中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7665-7668.doi: 10.3969/j.issn.1673-8225.2011.41.016

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

肝纤维化模型大鼠肝功能变化与萱草活性成分黄酮苷的干预

黄红焰,李玉白   

  1. 湖南环境生物学院,湖南省衡阳市   421005
  • 收稿日期:2011-04-23 修回日期:2011-06-21 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 李玉白,硕士,教授,湖南环境生物学院,湖南省衡阳市421005 Lyb8268838@ 163.com
  • 作者简介:黄红焰,男,1963年生,湖南省湘潭县人,汉族,1980年华中师范大学毕业,讲师,主要从事环境健康和高教管理研究。
  • 基金资助:

    衡阳市科技局基金项目(2007KS16) 。

Effect of orange daylily active component flavone glycosides on liver function in a rat model of hepatic fibrosis

Huang Hong-yan, Li Yu-bai   

  1. College of Hunan Environmental Biology, Hengyang  421005, Hunan Province, China
  • Received:2011-04-23 Revised:2011-06-21 Online:2011-10-08 Published:2011-10-08
  • Contact: Li Yu-bai, Professor, College of Hunan Environmental Biology, Hengyang 421005, Hunan Province, China Lyb8268838@163. com
  • About author:Huang Hong-yan, Lecturer, College of Hunan Environmental Biology, Hengyang 421005, Hunan Province, China
  • Supported by:

    a grant from Bureau of Science and Technology of Gengyang City, No. 2007KS16*

摘要:

背景:萱草活性成分黄酮苷被认为具有调整人体代谢,清除超氧阴离子自由基,清除体内代谢废物,护肝抗衰老,有很强的扶正固本之功效。
目的:观察萱草活性成分黄酮苷对肝纤维化模型大鼠的肝功能的影响。
方法:建造四氯化碳大鼠肝纤维化模型,建模的同时给予萱草活性成分黄酮苷干预。实验4周后采血,检测血清清蛋白、丙氨酸氨基转移酶、清球蛋白比值、高密度脂蛋白值,观察大鼠的进食、活动和生长状况。
结果与结论:实验4周后,四氯化碳模型组大鼠死亡率为37.5%,黄酮苷组为12.5%。2周后,模型组大鼠体质量明显下降(P < 0.05),黄酮苷组与阴性对照组比较未见显著性下降(P > 0.05)。黄酮苷组大鼠肝/体比值较模型组有所下降。黄酮苷较模型组血清清蛋白、丙氨酸氨基转移酶及清球蛋白比值显著降低,与阴性对照组差异无显著性意义。提示,在肝纤维化的病理进程中,采取萱草活性成分黄酮苷进行干预,可有效缓解大鼠肝损害改善肝功能。

关键词: 黄酮苷, 肝纤维化, 生化指标, 肝纤维化模型, 大鼠

Abstract:

BACKGROUND: Orange daylily active component flavone glycosides has been considered to be able to regulate organism metabolism, get rid of superoxide anion radicals and metabolic waste in vivo, improve liver function and delay the progress of hepatic fibrosis.
OBJECTIVE: To observe the effects of flavone glycosides on liver function in a rat model of hepatic fibrosis.
METHODS: Hepatic fibrosis models were developed by carbon tetrachloride (CCl4) induction, and flavone glycosides was given orally to rats. After 4-week experimentation, blood was selected and alanine aminotransferase (ALT), albumin (ALB), albumin/globulin ratio (A/G), high density lipoprotein (HDL) in serum were detected. Rat food intake, actitives and growth were observed. 
RESULTS AND CONCLUSION: After 4 week experimentation, the mortality was 37.5% and 12.5% in the CCl4 model group and flavone glycosides group, respectively. Another 2 weeks later, rat body mass was significantly decreased in the model group   (P < 0.05). Compared with the negative control group, rat body mass in the flavone glycosides group was not significantly decreased (P > 0.05). Compared with CCl4 model group, liver mass/body mass ratio was slighly decreased in the flavone glycosides group. Compared with CCl4 model group, serum level of ALT, ALB, A/G was significantly decreased in the flavone glycosides group. There was no significant difference in these indices between flavone glycosides group and negative control group. These findings suggest that during the pathological process of hepatic fibrosis, intervention with orange daylily active component flavone glycosides can effectively relieve rat liver damage and improve liver function.

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