中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (11): 1989-1993.doi: 10.3969/j.issn.1673-8225.2012.11.021

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

贯穿缝扎联合打孔法构建兔耳难愈性创面模型*★

李  礼1，童亚林2，詹  球2，林  源1   

  1. 1广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021；2解放军第181医院烧伤整形外科，广西壮族自治区桂林市   541002
  • 收稿日期:2011-09-25 修回日期:2011-10-25 出版日期:2012-03-11 发布日期:2012-03-11
  • 通讯作者: 林源，主任医师，广西医科大学第一附属医院烧伤整形外科，广西壮族自治区南宁市 530021 gx-linyuan8@163.com
  • 作者简介:李礼★，男，1984年生，湖南省汨罗市人，汉族，广西医科大学在读硕士，医师，主要从事烧伤整形与创面修复研究。 chenfeng5891429@163.com
  • 基金资助:

    广西自然科学基金项目（桂科自0728255）。

Construction of a rabbit ear model of chronic wound using transfixion ligation combined punch method

Li Li1, Tong Ya-lin2, Zhan Qiu2, Lin Yuan1   

  1. 1Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2Department of Burns and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin  541002, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-25 Revised:2011-10-25 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Lin Yuan, Chief physician, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gx-linyuan8@163.com
  • About author:Li Li★, Studying for master’s degree, Physician, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China chenfeng5891429@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi, No. 0728255*

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摘要：

背景：以往制作的兔耳难愈创面模型仅局限于单纯的缺血模型，缺血淤血模型少见。
目的：建立一种方法简单、效果确实的兔耳难愈性创面模型。
方法：将40个兔耳分为两组：实验组贯穿缝扎兔耳根部背腹侧中央、头侧所有动、静脉，缝扎后第10天在耳背侧用活检打孔器制作3个深达软骨面的圆形创面。对照组仅制作与实验组完全相同的3个圆形创面。
结果与结论：实验组兔耳缝扎后立即呈现紫黑色，第10，20，30天为淡紫黑色、紫色、淡紫色，说明贯穿缝扎联合打孔法构建的难愈创面的缺血、淤血状态持久、稳定。实验组创面肉芽组织生长、表皮化时间及创面愈合时间均较长于对照组均延迟(P < 0.01)，血管内皮细胞生长因子表达明显低于对照组(P < 0.01)。说明贯穿缝扎联合打孔法制作的兔耳难愈性创面模型方法简单、效果确实可靠，可作为缺血淤血因素所致难愈性创面修复的实验研究模型。

关键词: 难愈性创面模型, 兔耳, 血管内皮细胞生长因子, 缺血, 淤血

Abstract:

BACKGROUND: In the past, the constructed rabbit ear models of chronic wound are limited to simple models of ischemia, while the model of both ischemia and congestion has been rarely reported.
OBJECTIVE: To establish a chronic wound model for ischemia and congestion using rabbit ears.
METHODS: A total of 40 rabbit ears were divided into two groups, experiment group and control group. The area of the dorsoventral side in ear root, all cephalic and center arteries and veins were transfixion ligated in the experiment group. Three circular wounds as deep as the cartilage surface were punched at the dorsal sides of the ears, using biopsy punch on the 10th day after ligation. Three circular wounds identical to that of the experiment group were punched in the control group.
RESULTS AND CONCLUSION: The color of rabbit ears in the experiment group was deep violet-black immediately after ligation, and was somewhat violet-black, violet and lilac on the 10th, 20th and 30th days after ligation. It illustrates that the status of ischemia and congestion is durable and stable in the chronic wound model constructed using transfixion ligation combined punch method. Compared with the control group, the time of granulation tissue growth, the time of wound healing and epidermidalization in the experiment group were obviously delayed (P < 0.01). The expression of vascular endothelial growth factor in the experiment group was obviously lower than that of the control group (P < 0.01).These findings demonstrate that rabbit ear models of chronic wound constructed using transfixion ligation combined punch method are simple and reliable. It can be used as an ideal animal study model in the research of chronic wounds repair caused by ischemia and congestion factors.

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