Draize兔眼实验，用于化合物眼刺激性的标识、筛查或机制研究。但目前尚不清楚不同物种间的红细胞差异是否会对实验结果造成影响。
目的：比较大鼠和五指山小型猪血红细胞用于溶血实验的物质分类结果。
方法：分别制备两种动物的红细胞悬液，进行红细胞溶血实验。分析溶血和蛋白变性两个毒性终点，得到50%溶血浓度HD50、蛋白变性指数DI及H/D比值3个参数值。
结果与结论：经22种已知分类的眼刺激物检测表明，两种动物血样对化合物的分类差别无显著性意义(P > 0.05)。Wilcoxon符号秩和检验小型猪血与大鼠血对物质分类差异无显著性意义(S=8，P=0.304 7)，经Kappa一致性检验，得出小型猪血和大鼠血的一致性一般[Kappa=0.421 1，置信区间为(0.143 3，0.698 8)]。即种属差异对物质分类结果影响不大。
关键词：眼刺激；替代方法；红细胞溶血实验；大鼠；小型猪
doi:10.3969/j.issn.1673-8225.2012.12.017

关键词: 眼刺激, 替代方法, 红细胞溶血实验, 大鼠, 小型猪

Abstract:

BACKGROUND: Alone or in combination of mammalian hemolysis test can replace Draize rabbit test in vivo to be used in the identity, screening and mechanical research of compound eye irritation. However, whether the differences in red blood cells (RBCs) between different species can affect the results is unclear.
OBJECTIVE: To compare the substance classification of Wuzhishan micro-pig and rat red RBCs used in hemolysis test.
METHODS: The RBC suspension from rat blood and Wuzhishan micro-pig blood was prepared for RBC hemolysis test. Toxicity endpoints of hemolysis and protein denaturation were analyzed in order to gain the 50% hemolytic dosage (HD50), protein denaturation index (DI), and the ratio of HD50/DI.
RESULTS AND CONCLUSION: The eye irritation test of 22 known conference chemicals showed that the difference in substance classification of the two species of animals derived blood samples was not significant (P > 0.05). The difference was also not significant in the Wilcoxon signed rank test (S=8, P=0.304 7). The Kappa consistency test showed a mediocre accordance between the micro-pig blood and the rat blood [Kappa = 0.421 1, confidence interval (0.143 3, 0.698 8)]. It is indicated that the species differences contribute little to the results.

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