中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6863-6865.doi: 10.3969/j.issn.1673-8225.2011.37.006

• 骨组织构建 bone tissue construction • 上一篇    下一篇

深低温冷冻骨的形态学及免疫组织化学检测

夏武宪，郭  华，李龙腾，韩  芬，孙永武，张向军，张  帆   

  1. 郑州铁路职业技术学院，河南省郑州市 450052
  • 收稿日期:2011-01-21 修回日期:2011-02-11 出版日期:2011-09-10 发布日期:2011-09-10
  • 作者简介:夏武宪，男，1951年生，河南省汝州市人，汉族，1987年南京铁道医学院毕业，副教授，主要从事应用解剖学解剖学教学研究。 xiawuxian@ yahoo.com.cn
  • 基金资助:

     课题受河南省教育厅自然科学研究计划项目资助(2009C310014)，骨移植骨材料的研究及骨库的建立。

Morphological and immunohistochemical detection of deep-frozen bone

Xia Wu-xian, Gou Hua, Li Long-teng, Han Fen, Sun Yong-wu, Zhang Xiang-jun, Zhang Fan   

  1. Zhengzhou Railway Vocational Technical College, Zhengzhou 450052, Henan Province, China
  • Received:2011-01-21 Revised:2011-02-11 Online:2011-09-10 Published:2011-09-10
  • About author:Xia Wu-xian, Associate professor, Zhengzhou Railway Vocational Technical College, Zhengzhou 450052, Henan Province, China xiawuxian@yahoo. com.cn
  • Supported by:

    the Natural Science Research Plan Program of Education Department of Henan Province, No. 2009C310014*

PDF

409

被引次数

22

摘要：

背景：深低温冷冻技术能够降低同种异体骨的免疫原性，对修复骨缺损具有重要意义。
目的：观察深低温冷冻对骨材料中骨形态发生蛋白2表达的影响。
方法：18只新西兰兔随机分为3组，深低温冷冻3个月组，深低温冷冻6个月组，新鲜骨对照组，取右侧胫骨中上1/3，分别于-80 ℃保存保存3个月，6个月及不保存，进行苏木精-伊红染色和骨形态发生蛋白2免疫组织化学染色。
结果与结论：深低温冷冻后骨材料形态学，与新鲜骨差异不大；深低温冷冻3个月及6个月骨组织中骨形态发生蛋白2均有表达，但较新鲜骨降低。

关键词: 深低温冷冻, 移植, 形态学, 骨形态发生蛋白2, 免疫组织化学

Abstract:

BACKGROUND: Bone morphogenetic protein 2 (BMP-2) in fracture healing and repair process plays an important role in deep-freezing technology.
OBJECTIVE: To investigate the effects of deep-freezing technology on BMP-2 expression in the bone materials.
METHODS: 18 rabbits were randomly and evenly divided into three groups: deep-frozen 3 months, deep frozen 6 months, fresh bone. The middle upper 1/3 right tibia from each group was taken and preserved at -80 ℃ for 3 or 6 months or was not preserved. Then all bone samples were performed hematoxylin-eosin staining and BMP-2 immunohistochemical staining.
RESULTS AND CONCLUSION: There was no significant difference in morphology between deep frozen bone and fresh bone. BMP-2 was expressed in bone deep-frozen for 3 and 6 months, but the expression was lower than that in the fresh bone.

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