中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (6): 995-999.doi: 10.3969/j.issn.1673-8225.2011.06.011

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

脐血浆神经营养活性蛋白成分对CD34+细胞增殖分化的影响

张  丽1，2，李倩如2，杜  英2，杨  波3，李国喜4，胡  祥4   

  1. 1河南省红十字血液中心，河南省郑州市  450053
    2郑州大学基础医学院微生物学与免疫学教研室，河南省郑州市  450001
    3郑州大学第一临床医院神经外科，河南省郑州市  450052
    4深圳市北科细胞工程研究所，广东省深圳市  518000
  • 收稿日期:2010-09-11 修回日期:2010-11-09 出版日期:2011-02-05 发布日期:2011-02-05
  • 通讯作者: 杜英，博士，教授，硕士生导师，郑州大学基础医学院微生物学与免疫学教研室，河南省郑州市 450001 duying@zzu.edu.cn
  • 作者简介:张丽★，女，1979年生，河南省漯河市人，汉族，郑州大学基础医学院微生物学与免疫学专业在读硕士，主治医师，主要从事血液活性成分及应用研究。 lilyzhl79@163.com 并列第一作者：李倩如，女，1972年生，博士，副教授，主要从事干细胞分化与免疫调节研究。

Effects of neurotrophic factors in umbilical cord blood plasma on CD34+ cell proliferation and differentiation

Zhang Li1, 2, Li Qian-ru2, Du Ying2, Yang Bo3, Li Guo-xi4, Hu Xiang4   

  1. 1Henan Province Red Cross Blood Center, Zhengzhou  450053, Henan Province, China
    2Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou  450001, Henan Province, China
    3Department of Neurosurgery, First Clinical Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    4Shenzhen Beike Cell Engineering Institute, Shenzhen  518000, Guangdong Province, China
  • Received:2010-09-11 Revised:2010-11-09 Online:2011-02-05 Published:2011-02-05
  • Contact: Du Ying, Doctor, Professor, Master’s supervisor, Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China duying@zzu.edu.cn
  • About author:Zhang Li★, Studying for master’s degree, Attending physician, Henan Province Red Cross Blood Center, Zhengzhou 450053, Henan Province, China; Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China lilyzhl79@163.com Li Qian-ru, Doctor, Associate professor, Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China Zhang Li and Li Qian-ru contributed equally to this article.

PDF

441

被引次数

7

摘要：

背景：迄今为止，人们对于脐血浆中是否存在活性成分及其对于神经发育及神经损伤修复的作用认识尚不足，有待深入研究。
目的：检测脐血浆生物活性成分，观察其在神经发育及神经损伤修复中的作用。
方法：采用抗体芯片技术对脐血浆和健康青年女性静脉血浆活性成分进行对比分析，采用免疫磁珠从脐血中分选出CD34+细胞，经体外培养计数观察CD34+细胞增殖能力，通过免疫组化法观察细胞分化情况。
结果与结论：脐血浆中有31种蛋白分子的含量显著高于静脉血浆，其中具有促进神经再生和神经修复潜能的活性蛋白10种，包括FGF4、Frizzled-3、IL-3、RAGE、CRIM-1、Neuritin、Neuropilin-2、Neurturin、SFRP-3、Tomoregulin-1；在CD34+细胞培养液中加入脐血浆能显著促进细胞增殖，促进细胞向神经细胞分化。

关键词: 脐血浆, CD34+细胞, 神经营养因子, FGF4, Frizzled-3, Neutrin

Abstract:

BACKGROUND: Further studies are needed to understand if the neurotrophic factors in human umbilical cord blood plasma have the promoting effect on neural regeneration and repair.
OBJECTIVE: To investigate the component of neurotrophic factors in umbilical cord blood plasma, observe its application in neural regeneration and repair.
METHODS: The difference was compared between the human umbilical cord blood plasma (HUCBP) and peripheral venous blood plasma (PVBP) from healthy voluntary womenfolk using human antibody array. The CD34+ cells were sorted from human umbilical cord blood using immunomagnetic beads. Following cultivating in vitro and counting, proliferative ability of CD34+ cells was observed. Cell differentiation was observed using immunohistochemical method.
RESULTS AND CONCLUSION: The concentrations of 31 kinds of protein molecules were significantly higher in HUCBP than PVBP. Of them, 10 kinds of molecules thereinto have significant potential of nerve regeneration and plasticity, including FGF4, Frizzled-3, IL-3, RAGE, CRIM-1, Neuritin, Neuropilin-2, Neurturin, SFRP-3 and Tomoregulin-1. HUCBP may promote the CD34+ cells proliferation in cell culture medium and differentiation into nerve cells. 

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