中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7661-7664.doi: 10.3969/j.issn.1673-8225.2011.41.015

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

脑缺血再灌注损伤模型大鼠参附注射液干预后热休克蛋白70的表达

江承平1，刘  福1，李  毅1，王柏强1，唐晓蓉1，吴碧华2，王晓明2   

  1. 1川北医学院附属医院药剂科，四川省南充市  637007
    2川北医学院神经病学研究所，四川省南充市  637000
  • 收稿日期:2011-07-07 修回日期:2011-08-29 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 吴碧华，教授，博士，硕士生导师，川北医学院神经病学研究所，四川省南充市 637000
  • 作者简介:江承平，男，1967年生，四川省广安市人，汉族，1998年中国药科大学毕业，副主任药师，主要从事药物基础与临床研究。 chengpingjiang@sina.cn
  • 基金资助:

    四川省科技厅资助项目 (2010JY0094），课题名称：参附注射液对实验性脑缺血再灌注损伤保护作用及其机制。

Heat shock protein 70 expression after Shenfu injection administration in a rat model of cerebral ischemia/reperfusion injury   

Jiang Cheng-ping1, Liu Fu1, Li Yi1, Wang Bai-qiang1, Tang Xiao-rong1, Wu Bi-hua2, Wang Xiao-ming2   

  1. 1Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong  637007, Sichuan Province, China
    2Neurology Research Institute, North Sichuan Medical College, Nanchong  637007, Sichuan Province, China
  • Received:2011-07-07 Revised:2011-08-29 Online:2011-10-08 Published:2011-10-08
  • Contact: Wu Bi-hua, Professor, Doctor, Master’s supervisor, Neurology Research Institute, North Sichuan Medical College, Nanchong 637007, Sichuan Province, China
  • About author:Jiang Cheng-ping, Associate chief physician, Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China chengpingjiang@ sina.cn
  • Supported by:

    a grant from Department of Science and Technology of Sichuan Province, No. 2010JY0094*

PDF

326

被引次数

6

摘要：

背景：参附注射液可通过改善微循环，增加组织血氧含量发挥对缺血再灌注损伤的保护作用。
目的：观察参附注射液对大鼠脑缺血再灌注损伤后热休克蛋白70表达的影响。
方法：将SD大鼠随机分为3组：假手术组、大脑中动脉闭塞缺血再灌注损伤模型组、参附注射液组。
结果与结论：应用参附注射液1 d后，改善了缺血再灌注大鼠脑部神经细胞的排列，减轻了胞体肿胀，核固缩等现象，应用3 d后改善更为明显，胞体结构已较清晰，核固缩、溶解程度显著减轻，胞体肿胀现象明显改善。参附注射液组治疗后1，3 d热休克蛋白70表达明显高于假手术组与模型组。提示参附注射液对脑缺血再灌注具有显著的保护作用，其作用可能是通过促进热休克蛋白70的表达来实现的。

关键词: 参附注射液, 缺血再灌注损伤, 热休克蛋白70, 脑, 大鼠

Abstract:

BACKGROUND: Shenfu injection plays a protective effect on ischemia/reperfusion injury in improving microcirculation and increasing blood oxygen content in tissue.
OBJECTIVE: To investigate the effects of Shenfu injection on heat shock protein 70 (Hsp70) expression in a rat model of cerebral ischemia/reperfusion injury.
METHODS: SD rats were randomly divided into three groups (n=32 for each group): sham-operated, middle cerebral artery occlusion (MCAO), and Shenfu injection.
RESULTS AND CONCLUSION: Shenfu injection improved the arrangement of brain nerve cells, reduced the swelling of cell bodies and pyknosis at 1 day after administration. At 3 days after Shenfu injection administration, a more obvious trend on the attenuation of the above phenomena was observed. At 1 and 3 days, Hsp70 expression was significantly higher in the Shenfu injection group than in the sham-operated and MCAO groups. These finding suggest that Shenfu injection exhibits a significant protective effect on cerebral ischemia/reperfusion injury, and this protective effect may be implemented by increasing Hsp70 expression.

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