中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7715-7718.doi: 10.3969/j.issn.1673-8225.2011.41.028

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

胚胎及出生后大鼠视网膜细胞促红细胞生成素的表达

孟旭霞,张  燕,牛膺筠   

  1. 青岛大学医学院附属医院眼科,山东省青岛市  266003
  • 收稿日期:2011-04-03 修回日期:2011-05-24 出版日期:2011-10-08 发布日期:2011-10-08
  • 作者简介:孟旭霞★,女,1962年生,山东省沂源县人,汉族,2003年毕业于青岛大学医学院,硕士,主任医师,主要从事玻璃体视网膜病的研究。
  • 基金资助:

    山东省青岛市科技局资金资助(09-1-1-18-nsh),低氧诱导因子与视网膜胚胎发育。

Retinal erythropoietin expression in embryonic and postnatal rats

Meng Xu-xia, Zhang Yan, Niu Ying-yun   

  1. Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China
  • Received:2011-04-03 Revised:2011-05-24 Online:2011-10-08 Published:2011-10-08
  • About author:Meng Xu-xia★, Master, Chief physician, Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China mengxuxia@163. com
  • Supported by:

    a grant from Qingdao Science and Technology Bureau, No. 09-1-1-18-nsh*

摘要:

背景:促红细胞生成素是低氧诱导因子1的下游靶基因,受低氧诱导因子1的调节。
目的:观察大鼠视网膜胚胎期及出生后早期发育过程中促红细胞生成素的时空表达与视网膜发育的关系。
方法:取胚胎12,16,20 d鼠胚及成年Wistar大鼠各5只,处死后摘除眼球,分离视网膜,用免疫组织化学方法、半定量反转录聚合酶联反应检测视网膜促红细胞生成素蛋白及促红细胞生成素 mRNA的表达。
结果与结论:胚胎期大鼠视网膜神经上皮及色素上皮细胞的胞浆及胞核内均有促红细胞生成素阳性表达,表达趋势随胎龄增长而逐渐减弱;成年鼠视网膜促红细胞生成素阳性表达最弱。说明大鼠视网膜胚胎发育过程中促红细胞生成素的表达存在时空上的变化,这种变化可能与大鼠视网膜胚胎期的发育密切相关。

关键词: 视网膜, 胚胎, 发育, 促红细胞生成素, 大鼠

Abstract:

BACKGROUND: Erythropoietin (EPO) is downstream target gene of hypoxia inducible factor-1 and regulated by hypoxia inducible factor-1.
OBJECTIVE: To investigate EPO expression during embryonic development of rat retina and postnatal early development and explore the role of EPO in retinal development. 
METHODS: Five embryo of 12, 16, 20 days and five adult Wistar rats were sacrificed. Their eyeballs were harvested and retina was separated. EPO protein and mRNA expression was detected by immunohistochemistry and RT-PCR, respectively. 
RESULTS AND CONCLUSION: EPO expression was detected in cytoplasm and nuclei of neuroepithelium of retina and pigment epithelial cells during embryonic development of rat retina. The expression was gradually decreased. EPO expression was the weakest in adult rat retina. Results showed that EPO expression exhibited spatial changes during embryonic development of rat retina, which may correlate closely to the embryonic development of rat retina.

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