中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (7): 1179-1182.doi: 10.3969/j.issn.1673-8225.2010.07.009

• 神经组织构建 nerve tissue construction • 上一篇    下一篇

无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经修复坐骨神经缺损

张彩顺，吕  刚，张基仁   

  1. 辽宁医学院附属第一医院手外科，辽宁省锦州市   121001
  • 出版日期:2010-02-12 发布日期:2010-02-12
  • 通讯作者: 吕 刚，教授，博士生导师，辽宁医学院附属第一医院骨科，辽宁省锦州市 121001
  • 作者简介:张彩顺☆，男，1973年生，辽宁省丹东市人，汉族，2007年中国医科大学毕业，博士，副教授，主要从事周围神经组织工程方面的研究。zcs731009@163.com
  • 基金资助:

    课题受辽宁省教育厅高校创新团队项目(2008T114)资助。

Construction of tissue-engineered artificial nerve with the compound of acellular nerve graft and bone marrow mesenchymal stem cells to treat sciatic nerve defect

Zhang Cai-shun, Lü Gang, Zhang Ji-ren   

  1. Department of Hand Surgery, First Hospital of Liaoning Medical University, Jinzhou   121001, Liaoning Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Lü Gang, Professor, Doctoral supervisor, Department of Hand Surgery, First Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • About author:Zhang Cai-shun☆, Doctor, Associate professor, Department of Hand Surgery, First Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning Province, China zcs731009@163.com
  • Supported by:

    the High-School Innovation Team Project of the Education Department of Liaoning Province, No. 2008T114*

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623

被引次数

4

摘要：

背景：作者前期已经成功将无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经，并证明可以促进周围神经再生。
目的：构建组织工程人工神经，观察和验证桥接大鼠坐骨神经缺损后的神经功能恢复情况。
方法：成年雄性SD大鼠60只构建大鼠坐骨神经15 mm缺损模型。随机分成3组，每组20只。桥接大鼠坐骨神经缺损，实验组采用组织工程人工神经，空白对照组采用无细胞组织工程神经支架，自体神经对照组采用自体神经移植。桥接后12周通过大体观察、胫骨前肌湿质量、组织学等方法分析坐骨神经组织学及功能恢复情况。
结果与结论：桥接术后12周：实验组大鼠肢体可以支撑着地，钳夹大鼠手术侧足底皮肤出现逃避反射，足底皮肤s-100蛋白染色呈阳性反应。实验组与自体神经移植组胫骨前肌湿质量比差异无显著性意义(P > 0.05)。实验组辣根过氧化物酶逆行示踪实验显示脊髓、后根神经节均可见数量不等的辣根过氧化物酶标记阳性细胞。实验组移植物与自体神经移植组有髓神经纤维数、髓鞘厚度、神经组织面积比较差异无显著性意义。实验结果验证了无细胞神经移植物复合骨髓间充质干细胞构建组织工程人工神经修复大鼠坐骨神经缺损，可以促进神经组织学的修复重建和功能的恢复。

关键词: 人工神经, 坐骨神经缺损, 功能恢复, 神经组织工程, 大鼠

Abstract:

BACKGROUND: Tissue-engineered artificial nerve was successfully constructed with the compound of acellular nerve graft and bone marrow mesenchymal stem cells, suggesting that it could promote peripheral neural regeneration.
OBJECTIVE: To construct tissue-engineered artificial nerve, and to verify neural functional recovery of bridging rats following sciatic nerve defect.
METHODS: A total of 60 adult male SD rats were used to induce sciatic nerve defect models (15 mm in length), and they were then randomly divided into three groups, with 20 rats in each group. Sciatic nerve defect group was treated with tissue-engineered artificial nerve; blank control group was treated with tissue-engineered nerve stent; autoallergic neural control group was treated with autoallergic neural transplantation. Twelve weeks after bridging, histology of sciatic nerve and neural functional recovery were detected via gross observation, wet mass of tibialis anterior muscle, and histological analysis.
RESULTS AND CONCLUSION: At 12 weeks after bridging surgery, rats in experimental group were able to stand on the floor, and withdrawal reflex was detected at plantar skin on the surgical side. S-100 protein of plantar skin was positive. There was no significant difference in wet mass of tibialis anterior muscle between experimental and autoallergic neural transplantation group (P > 0.05). HRP retrograde tracing in the experimental group demonstrated that HRP-positive cells were observed in both spinal cord and posterior root ganglion. There was no significant difference in number of myelinated nerve fiber, thickness of myelin sheath, and area of nerve tissue between experimental and autoallergic neural transplantation group. The results demonstrated that the compound of acellular nerve graft and bone marrow mesenchymal stem cells could successfully construct tissue-engineered artificial nerve to repair sciatic nerve defect and promote neurohistological reconstruction and functional recovery.

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