中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2521-2524.doi: 10.3969/j.issn.1673-8225.2011.14.012

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

体外培养大鼠脂肪干细胞诱导分化为神经细胞

高  华，王玉玲，姚亚妮，杨新玲   

  1. 新疆医科大学第一附属医院神经医学中心，新疆维吾尔自治区乌鲁木齐市  830054
  • 收稿日期:2010-10-15 修回日期:2011-02-20 出版日期:2011-04-02 发布日期:2013-11-02
  • 通讯作者: 杨新玲，博士，主任医师，教授，新疆医科大学第一附属医院神经医学中心，新疆维吾尔自治区乌鲁木齐市 830054 poplar862@sohu.com
  • 作者简介:高华★，女，1979年生，新疆维吾尔自治区伊犁巩留县人，汉族，新疆医科大学在读硕士，主要从事帕金森病方面的研究。 gaohuasnow@163.com

Induced differentiation of rat adipose stem cells into nerve cells in vitro

Gao Hua, Wang Yu-ling, Yao Ya-ni, Yang Xin-ling   

  1. Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2010-10-15 Revised:2011-02-20 Online:2011-04-02 Published:2013-11-02
  • Contact: Yang Xin-ling, Doctor, Chief physician, Professor, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China poplar862@sohu.com
  • About author:Gao Hua★, Studying for master’s degree, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China gaohuasnow@163.com

PDF

320

被引次数

8

摘要：

背景：脂肪间充质干细胞是一组具有多向分化潜能的干细胞，在不同诱导条件下可以向软骨细胞、骨细胞、脂肪细胞、心肌细胞分化，在一定条件下也可分化为神经干细胞。
目的：研究大鼠脂肪间充质干细胞的体外培养及细胞表型，探讨其向神经细胞方向分化的能力。
方法：取SD大鼠的腹股沟、附睾垫和腹膜后脂肪，分离培养脂肪间充质干细胞，形态观察并进行传代。流式细胞仪检测第三四代细胞表型CD29、CD44、CD45。利用碱性成纤维细胞生长因子，表皮生长因子模拟神经细胞生长环境，使脂肪间充质干细胞向神经细胞方向分化，免疫荧光法检测诱导分化后神经细胞nestin、NF-200、GFAP 的表达情况。
结果与结论：从大鼠的脂肪组织中成功提取脂肪间充质干细胞，并能连续传代，稳定增殖，高表达CD29(99.00±0.12)%，CD44(95.62±0.68)%，低表达CD45(0.13±0.12)%。经无血清的神经因子诱导后，脂肪间充质干细胞可表达nestin，加入血清后可表达NF-200、GFAP。提示脂肪间充质干细胞具有强大的体外扩增能力，体外建立神经诱导环境后可定向分化为神经细胞的生物学特性，可为神经系统损伤、退行性疾病提供种子细胞。

关键词: 脂肪间充质干细胞, 体外培养, SD大鼠, 分化, 细胞因子, 神经元

Abstract:

BACKGROUND: Adipose tissue-derived stem cells (ADSCs) have multiple differentiation potentials, which can differentiate into chondrocytes, osteocytes, adipose cells, and cardiac muscle cells under different induced conditions, in addition, they can also differentiated into nerve cells. 
OBJECTIVE: To research the in vitro culture and phenotype of rat ADSCs, and to explore it differentiation potential into nerve cells. 
METHODS: Inguinal, epididymis and post peritoneal fat tissues were gotten from rats, ADSCs were isolated and cultivated in media, and their shape was recorded passaged. The third or the forth passage cells were identified by stem cells common marker CD29, CD44 and CD45. ADSCs were induced to nerve cells in a simulated nerve cells habitat with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and then expressions of the neuron specific markers nestin, NF-200 and GFAP were determined with immunofluorescence.
RESULTS AND CONCLUSION: ADSCs were successfully extracted from rat fat tissues, and they could also passage infinitely in a considerable large amount. ADSCs strongly expressed CD29 (99.00±0.12)%, CD44 (95.62±0.68)% and low expressed CD45 (0.13±0.12)%. After neurolin induction without serum, induced ADSCs can express nestin; when have been added serum, most of induced cells expressed NF-200 and GFAP. These indicate that ADSCs have robust abilities of duplication. And it has a bionomics orientable differentiated into nerve cells in neural induction circumstance in vitro. ADSCs may be supplied as a source of cells for nervous system injuries and degenerative disease.

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