中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (14): 2517-2520.doi: 10.3969/j.issn.1673-8225.2011.14.011

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

前列腺素E1对人类脂肪源性基质细胞体外增殖的影响

杨焕纳1，崔正军1，Seung-Kyu Han2，Hyup-Woo Lee2，邹士波1，韩兆锋1，周  健1   

  1. 1郑州大学第一附属医院，河南省郑州市  450002
    2韩国高丽大学附属九老医院，152-703
  • 收稿日期:2011-01-19 修回日期:2011-03-02 出版日期:2011-04-02 发布日期:2013-11-02
  • 通讯作者: 崔正军，主任医师，教授，郑州大学第一附属医院，河南省郑州市 450002
  • 作者简介:杨焕纳★，女，1982年生，河南省安阳市人，汉族，2011年郑州大学毕业，硕士，医师，主要从事脂肪干细胞在组织工程中的应用研究。 yanghn05@163.com

Effects of prostaglandin E1 on in vitro proliferation of human adipose derived stromal cells 

Yang Huan-na1, Cui Zheng-jun1, Seung-Kyu Han2, Hyup-Woo Lee2, Zou Shi-bo1, Han Zhao-feng1, Zhou Jian1   

  1. 1First Affiliated Hospital of Zhengzhou University, Zhengzhou  450002, Henan Province, China
    2Korea University Medical Center Guro Hospital, 152-703
  • Received:2011-01-19 Revised:2011-03-02 Online:2011-04-02 Published:2013-11-02
  • Contact: Cui Zheng-jun, Professor, Chief physician, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, Henan Province, China
  • About author:Yang Huan-na★, Master, Physician, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450002, Henan Province, China yanghn05@163.com

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458

被引次数

1

摘要：

背景：近年来脂肪源性基质细胞成为研究与应用的热点之一，但前列腺素E1对脂肪源性基质细胞增殖的作用影响尚未见报道。
目的：观察前列腺素E1对人类脂肪源性基质细胞体外增殖的影响。
方法：从人正常脂肪组织分离培养脂肪源性基质细胞，用5种不同浓度的前列腺素E1处理培养的细胞，其中3×10-6，6×10-6，9×10-6，12×10-6 mol/L作为实验组，0×10-6 mol/L作为对照组，观察经不同浓度前列腺素E1处理后细胞的增殖情况。
结果与结论：不同浓度实验组的细胞数量与对照组相比均显著升高，差异有显著性意义(P < 0.05)，各实验组内相比，细胞数量增殖无显著性差异(P  > 0.05)。提示前列腺素E1具有促进脂肪源性基质细胞的增殖作用，且前列腺素E1的影响效果无剂量依赖性。

关键词: 前列腺素E1, 脂肪源性基质细胞, 细胞增殖, 脂肪, 干细胞

Abstract:

BACKGROUND: Recently, research and application of human adipose derived stromal cells (ADSCs) has become a hotspot; however, there are few reports addressing prostaglandin E1 (PGE1) effects on ADSCs proliferation.
OBJECTIVE: To explore the effects of PGE1 on ADSCs proliferation.
METHODS: ADSCs were obtained from human normal adipose tissue after isolation and culture. The cultured ADSCs were treated with five different concentration of PGE1, which were 3×10-6, 6×10-6, 9×10-6, 12×10-6 mol/L, and 0×10-6 mol/L (control group). After 3 days, the cell morphology and proliferation were observed.
RESULTS AND CONCLUSION: There was no obervious different among the experimental groups. And the cell number of each experimental group was obervious elevated, compared with that of the control group (P < 0.05). These findings indicated that ① PGE1 promoted the proliferation of ADSC; ② The effect of PGE1 had no dose dependent.

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